Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   EJF26_RS08425 Genome accession   NZ_CP034442
Coordinates   1650265..1651281 (+) Length   338 a.a.
NCBI ID   WP_000355376.1    Uniprot ID   -
Organism   Streptococcus oralis subsp. dentisani strain F0392     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1645265..1656281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJF26_RS08405 - 1645689..1647506 (+) 1818 WP_001220896.1 acyltransferase family protein -
  EJF26_RS08410 nagA 1647647..1648798 (+) 1152 WP_001134436.1 N-acetylglucosamine-6-phosphate deacetylase -
  EJF26_RS08415 - 1648947..1649312 (+) 366 WP_000286403.1 DUF1033 family protein -
  EJF26_RS08420 comGA/cglA/cilD 1649376..1650317 (+) 942 WP_000249589.1 competence type IV pilus ATPase ComGA Machinery gene
  EJF26_RS08425 comGB/cglB 1650265..1651281 (+) 1017 WP_000355376.1 competence type IV pilus assembly protein ComGB Machinery gene
  EJF26_RS08430 comGC/cglC 1651283..1651606 (+) 324 WP_000735797.1 competence type IV pilus major pilin ComGC Machinery gene
  EJF26_RS08435 comGD/cglD 1651569..1652003 (+) 435 WP_001180063.1 competence type IV pilus minor pilin ComGD Machinery gene
  EJF26_RS08440 comGE/cglE 1651966..1652268 (+) 303 WP_000413366.1 competence type IV pilus minor pilin ComGE Machinery gene
  EJF26_RS08445 comGF/cglF 1652231..1652692 (+) 462 WP_000250704.1 competence type IV pilus minor pilin ComGF Machinery gene
  EJF26_RS08450 comGG/cglG 1652670..1653083 (+) 414 WP_000265655.1 competence type IV pilus minor pilin ComGG Machinery gene
  EJF26_RS08455 - 1653116..1653706 (+) 591 WP_000679780.1 class I SAM-dependent methyltransferase -
  EJF26_RS08460 comYH 1653764..1654717 (+) 954 WP_000345148.1 class I SAM-dependent methyltransferase Machinery gene
  EJF26_RS08465 - 1654767..1655957 (+) 1191 WP_000167801.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38576.75 Da        Isoelectric Point: 9.6717

>NTDB_id=331380 EJF26_RS08425 WP_000355376.1 1650265..1651281(+) (comGB/cglB) [Streptococcus oralis subsp. dentisani strain F0392]
MDISQVFRLKRKKLATAKQKKIITLFNNLFSSGFHLVEIISFLGRSALLEKDYVTQMHQGLSQGKSFSEMMDSLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMMGLRNYLLPQLDSSNIATKIIGNLPQIF
LGLVLVCSLSLLLALTFYKRSSKMRVFSMLARIPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDLAQVLQNGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=331380 EJF26_RS08425 WP_000355376.1 1650265..1651281(+) (comGB/cglB) [Streptococcus oralis subsp. dentisani strain F0392]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAGAAGAAAATCATCACCCTGTTTAA
CAATCTCTTCTCCAGTGGTTTTCATTTGGTGGAAATCATTTCTTTCTTGGGCAGAAGTGCCTTGCTAGAAAAGGACTATG
TGACCCAGATGCACCAAGGCTTGTCCCAGGGGAAATCCTTTTCAGAAATGATGGACAGCTTGGGTTTTTCAAGTGCCATT
GTGACCCAATTATCCCTAGCTGAAGTTCACGGGAATCTTCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATCGAAGTGGCGACCTATCCTTTGATTTTGCTGGGATTTCTCCTGCTAATCATGATGG
GGTTGAGAAACTATTTGCTCCCCCAACTGGACAGTAGCAATATCGCCACCAAAATCATCGGCAATCTGCCACAAATATTT
CTGGGACTAGTGTTGGTTTGCTCTCTATCTTTACTTTTAGCCCTCACTTTTTACAAAAGAAGTTCCAAGATGCGGGTATT
CTCGATGTTAGCACGGATTCCCTTTCTTGGAATCTTTGTCCAGACCTATCTGACGGCCTATTACGCGCGTGAATGGGGCA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAGATCATGCAGGAACAAGGTTCTCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAGTCCTGCAAAATGGTCGCGAATTTTCTCAAACCATAGCGACCTATCCTTTCTTTAAGAAGGA
GTTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAGAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAATCGCACCATGAATTTGGTGCAGCCACTGGTCTTTATCTTTGTGGCCCTGATTATCGTT
TTACTTTATGCGGCAATGCTTATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

93.787

100

0.938
  comGB/cglB Streptococcus mitis NCTC 12261

92.604

100

0.926
  comGB/cglB Streptococcus pneumoniae Rx1

91.124

100

0.911
  comGB/cglB Streptococcus pneumoniae D39

91.124

100

0.911
  comGB/cglB Streptococcus pneumoniae R6

91.124

100

0.911
  comGB/cglB Streptococcus pneumoniae TIGR4

91.124

100

0.911
  comYB Streptococcus gordonii str. Challis substr. CH1

73.214

99.408

0.728
  comYB Streptococcus mutans UA140

58.621

94.379

0.553
  comYB Streptococcus mutans UA159

58.621

94.379

0.553
  comGB Lactococcus lactis subsp. cremoris KW2

52.071

100

0.521

Multiple sequence alignment