Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   FS611_RS20515 Genome accession   NZ_CP042350
Coordinates   4043093..4043857 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain RM10410     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4038093..4048857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FS611_RS20495 (FS611_20490) acpT 4039047..4039634 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  FS611_RS20500 (FS611_20495) nikA 4039745..4041319 (+) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  FS611_RS20505 (FS611_20500) nikB 4041319..4042263 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  FS611_RS20510 (FS611_20505) nikC 4042260..4043093 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  FS611_RS20515 (FS611_20510) amiE 4043093..4043857 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  FS611_RS20520 (FS611_20515) nikE 4043854..4044660 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  FS611_RS20525 (FS611_20520) nikR 4044666..4045067 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=331382 FS611_RS20515 WP_001136229.1 4043093..4043857(+) (amiE) [Escherichia coli strain RM10410]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=331382 FS611_RS20515 WP_001136229.1 4043093..4043857(+) (amiE) [Escherichia coli strain RM10410]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment