Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ENC22_RS06685 Genome accession   NZ_CP034836
Coordinates   1492993..1493436 (+) Length   147 a.a.
NCBI ID   WP_127969023.1    Uniprot ID   A0AAE5XFX8
Organism   Hahella sp. KA22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1487993..1498436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ENC22_RS06665 (ENC22_06665) coaE 1488145..1488771 (-) 627 WP_127969019.1 dephospho-CoA kinase -
  ENC22_RS06670 (ENC22_06670) pilD 1488791..1489669 (-) 879 WP_127969020.1 A24 family peptidase Machinery gene
  ENC22_RS06675 (ENC22_06675) pilC 1489736..1490956 (-) 1221 WP_127969021.1 type II secretion system F family protein Machinery gene
  ENC22_RS06680 (ENC22_06680) pilB 1490960..1492672 (-) 1713 WP_127969022.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ENC22_RS06685 (ENC22_06685) pilE 1492993..1493436 (+) 444 WP_127969023.1 pilin Machinery gene
  ENC22_RS06690 (ENC22_06690) - 1493615..1495411 (+) 1797 WP_127969024.1 hypothetical protein -
  ENC22_RS06695 (ENC22_06695) - 1495444..1496508 (+) 1065 WP_127969025.1 UDP-N-acetylglucosamine 2-epimerase -
  ENC22_RS06700 (ENC22_06700) - 1496505..1497683 (+) 1179 WP_127969026.1 CDP-glycerol glycerophosphotransferase family protein -
  ENC22_RS06705 (ENC22_06705) - 1497661..1498065 (-) 405 WP_127969027.1 adenylyltransferase/cytidyltransferase family protein -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15193.59 Da        Isoelectric Point: 8.4476

>NTDB_id=333334 ENC22_RS06685 WP_127969023.1 1492993..1493436(+) (pilE) [Hahella sp. KA22]
MKSMQKGFTLIELMIVVAIIGILAAVAIPAYQDYTARAQISEGLSLSGGLKTTITEIWTQEGSLATADSGSSGIAAESDI
SGKYVEKVNVTDGLVTITMKSTGVAQAIQGKTYNISPVTGVGSIKWACKAGAAATKIDVKYLPKSCS

Nucleotide


Download         Length: 444 bp        

>NTDB_id=333334 ENC22_RS06685 WP_127969023.1 1492993..1493436(+) (pilE) [Hahella sp. KA22]
ATGAAGAGCATGCAAAAAGGTTTTACCCTTATTGAATTAATGATCGTTGTTGCGATCATTGGTATTTTGGCCGCTGTAGC
GATTCCTGCTTATCAGGATTACACTGCTCGCGCTCAGATATCAGAAGGGTTGTCGCTGTCTGGCGGTTTGAAAACTACTA
TTACTGAGATTTGGACTCAGGAAGGTTCTCTGGCTACTGCAGACAGTGGTAGCTCCGGTATTGCAGCTGAGTCAGACATC
AGCGGCAAATATGTCGAAAAAGTGAACGTTACTGATGGTTTGGTGACTATTACGATGAAGAGCACCGGCGTAGCTCAAGC
TATCCAAGGCAAAACTTATAACATCAGCCCTGTTACTGGCGTTGGCTCAATCAAGTGGGCTTGTAAAGCAGGCGCTGCCG
CTACCAAAATCGACGTTAAGTATCTGCCTAAGAGCTGCAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)

Pilin (bacterial filament) (Pilin)

(36-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

48.734

100

0.524
  pilE Neisseria gonorrhoeae MS11

45.625

100

0.497
  pilA/pilA1 Eikenella corrodens VA1

43.59

100

0.463
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.759

100

0.442
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.243

100

0.435
  pilA Ralstonia pseudosolanacearum GMI1000

38.182

100

0.429
  comP Acinetobacter baylyi ADP1

40

100

0.408
  pilA2 Legionella pneumophila str. Paris

41.379

98.639

0.408
  pilA Pseudomonas aeruginosa PAK

38.562

100

0.401
  pilA2 Legionella pneumophila strain ERS1305867

40.278

97.959

0.395
  pilA Acinetobacter baumannii strain A118

39.583

97.959

0.388
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.424

100

0.374

Multiple sequence alignment