Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FF962_RS28890 Genome accession   NZ_CP040684
Coordinates   6022693..6023142 (+) Length   149 a.a.
NCBI ID   WP_069953233.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain C79     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6017693..6028142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FF962_RS28865 (FF962_28870) yacG 6017856..6018056 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  FF962_RS28870 (FF962_28875) coaE 6018053..6018664 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  FF962_RS28875 (FF962_28880) pilD 6018661..6019533 (-) 873 WP_021264129.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  FF962_RS28880 (FF962_28885) pilC 6019537..6020757 (-) 1221 WP_023101565.1 type 4a pilus biogenesis protein PilC Machinery gene
  FF962_RS28885 (FF962_28890) pilB 6020761..6022461 (-) 1701 WP_021264132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FF962_RS28890 (FF962_28895) pilA 6022693..6023142 (+) 450 WP_069953233.1 pilin Machinery gene
  FF962_RS28900 (FF962_28905) nadC 6023344..6024192 (-) 849 WP_021264134.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FF962_RS28905 (FF962_28910) - 6024478..6026733 (+) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  FF962_RS28910 (FF962_28915) ampD 6026884..6027450 (+) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15567.84 Da        Isoelectric Point: 7.7818

>NTDB_id=366485 FF962_RS28890 WP_069953233.1 6022693..6023142(+) (pilA) [Pseudomonas aeruginosa strain C79]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGITGTNIKIGTDPSTATETYV
GVAANANKLGLIDVKIADTGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=366485 FF962_RS28890 WP_069953233.1 6022693..6023142(+) (pilA) [Pseudomonas aeruginosa strain C79]
ATGAAAGCTCAAAAAGGCTTTACTTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAATCCGCTGAAGACCACTGTTG
AAGAGTCTCTGTCGCGTGGAATTACTGGTACCAATATTAAAATTGGTACTGATCCATCTACTGCCACCGAAACATATGTT
GGGGTTGCTGCGAATGCCAACAAGCTGGGCTTGATTGATGTAAAGATTGCTGATACTGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACTTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAATCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

68.667

100

0.691
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.02

100

0.477
  pilA Acinetobacter baumannii strain A118

47.297

99.329

0.47
  pilA Vibrio cholerae C6706

42.949

100

0.45
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.949

100

0.45
  pilA Vibrio cholerae strain A1552

42.949

100

0.45
  pilA Vibrio parahaemolyticus RIMD 2210633

44.898

98.658

0.443
  comP Acinetobacter baylyi ADP1

42.484

100

0.436
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.755

95.973

0.43
  pilA Acinetobacter nosocomialis M2

41.429

93.96

0.389
  pilA2 Legionella pneumophila str. Paris

39.189

99.329

0.389
  pilA2 Legionella pneumophila strain ERS1305867

38.514

99.329

0.383

Multiple sequence alignment