Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMO_RS03625 Genome accession   NC_013016
Coordinates   707411..708541 (+) Length   376 a.a.
NCBI ID   WP_002232075.1    Uniprot ID   C6S635
Organism   Neisseria meningitidis alpha14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 702411..713541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMO_RS14130 - 703076..703225 (+) 150 WP_019273745.1 hypothetical protein -
  NMO_RS03610 (NMO_0654) lepB 703444..704463 (-) 1020 WP_015815401.1 signal peptidase I -
  NMO_RS03615 (NMO_0655) lepA 704606..706399 (-) 1794 WP_002225433.1 translation elongation factor 4 -
  NMO_RS03620 (NMO_0656) - 706542..707243 (-) 702 WP_002251217.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  NMO_RS03625 (NMO_0657) pilU 707411..708541 (+) 1131 WP_002232075.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMO_RS03630 (NMO_0658) - 708575..709552 (+) 978 WP_002222732.1 DNA polymerase III subunit delta' -
  NMO_RS03635 (NMO_0659) - 709556..709906 (+) 351 WP_002214009.1 PilZ domain-containing protein -
  NMO_RS03640 (NMO_0660) - 709911..710690 (+) 780 WP_014581897.1 TatD family hydrolase -
  NMO_RS03645 (NMO_0661) - 710716..710931 (+) 216 WP_002219531.1 DUF333 domain-containing protein -
  NMO_RS03650 (NMO_0662) grxD 711032..711343 (+) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  NMO_RS03660 (NMO_0663) upp 711449..712075 (+) 627 WP_002214002.1 uracil phosphoribosyltransferase -
  NMO_RS03665 (NMO_0664) - 712098..712418 (+) 321 WP_002214001.1 DUF2322 family protein -
  NMO_RS03670 (NMO_0665) - 712669..713091 (+) 423 WP_002219529.1 protein Gly1ORF1 -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.32 Da        Isoelectric Point: 6.2885

>NTDB_id=34963 NMO_RS03625 WP_002232075.1 707411..708541(+) (pilU) [Neisseria meningitidis alpha14]
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=34963 NMO_RS03625 WP_002232075.1 707411..708541(+) (pilU) [Neisseria meningitidis alpha14]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACCAAAACAAAGGTTCCGA
CCTGTTCGTGACGACCCATTTCCCGCCCGCGATGAAGTTGGACGGCAAAATCACCCGCATCACGGACGAGCCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGTCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGATTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACGATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGATCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCCTGCAAGAAGCATTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6S635

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.143

91.223

0.521
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Acinetobacter baylyi ADP1

43.03

87.766

0.378
  pilT Legionella pneumophila strain Lp02

43.519

86.17

0.375
  pilT Legionella pneumophila strain ERS1305867

43.519

86.17

0.375
  pilT Pseudomonas aeruginosa PAK

42.378

87.234

0.37
  pilT Acinetobacter baumannii strain A118

42.593

86.17

0.367
  pilT Acinetobacter nosocomialis M2

42.593

86.17

0.367
  pilT Acinetobacter baumannii D1279779

42.593

86.17

0.367
  pilT Neisseria meningitidis 8013

40.597

89.096

0.362
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment