Detailed information    

insolico Bioinformatically predicted

Overview

Name   comP   Type   Machinery gene
Locus tag   ELR50_RS12655 Genome accession   NZ_CP034688
Coordinates   2865784..2866230 (-) Length   148 a.a.
NCBI ID   WP_082656964.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain WXP-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2850394..2870259 2865784..2866230 within 0


Gene organization within MGE regions


Location: 2850394..2870259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELR50_RS12550 (ELR50_12475) - 2850394..2851590 (+) 1197 WP_269964071.1 type II toxin-antitoxin system HipA family toxin -
  ELR50_RS12555 (ELR50_12480) - 2851778..2851927 (-) 150 WP_237770692.1 DUF433 domain-containing protein -
  ELR50_RS12560 (ELR50_12485) - 2852075..2852416 (-) 342 WP_043318088.1 type II toxin-antitoxin system HicB family antitoxin -
  ELR50_RS12565 (ELR50_12490) - 2852413..2852664 (-) 252 WP_269964072.1 type II toxin-antitoxin system HicA family toxin -
  ELR50_RS12570 (ELR50_12500) - 2852911..2853984 (-) 1074 WP_269964073.1 diguanylate cyclase -
  ELR50_RS12575 (ELR50_12505) - 2854304..2855518 (+) 1215 WP_269964074.1 site-specific integrase -
  ELR50_RS12580 (ELR50_12510) - 2855515..2856210 (-) 696 WP_156060094.1 hypothetical protein -
  ELR50_RS12585 (ELR50_12515) - 2856209..2856409 (+) 201 WP_043272284.1 helix-turn-helix domain-containing protein -
  ELR50_RS12590 - 2856406..2856534 (+) 129 WP_256204891.1 hypothetical protein -
  ELR50_RS12595 (ELR50_12520) - 2856531..2856800 (+) 270 WP_269964075.1 hypothetical protein -
  ELR50_RS12600 (ELR50_12525) - 2856797..2857099 (+) 303 WP_269964076.1 hypothetical protein -
  ELR50_RS12605 (ELR50_12530) - 2857223..2857423 (+) 201 WP_043272281.1 helix-turn-helix domain-containing protein -
  ELR50_RS12610 (ELR50_12535) - 2857477..2857869 (+) 393 WP_218187152.1 hypothetical protein -
  ELR50_RS12615 (ELR50_12540) - 2858763..2859989 (+) 1227 WP_269964077.1 phage major capsid protein -
  ELR50_RS12620 (ELR50_12545) - 2860148..2860348 (+) 201 WP_043272279.1 hypothetical protein -
  ELR50_RS12625 (ELR50_12550) - 2860358..2860699 (+) 342 WP_269964078.1 HNH endonuclease -
  ELR50_RS12630 (ELR50_12555) - 2860770..2861252 (+) 483 WP_043272278.1 HK97-gp10 family putative phage morphogenesis protein -
  ELR50_RS12635 (ELR50_12560) - 2861262..2863616 (+) 2355 WP_269964079.1 phage tail tape measure protein -
  ELR50_RS12645 (ELR50_12570) - 2863997..2864650 (-) 654 WP_082656963.1 methyltransferase family protein -
  ELR50_RS12650 (ELR50_12575) - 2864659..2865630 (-) 972 WP_152989201.1 hypothetical protein -
  ELR50_RS12655 (ELR50_12580) comP 2865784..2866230 (-) 447 WP_082656964.1 pilin Machinery gene
  ELR50_RS12660 (ELR50_12585) pilB 2866460..2868163 (+) 1704 WP_269964080.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ELR50_RS12665 (ELR50_12590) pilC 2868167..2869384 (+) 1218 WP_269964081.1 type II secretion system F family protein Machinery gene
  ELR50_RS12670 (ELR50_12595) pilD 2869387..2870259 (+) 873 WP_269964082.1 prepilin peptidase Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 14896.06 Da        Isoelectric Point: 8.1003

>NTDB_id=332536 ELR50_RS12655 WP_082656964.1 2865784..2866230(-) (comP) [Pseudomonas citronellolis strain WXP-4]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRSRVAELAVMASGMKATVGENIANAGAIGAGACNGVSTIASANAT
KNTSSSSCTDTTGVIAVTGTDAAKNVVLTYTPTLNATTGVISWACTTASTNYKYVPAECRNTAAAPSP

Nucleotide


Download         Length: 447 bp        

>NTDB_id=332536 ELR50_RS12655 WP_082656964.1 2865784..2866230(-) (comP) [Pseudomonas citronellolis strain WXP-4]
ATGAAAGCTCAGAAAGGTTTCACTCTGATCGAGTTGATGATCGTTGTGGCGATCATCGGTATTTTGGCTGCAATTGCTCT
GCCTGCTTACCAAGACTATACCGTGCGTTCTCGAGTTGCTGAATTGGCAGTTATGGCGTCCGGTATGAAGGCTACCGTTG
GTGAAAATATTGCTAACGCGGGTGCTATTGGGGCTGGTGCTTGTAATGGTGTCAGCACTATCGCTAGCGCTAACGCGACG
AAGAACACCTCCTCTAGCTCTTGTACCGATACTACCGGGGTGATTGCAGTAACCGGTACTGATGCGGCTAAGAACGTGGT
CCTGACTTACACTCCCACTCTCAATGCTACTACTGGGGTGATTTCCTGGGCTTGTACTACGGCATCTACTAATTATAAGT
ATGTGCCGGCTGAATGCCGTAACACCGCAGCAGCACCCTCGCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Acinetobacter baylyi ADP1

55.705

100

0.561
  pilA Ralstonia pseudosolanacearum GMI1000

43.21

100

0.473
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.508

100

0.466
  pilA2 Legionella pneumophila str. Paris

48.905

92.568

0.453
  pilA2 Legionella pneumophila strain ERS1305867

48.905

92.568

0.453
  pilA/pilA1 Eikenella corrodens VA1

41.06

100

0.419
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.958

95.946

0.412
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.865

100

0.399
  pilE Neisseria gonorrhoeae strain FA1090

36.646

100

0.399
  pilA Pseudomonas aeruginosa PAK

36.538

100

0.385
  pilA Acinetobacter baumannii strain A118

37.086

100

0.378
  pilA Haemophilus influenzae 86-028NP

40.441

91.892

0.372

Multiple sequence alignment