Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E5E97_RS18830 Genome accession   NZ_CP038513
Coordinates   4062791..4063930 (+) Length   379 a.a.
NCBI ID   WP_011706550.1    Uniprot ID   A0AAI9Q2R8
Organism   Aeromonas sp. 2692-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4057791..4068930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5E97_RS18800 (E5E97_19000) - 4058098..4059162 (+) 1065 WP_042067836.1 3-deoxy-7-phosphoheptulonate synthase -
  E5E97_RS18805 (E5E97_19005) aac(6') 4059169..4059627 (+) 459 WP_171280770.1 aminoglycoside 6'-N-acetyltransferase -
  E5E97_RS18810 (E5E97_19010) - 4059649..4060434 (-) 786 WP_171280771.1 ATP-binding cassette domain-containing protein -
  E5E97_RS18815 (E5E97_19015) btuC 4060421..4061428 (-) 1008 WP_171280772.1 vitamin B12 ABC transporter permease BtuC -
  E5E97_RS18820 (E5E97_19020) - 4061529..4062260 (-) 732 WP_043119150.1 ABC transporter substrate-binding protein -
  E5E97_RS18825 (E5E97_19025) - 4062374..4062730 (+) 357 WP_005302630.1 DMT family protein -
  E5E97_RS18830 (E5E97_19030) pilU 4062791..4063930 (+) 1140 WP_011706550.1 type IVa pilus ATPase TapW Machinery gene
  E5E97_RS18835 (E5E97_19035) glgC 4064213..4065484 (+) 1272 WP_011706551.1 glucose-1-phosphate adenylyltransferase -
  E5E97_RS18840 (E5E97_19040) - 4065620..4066099 (+) 480 WP_171280773.1 WbuC family cupin fold metalloprotein -
  E5E97_RS18845 (E5E97_19045) - 4066219..4066461 (-) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  E5E97_RS18850 (E5E97_19050) feoB 4066458..4068731 (-) 2274 WP_171280774.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42264.32 Da        Isoelectric Point: 6.4290

>NTDB_id=354939 E5E97_RS18830 WP_011706550.1 4062791..4063930(+) (pilU) [Aeromonas sp. 2692-1]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTSDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=354939 E5E97_RS18830 WP_011706550.1 4062791..4063930(+) (pilU) [Aeromonas sp. 2692-1]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTGCGTCCGCTCAGTGAAACGCCGCTCGAGCCCGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAATCCACCTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTGCATCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
TGCCCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGTGAAATTCGCGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAGACCGGCCACCTGGCGATTTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGCCCCCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTCAAAACCAGCGATGGTGGTCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATTCGCGACATGATCA
AACGCGGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTCGATCTGGTGGTAGAGGGGGTGATCGACGAAGAGGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGATG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361

Multiple sequence alignment