Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   EJF26_RS08420 Genome accession   NZ_CP034442
Coordinates   1649376..1650317 (+) Length   313 a.a.
NCBI ID   WP_000249589.1    Uniprot ID   -
Organism   Streptococcus oralis subsp. dentisani strain F0392     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1644376..1655317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJF26_RS08400 tgt 1644446..1645588 (+) 1143 WP_001285237.1 tRNA guanosine(34) transglycosylase Tgt -
  EJF26_RS08405 - 1645689..1647506 (+) 1818 WP_001220896.1 acyltransferase family protein -
  EJF26_RS08410 nagA 1647647..1648798 (+) 1152 WP_001134436.1 N-acetylglucosamine-6-phosphate deacetylase -
  EJF26_RS08415 - 1648947..1649312 (+) 366 WP_000286403.1 DUF1033 family protein -
  EJF26_RS08420 comGA/cglA/cilD 1649376..1650317 (+) 942 WP_000249589.1 competence type IV pilus ATPase ComGA Machinery gene
  EJF26_RS08425 comGB/cglB 1650265..1651281 (+) 1017 WP_000355376.1 competence type IV pilus assembly protein ComGB Machinery gene
  EJF26_RS08430 comGC/cglC 1651283..1651606 (+) 324 WP_000735797.1 competence type IV pilus major pilin ComGC Machinery gene
  EJF26_RS08435 comGD/cglD 1651569..1652003 (+) 435 WP_001180063.1 competence type IV pilus minor pilin ComGD Machinery gene
  EJF26_RS08440 comGE/cglE 1651966..1652268 (+) 303 WP_000413366.1 competence type IV pilus minor pilin ComGE Machinery gene
  EJF26_RS08445 comGF/cglF 1652231..1652692 (+) 462 WP_000250704.1 competence type IV pilus minor pilin ComGF Machinery gene
  EJF26_RS08450 comGG/cglG 1652670..1653083 (+) 414 WP_000265655.1 competence type IV pilus minor pilin ComGG Machinery gene
  EJF26_RS08455 - 1653116..1653706 (+) 591 WP_000679780.1 class I SAM-dependent methyltransferase -
  EJF26_RS08460 comYH 1653764..1654717 (+) 954 WP_000345148.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35519.40 Da        Isoelectric Point: 5.4197

>NTDB_id=331379 EJF26_RS08420 WP_000249589.1 1649376..1650317(+) (comGA/cglA/cilD) [Streptococcus oralis subsp. dentisani strain F0392]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKVSSLRLSTVGDYRGHESLVIRLLHDEEQELHFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQEDMLQLQLNETIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIIDFANQDYQEHQPTSWNAQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=331379 EJF26_RS08420 WP_000249589.1 1649376..1650317(+) (comGA/cglA/cilD) [Streptococcus oralis subsp. dentisani strain F0392]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGAAAAGAAGGCTCAGGATATCTATTTTATTCCCAAGGA
AAAATCCTATGAGCTTCACATGCGGGTTGGAGACGAACGGTGTCTAGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTAGCGGGTATGAATGTAGGGGAGAAGAGACGTAGTCAGCTGGGCTCTTGCGACTATCAG
CATGGGGAGAAGGTGTCTTCTCTGCGTTTGTCTACCGTAGGAGATTATCGGGGACATGAGAGTTTGGTTATTCGTTTGTT
GCACGATGAGGAGCAGGAACTGCATTTCTGGTTTCAGGATATGAACGAACTGGGTGAGCAGTACCGGCAACGGGGCCTCT
ACCTTTTTGCAGGTCCAGTCGGAAGCGGTAAGACGACTCTGATGCACGAATTAGCTAAGTCTCTCTTTAAGGGGCAGCAG
GTTATGTCCATCGAAGATCCAGTAGAAATCAAGCAGGAAGACATGCTCCAGTTGCAGTTGAATGAGACGATCGGATTGAC
CTATGAAAATCTGATCAAACTGTCTCTCCGACATCGTCCAGATCTCTTGATTATCGGAGAAATTCGGGACAGTGAGACGG
CGCGTGCAGTTGTCAGAGCCAGTTTGACAGGTGCGACAGTTTTTTCAACCATTCATGCCAAGAGTATCCGAGGTGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAACTAGCAGTTGTCCTGCAAGGCGTCTGTTACCAGAGATTAATCGG
GGGAGGAGGAATCATTGACTTTGCAAACCAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGCGCAAATTGATCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

88.818

100

0.888
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

87.54

100

0.875
  comGA/cglA/cilD Streptococcus pneumoniae D39

87.54

100

0.875
  comGA/cglA/cilD Streptococcus pneumoniae R6

87.54

100

0.875
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

87.54

100

0.875
  comYA Streptococcus gordonii str. Challis substr. CH1

79.355

99.042

0.786
  comYA Streptococcus mutans UA159

66.559

99.361

0.661
  comYA Streptococcus mutans UA140

66.559

99.361

0.661
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.968

99.042

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

44.358

82.109

0.364

Multiple sequence alignment