Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ET951_RS07905 Genome accession   NZ_CP035682
Coordinates   1678986..1680674 (-) Length   562 a.a.
NCBI ID   WP_154113251.1    Uniprot ID   -
Organism   Vibrio mimicus strain 2011V-1073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1673986..1685674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET951_RS07880 yacG 1674958..1675155 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  ET951_RS07885 zapD 1675406..1676146 (-) 741 WP_070381366.1 cell division protein ZapD -
  ET951_RS07890 coaE 1676178..1676786 (-) 609 WP_192927859.1 dephospho-CoA kinase -
  ET951_RS07895 pilD 1676783..1677658 (-) 876 WP_154113250.1 prepilin peptidase Machinery gene
  ET951_RS07900 pilC 1677721..1678947 (-) 1227 WP_000648442.1 type II secretion system F family protein Machinery gene
  ET951_RS07905 pilB 1678986..1680674 (-) 1689 WP_154113251.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ET951_RS07910 pilA 1680674..1681123 (-) 450 WP_000649327.1 pilin Machinery gene
  ET951_RS07915 nadC 1681368..1682258 (-) 891 WP_005514794.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ET951_RS07920 ampD 1682424..1682972 (+) 549 WP_154113252.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ET951_RS07925 fldB 1683028..1683546 (-) 519 WP_154113253.1 flavodoxin FldB -
  ET951_RS07930 xerD 1683714..1684592 (+) 879 WP_031343804.1 site-specific tyrosine recombinase XerD -
  ET951_RS07935 dsbC 1684609..1685361 (+) 753 WP_154113254.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62319.35 Da        Isoelectric Point: 5.8729

>NTDB_id=343608 ET951_RS07905 WP_154113251.1 1678986..1680674(-) (pilB) [Vibrio mimicus strain 2011V-1073]
MLTNLVAILRQAELISSTQEQAVIEQVSASGTSVPEALLELSIFHAQELTEQISHIFGLPETNLSHYDYPNLCQQLGLRE
LITRYEALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGATNQGKEISQDELAN
LVKVGDEERQSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYEAQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNCDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKVAVRPSAILQNQFAFQPQE
ILYEANTSGCNECTGGYSGRVGIYEVMAFNTELAEAIMRRATIHQIESLAKNNGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=343608 ET951_RS07905 WP_154113251.1 1678986..1680674(-) (pilB) [Vibrio mimicus strain 2011V-1073]
ATGCTCACTAACCTTGTTGCTATCTTGCGTCAAGCTGAGCTGATTAGCTCGACGCAAGAACAAGCTGTGATCGAACAAGT
TAGTGCTTCTGGTACTTCAGTGCCGGAAGCGCTGCTAGAATTAAGTATTTTCCACGCGCAAGAACTCACCGAACAAATCA
GCCATATTTTCGGCTTACCGGAAACCAATTTAAGCCACTACGATTACCCAAATCTCTGCCAACAACTTGGTTTACGCGAG
CTGATTACCCGTTATGAAGCGCTCCCCATTGCCAAACAAGGCAATTTGCTGCTGCTCGCGGTTTCTGACCCCACTTTATT
ACAAGCCGAAGAAGAATTTCGCTTTGCTACCGGATTACAAGTGGAACTTGCCCTTGCTGATCATCGCGCTCTGCAAGCTG
CGATTCGCCGTTTGTATGGCCGCTCCATTCAGGGTGCTACCAACCAAGGTAAAGAGATCAGCCAAGATGAATTAGCCAAT
CTAGTCAAAGTCGGGGATGAAGAACGTCAGTCAATTGAAGATTTAAGCCAAGATGATTCTCCGGTCAGCCGCTTTATCAA
CCAAATATTGCTGGATGCGGTACGTAAAGGCGCATCCGACATTCATTTCGAGCCTTATGAAGCGCAATACCGCATTCGCC
TGCGCTGCGATGGCATTTTGGTGGAAACCCAACAACCTGCCAGCCATTTAAGCCGCCGCTTAGCCGCCCGAATTAAGATT
CTCTCTAAACTGGACATTGCTGAACGCCGCTTGCCACAAGATGGCCGAATCAAGTTACGCCTTAATTGTGATACCGCGAT
TGATATGCGCGTATCAACTTTACCCACCTTGTGGGGTGAAAAAATTGTATTGCGCCTGCTCGATAGCAGCGCTGCGAATC
TTGATATTGATAAGCTCGGTTACAATCCTCAGCAGAAGCAACTTTATCTCAATGCACTCAAAAGGCCGCAAGGAATGATA
TTGATGACGGGGCCGACTGGTAGCGGAAAAACCGTCTCACTCTATACTGGGCTGCGCATTCTCAATACCTCACAAATTAA
TATTTCTACCGCCGAAGATCCGGTTGAAATTAACCTCTCTGGCATTAACCAAGTCCAGGTGCAACCGAAAATTGGTTTTG
GCTTTGCCGAAGCACTACGTTCTTTTCTACGTCAAGACCCCGACGTGGTCATGGTGGGCGAAATTCGCGATTTGGAAACC
GCAGAAATTGCGGTCAAAGCGGCGCAAACGGGTCACTTGGTGCTTTCAACTCTGCATACCAACTCAGCAGCAGAAACTGT
CATTCGCTTAGCCAACATGGGGGTTGAACCCTTTAACCTCGCTTCATCACTTAGTTTGATTATCGCCCAGCGCCTTGCTC
GCCGCTTATGTAAACACTGCAAAGTGGCGGTACGTCCGTCAGCCATACTGCAAAATCAGTTTGCCTTTCAACCTCAAGAA
ATTTTGTATGAAGCGAATACATCAGGCTGCAACGAATGCACCGGTGGTTATTCAGGGCGAGTCGGGATCTATGAAGTCAT
GGCATTTAATACTGAACTGGCAGAGGCCATTATGCGCCGTGCCACCATTCACCAAATTGAAAGTTTAGCCAAAAACAATG
GTATGCAAACCCTGCAAGAGTCCGGCCTTGAAAAGCTGCGTGAAGGCATCACCAGTTTTGCCGAGCTGCAACGTGTGCTT
TATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

95.374

100

0.954
  pilB Vibrio parahaemolyticus RIMD 2210633

73.665

100

0.737
  pilB Vibrio campbellii strain DS40M4

73.132

100

0.731
  pilB Legionella pneumophila strain ERS1305867

49.369

98.754

0.488
  pilB Acinetobacter baylyi ADP1

50.386

92.171

0.464
  pilB Acinetobacter baumannii D1279779

50.096

92.349

0.463
  pilF Neisseria gonorrhoeae MS11

45.922

100

0.461
  pilF Thermus thermophilus HB27

39.556

96.263

0.381
  pilB Haemophilus influenzae 86-028NP

39.394

93.95

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

39.845

91.993

0.367
  pilB Haemophilus influenzae Rd KW20

38.826

93.95

0.365

Multiple sequence alignment