Detailed information NTG0371478

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: FLP15_RS01220
• Genome accession: NZ_CP041356
• Coordinates: 251609..252001 (-)
• Length: 130 a.a.
• NCBI ID: WP_142767397.1
• Uniprot ID: A0A514ZBA1
• Organism: Lactococcus protaetiae strain KACC 19320
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 246609..257001

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FLP15_RS01195 (FLP15_01195)	-	246944..247753 (-)	810	WP_142765686.1	metal ABC transporter permease	-
FLP15_RS01200 (FLP15_01200)	-	247746..248476 (-)	731	Protein_235	metal ABC transporter ATP-binding protein	-
FLP15_RS01205 (FLP15_01205)	-	249047..249889 (-)	843	WP_142765687.1	zinc ABC transporter substrate-binding protein	-
FLP15_RS01210 (FLP15_01210)	-	250259..250717 (-)	459	WP_142765688.1	zinc-dependent MarR family transcriptional regulator	-
FLP15_RS01215 (FLP15_01215)	comGG	251284..251580 (-)	297	WP_142765689.1	competence type IV pilus minor pilin ComGG	Machinery gene
FLP15_RS01220 (FLP15_01220)	comGF	251609..252001 (-)	393	WP_142767397.1	competence type IV pilus minor pilin ComGF	Machinery gene
FLP15_RS01225 (FLP15_01225)	comGE	252012..252308 (-)	297	WP_142765690.1	competence type IV pilus minor pilin ComGE	Machinery gene
FLP15_RS01230 (FLP15_01230)	comGD	252280..252711 (-)	432	WP_142765691.1	competence type IV pilus minor pilin ComGD	Machinery gene
FLP15_RS01235 (FLP15_01235)	comGC	252671..252973 (-)	303	WP_120772581.1	competence type IV pilus major pilin ComGC	Machinery gene
FLP15_RS01240 (FLP15_01240)	comGB	252977..253999 (-)	1023	WP_142767398.1	competence type IV pilus assembly protein ComGB	Machinery gene
FLP15_RS01245 (FLP15_01245)	comGA	253905..254888 (-)	984	WP_142765692.1	competence type IV pilus ATPase ComGA	Machinery gene
FLP15_RS13680	-	255681..255905 (-)	225	Protein_245	helix-turn-helix transcriptional regulator	-

Sequence

Protein

• Length: 130 a.a.
• Molecular weight: 14628.03 Da
• Isoelectric Point: 8.2428

>NTDB_id=371478 FLP15_RS01220 WP_142767397.1 251609..252001(-) (comGF) [Lactococcus protaetiae strain KACC 19320]
MLECLVALLAISGSVLVISGLTQLLQQQMAKGQTNQQKDWQIFCEQMRGELEGTKLDDVSQNFLYITKSKALRYGLVGND
FRKTDAQGKGYQPMLFGIKSCQILDNMGVVKIMITFDSGGKRTFVYKFEE

Nucleotide

• Length: 393 bp

>NTDB_id=371478 FLP15_RS01220 WP_142767397.1 251609..252001(-) (comGF) [Lactococcus protaetiae strain KACC 19320]
ATGCTAGAATGTCTAGTCGCTCTTCTTGCAATATCTGGTTCTGTGCTTGTTATCTCTGGACTTACTCAACTCCTTCAGCA
GCAAATGGCTAAAGGACAGACGAATCAACAAAAAGATTGGCAAATTTTTTGCGAACAAATGCGCGGAGAGCTAGAAGGAA
CAAAACTTGATGATGTTTCTCAAAATTTTTTGTATATTACGAAAAGTAAAGCGTTGAGATATGGTTTAGTAGGCAATGAT
TTTCGCAAAACAGATGCTCAGGGAAAAGGATACCAGCCGATGCTTTTTGGGATAAAGTCTTGTCAAATACTAGATAATAT
GGGAGTGGTAAAAATCATGATAACTTTTGATAGTGGAGGGAAGCGGACATTTGTTTACAAATTTGAGGAGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (26-121)

3D structure

• AlphaFold DB: A0A514ZBA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	62.308	100	0.623
comYF	Streptococcus mutans UA159	43.443	93.846	0.408
comYF	Streptococcus mutans UA140	42.623	93.846	0.4
comGF/cglF	Streptococcus mitis NCTC 12261	38.636	100	0.392
comGF/cglF	Streptococcus mitis SK321	38.636	100	0.392
comGF/cglF	Streptococcus pneumoniae Rx1	37.121	100	0.377
comGF/cglF	Streptococcus pneumoniae D39	37.121	100	0.377
comGF/cglF	Streptococcus pneumoniae R6	37.121	100	0.377
comGF/cglF	Streptococcus pneumoniae TIGR4	37.121	100	0.377