Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ELR50_RS12660 Genome accession   NZ_CP034688
Coordinates   2866460..2868163 (+) Length   567 a.a.
NCBI ID   WP_269964080.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain WXP-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2861460..2873163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELR50_RS12645 (ELR50_12570) - 2863997..2864650 (-) 654 WP_082656963.1 methyltransferase family protein -
  ELR50_RS12650 (ELR50_12575) - 2864659..2865630 (-) 972 WP_152989201.1 hypothetical protein -
  ELR50_RS12655 (ELR50_12580) comP 2865784..2866230 (-) 447 WP_082656964.1 pilin Machinery gene
  ELR50_RS12660 (ELR50_12585) pilB 2866460..2868163 (+) 1704 WP_269964080.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ELR50_RS12665 (ELR50_12590) pilC 2868167..2869384 (+) 1218 WP_269964081.1 type II secretion system F family protein Machinery gene
  ELR50_RS12670 (ELR50_12595) pilD 2869387..2870259 (+) 873 WP_269964082.1 prepilin peptidase Machinery gene
  ELR50_RS12675 (ELR50_12600) coaE 2870333..2870941 (+) 609 WP_009617624.1 dephospho-CoA kinase -
  ELR50_RS12680 (ELR50_12605) yacG 2870938..2871147 (+) 210 WP_116422665.1 DNA gyrase inhibitor YacG -
  ELR50_RS12685 (ELR50_12610) - 2871422..2871616 (+) 195 WP_009617620.1 hypothetical protein -
  ELR50_RS12690 (ELR50_12615) - 2871616..2871891 (+) 276 WP_024128153.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
  ELR50_RS12695 (ELR50_12620) - 2871898..2872113 (-) 216 WP_043272266.1 hypothetical protein -
  ELR50_RS12700 (ELR50_12625) - 2872221..2872937 (-) 717 WP_269964083.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62591.58 Da        Isoelectric Point: 5.4556

>NTDB_id=332537 ELR50_RS12660 WP_269964080.1 2866460..2868163(+) (pilB) [Pseudomonas citronellolis strain WXP-4]
MNDQVVLSGLARQLVIAELLDERSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGVAFCDLGAIDKDNIPKE
LISEKLVRQHRVIPLWRRGNKLFVGISDPTNHQAITDVQFSTGLTTEAILVEDDKLGDAIEKLFESVTGGLDDLGDVDLE
GLDIESGDAERKEEAIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGMLHEAAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEDAQKQLYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPHCKKEHDVPNETLLHEGFP
EDRIGSFKLYAPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARREGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=332537 ELR50_RS12660 WP_269964080.1 2866460..2868163(+) (pilB) [Pseudomonas citronellolis strain WXP-4]
ATGAACGATCAAGTCGTGCTCTCCGGCCTCGCCCGCCAGTTGGTCATAGCCGAACTGCTGGATGAAAGAAGCGCCCAGCA
GGCCCAGCAGCAGGCCCAGCGCAACAAGCTCTCGCTGGTGACCTACCTAGTCCAGAACAAGCTAGTGAAGAGCCGGCCAC
TCACCGAACTGGCTGCAGATCAGTTCGGTGTCGCCTTCTGCGATTTAGGTGCTATCGACAAAGACAATATCCCCAAGGAA
CTGATCAGCGAAAAACTGGTGCGCCAGCACCGCGTCATCCCCCTCTGGCGACGCGGTAACAAGCTGTTCGTGGGTATTTC
AGACCCTACCAACCATCAAGCCATTACGGATGTACAGTTCAGTACCGGCCTGACCACCGAAGCCATCTTGGTTGAAGACG
ATAAACTCGGTGATGCGATCGAGAAGCTCTTCGAGAGCGTCACCGGTGGGCTAGACGACCTAGGTGACGTCGATCTAGAA
GGCCTGGATATCGAAAGTGGGGATGCCGAGCGAAAGGAGGAAGCCATCGGCGGTGACGCAGATGACGCGCCGGTAGTGCG
CTTCGTCAACAAGATGCTGCTGGATGCCATCAAGGGTGGCTCGTCCGACCTCCACTTTGAGCCCTACGAGAAAACTTACC
GGGTGCGCTTCCGTACCGACGGCATGCTACATGAGGCGGCCAAGCCACCAATCCAACTGGCTAGTCGCATTTCCGCGCGC
CTGAAGGTGATGGCCGGCCTGGACATTTCTGAACGGCGCAAGCCCCAGGATGGGCGCATCAAGATGCGCGTGTCCAAGAG
CAAGTCCATCGACTTCCGCGTTAATACCTTGCCCACACTTTGGGGTGAGAAGATCGTGATGCGGATCCTCGACTCAGCCA
GCGCACAGATGGGCATCGATGCCCTGGGCTACGAGGATGCTCAGAAGCAGCTCTATCTTGAAGCCTTGAAGCAACCGCAA
GGCATGATCCTGGTGACCGGCCCGACAGGCTCCGGCAAGACCGTATCCCTGTATACCGGCCTGAACATTCTCAACACCCC
GGATATCAACATATCCACAGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCTCGCC
AGGGCATGGACTTCGCCCAGGCCTTACGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAGATCCGTGAC
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGATGTCCACCCTACACACCAACAGCGCCGC
GGAAACCCTGACGCGTCTGTTGAACATGGGGGTGGCCTCCTTCAACCTGGCCACCTCCACCAACCTGATCATCGCCCAGC
GCCTGGCGCGCAAGCTCTGCCCGCACTGCAAGAAGGAACACGACGTTCCCAACGAAACCCTGCTCCACGAAGGTTTCCCC
GAGGACAGGATCGGCAGCTTCAAGCTGTACGCCCCCGTAGGGTGTGAAAATTGCAAGAACGGGTACAAGGGCCGCGTGGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATTCAGATCGCGGAGC
AAGCCCGTAGAGAAGGCTTCAATGACCTGCGCACCTCGGGCCTGCTCAAGGCCATGCAAGGCATTACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.333

99.471

0.58
  pilB Acinetobacter baumannii D1279779

57.522

99.647

0.573
  pilB Legionella pneumophila strain ERS1305867

54.85

100

0.549
  pilB Vibrio cholerae strain A1552

50.799

99.295

0.504
  pilB Vibrio campbellii strain DS40M4

49.823

99.824

0.497
  pilF Neisseria gonorrhoeae MS11

49.559

100

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.565

95.767

0.494
  pilF Thermus thermophilus HB27

39.298

100

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.887

93.298

0.372

Multiple sequence alignment