Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ET951_RS07910 Genome accession   NZ_CP035682
Coordinates   1680674..1681123 (-) Length   149 a.a.
NCBI ID   WP_000649327.1    Uniprot ID   -
Organism   Vibrio mimicus strain 2011V-1073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1675674..1686123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET951_RS07890 coaE 1676178..1676786 (-) 609 WP_192927859.1 dephospho-CoA kinase -
  ET951_RS07895 pilD 1676783..1677658 (-) 876 WP_154113250.1 prepilin peptidase Machinery gene
  ET951_RS07900 pilC 1677721..1678947 (-) 1227 WP_000648442.1 type II secretion system F family protein Machinery gene
  ET951_RS07905 pilB 1678986..1680674 (-) 1689 WP_154113251.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ET951_RS07910 pilA 1680674..1681123 (-) 450 WP_000649327.1 pilin Machinery gene
  ET951_RS07915 nadC 1681368..1682258 (-) 891 WP_005514794.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ET951_RS07920 ampD 1682424..1682972 (+) 549 WP_154113252.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ET951_RS07925 fldB 1683028..1683546 (-) 519 WP_154113253.1 flavodoxin FldB -
  ET951_RS07930 xerD 1683714..1684592 (+) 879 WP_031343804.1 site-specific tyrosine recombinase XerD -
  ET951_RS07935 dsbC 1684609..1685361 (+) 753 WP_154113254.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15409.52 Da        Isoelectric Point: 9.6276

>NTDB_id=343609 ET951_RS07910 WP_000649327.1 1680674..1681123(-) (pilA) [Vibrio mimicus strain 2011V-1073]
MKAYKNKQQKGFTLIELMIVVAVIGVLAAIAVPQYQKYVAKSEAASALATLTGLKTNAEAYTVETGSFPTNNENAQLGTP
TSSMGTIAYANGGNSGAAGTITFSFTGTVSPDLKNKGIRLSRTATGTWTCESATALDVGVRPKGCSTAY

Nucleotide


Download         Length: 450 bp        

>NTDB_id=343609 ET951_RS07910 WP_000649327.1 1680674..1681123(-) (pilA) [Vibrio mimicus strain 2011V-1073]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTAATTGGTGTATT
GGCAGCAATTGCAGTGCCACAATACCAGAAATACGTAGCTAAAAGTGAAGCTGCTTCTGCGTTAGCTACGTTAACTGGCT
TAAAAACGAATGCAGAAGCTTATACTGTTGAAACAGGATCTTTTCCAACAAATAACGAAAATGCTCAACTAGGAACACCT
ACCTCTTCCATGGGTACTATTGCTTATGCGAATGGAGGAAATAGTGGTGCAGCAGGAACAATAACATTCTCCTTTACTGG
TACTGTTAGCCCAGATCTAAAAAATAAAGGAATTAGACTATCTAGAACAGCTACTGGAACTTGGACTTGCGAATCAGCAA
CTGCATTAGATGTAGGAGTTCGCCCTAAAGGTTGTTCAACTGCATACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

50.327

100

0.517
  pilA Vibrio cholerae C6706

50.327

100

0.517
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50.327

100

0.517
  pilA Pseudomonas aeruginosa PAK

49.007

100

0.497
  pilA Vibrio parahaemolyticus RIMD 2210633

44.295

100

0.443
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.94

100

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.379

97.315

0.403
  pilA Acinetobacter nosocomialis M2

40.816

98.658

0.403
  pilA Acinetobacter baumannii strain A118

42.446

93.289

0.396
  pilA/pilA1 Eikenella corrodens VA1

34.545

100

0.383

Multiple sequence alignment