Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   FLP15_RS01240 Genome accession   NZ_CP041356
Coordinates   252977..253999 (-) Length   340 a.a.
NCBI ID   WP_142767398.1    Uniprot ID   -
Organism   Lactococcus protaetiae strain KACC 19320     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 247977..258999
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLP15_RS01205 (FLP15_01205) - 249047..249889 (-) 843 WP_142765687.1 zinc ABC transporter substrate-binding protein -
  FLP15_RS01210 (FLP15_01210) - 250259..250717 (-) 459 WP_142765688.1 zinc-dependent MarR family transcriptional regulator -
  FLP15_RS01215 (FLP15_01215) comGG 251284..251580 (-) 297 WP_142765689.1 competence type IV pilus minor pilin ComGG Machinery gene
  FLP15_RS01220 (FLP15_01220) comGF 251609..252001 (-) 393 WP_142767397.1 competence type IV pilus minor pilin ComGF Machinery gene
  FLP15_RS01225 (FLP15_01225) comGE 252012..252308 (-) 297 WP_142765690.1 competence type IV pilus minor pilin ComGE Machinery gene
  FLP15_RS01230 (FLP15_01230) comGD 252280..252711 (-) 432 WP_142765691.1 competence type IV pilus minor pilin ComGD Machinery gene
  FLP15_RS01235 (FLP15_01235) comGC 252671..252973 (-) 303 WP_120772581.1 competence type IV pilus major pilin ComGC Machinery gene
  FLP15_RS01240 (FLP15_01240) comGB 252977..253999 (-) 1023 WP_142767398.1 competence type IV pilus assembly protein ComGB Machinery gene
  FLP15_RS01245 (FLP15_01245) comGA 253905..254888 (-) 984 WP_142765692.1 competence type IV pilus ATPase ComGA Machinery gene
  FLP15_RS13680 - 255681..255905 (-) 225 Protein_245 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 38612.33 Da        Isoelectric Point: 9.4277

>NTDB_id=371482 FLP15_RS01240 WP_142767398.1 252977..253999(-) (comGB) [Lactococcus protaetiae strain KACC 19320]
MDILQLLRLKRKKLSLIKQAKLIQLMANLTANGFHFGEIVEFLGLSHLVEQEFTLKLRAGLSAGQSLSEILEALNFSKNV
VTQVELVEFHGHLSETLSFIEQNLRQQLTIKKKLAGLLTYPIILLVFLVGIMLGLKNYLLPQLDSGRSWAMILINHLPVL
FVSSFVFLSVLTALGVVIFKKKPASRTFIFLARCPFLSDFVRLYLTAYFAREWGNLLAQGVDLKKILIIMRRQKSRIFSE
VGEVLTERLEAGVNFDVAVSELGFLAPELAIMIEYGAMKDKLGLELLLYSEECWEIFFAKVERAMQWIQPIVFIFVALMI
ILLYVAMLLPIYSNMGTMMG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=371482 FLP15_RS01240 WP_142767398.1 252977..253999(-) (comGB) [Lactococcus protaetiae strain KACC 19320]
ATGGATATCTTACAGCTATTGAGGTTGAAGCGGAAAAAGTTGAGTTTGATAAAACAAGCAAAATTGATTCAGTTGATGGC
AAATCTGACTGCTAATGGTTTTCATTTTGGCGAAATTGTTGAATTTCTTGGATTATCACATCTAGTTGAGCAAGAATTTA
CGTTGAAGTTGCGTGCGGGGCTTTCTGCTGGTCAATCTCTTTCCGAAATTTTGGAGGCATTAAATTTTTCTAAAAATGTA
GTGACTCAGGTTGAATTAGTTGAATTTCATGGACATTTGTCGGAGACTTTGTCATTTATTGAGCAAAATCTACGGCAACA
GTTGACGATAAAAAAGAAATTAGCTGGACTGTTAACTTATCCGATTATTTTACTTGTTTTTCTAGTAGGAATTATGTTGG
GTTTAAAAAATTATTTACTTCCACAGTTGGATTCTGGGAGAAGCTGGGCAATGATTTTGATTAATCATTTACCAGTACTT
TTTGTAAGTAGCTTTGTTTTTTTGTCAGTACTGACAGCTTTAGGAGTTGTTATTTTTAAGAAGAAACCAGCAAGTCGAAC
TTTTATTTTCTTAGCAAGATGTCCATTTCTGTCAGACTTTGTCCGTTTATATTTAACGGCTTATTTTGCAAGAGAATGGG
GAAATCTTTTGGCTCAAGGTGTGGATTTAAAGAAAATTTTAATCATCATGAGAAGACAAAAAAGTCGAATTTTTTCAGAG
GTGGGAGAAGTATTAACTGAGAGATTAGAAGCTGGGGTGAACTTTGATGTTGCGGTAAGTGAATTGGGGTTTTTAGCACC
GGAACTTGCGATAATGATTGAATATGGGGCGATGAAAGATAAGTTGGGCTTGGAACTTTTGCTATACTCGGAAGAGTGCT
GGGAAATTTTTTTTGCAAAAGTTGAACGAGCGATGCAGTGGATTCAGCCGATTGTTTTTATTTTTGTAGCGCTGATGATT
ATACTTTTATATGTGGCAATGCTATTACCAATTTATTCAAATATGGGAACAATGATGGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

62.5

98.824

0.618
  comGB/cglB Streptococcus mitis NCTC 12261

48.657

98.529

0.479
  comYB Streptococcus gordonii str. Challis substr. CH1

48.802

98.235

0.479
  comGB/cglB Streptococcus mitis SK321

48.358

98.529

0.476
  comGB/cglB Streptococcus pneumoniae TIGR4

47.463

98.529

0.468
  comGB/cglB Streptococcus pneumoniae D39

47.463

98.529

0.468
  comGB/cglB Streptococcus pneumoniae R6

47.463

98.529

0.468
  comGB/cglB Streptococcus pneumoniae Rx1

47.463

98.529

0.468
  comYB Streptococcus mutans UA159

47.785

92.941

0.444
  comYB Streptococcus mutans UA140

47.785

92.941

0.444

Multiple sequence alignment