Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EIJ81_RS15075 Genome accession   NZ_CP034240
Coordinates   2758023..2758439 (+) Length   138 a.a.
NCBI ID   WP_012551085.1    Uniprot ID   -
Organism   Aliivibrio salmonicida strain VS224     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2753023..2763439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EIJ81_RS15060 (EIJ81_15060) pdhR 2755065..2755835 (-) 771 WP_012551082.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  EIJ81_RS15065 (EIJ81_15065) ampD 2756122..2756673 (-) 552 WP_012551083.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EIJ81_RS15070 (EIJ81_15070) nadC 2756787..2757674 (+) 888 WP_044583329.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EIJ81_RS15075 (EIJ81_15075) pilA 2758023..2758439 (+) 417 WP_012551085.1 pilin Machinery gene
  EIJ81_RS15080 (EIJ81_15080) pilB 2758442..2760133 (+) 1692 WP_012551086.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EIJ81_RS15085 (EIJ81_15085) - 2760161..2761398 (+) 1238 Protein_2628 type II secretion system F family protein -
  EIJ81_RS15090 (EIJ81_15090) pilD 2761507..2762394 (+) 888 WP_012551087.1 A24 family peptidase Machinery gene
  EIJ81_RS15095 (EIJ81_15095) coaE 2762391..2763014 (+) 624 WP_012551088.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14337.54 Da        Isoelectric Point: 6.2210

>NTDB_id=330071 EIJ81_RS15075 WP_012551085.1 2758023..2758439(+) (pilA) [Aliivibrio salmonicida strain VS224]
MNKRQGQKGFTLIELMIVVAIIGVLSAIAIPAYKDYVSKSEAASGLATIKALQTPAELFFQESGTTQPTLAQLGTTAKAN
TLGVITDPLVDSAIVFTFGSDSALNGKTLTLARNSDTGWSCTPSTGVPVMDGCKIVTP

Nucleotide


Download         Length: 417 bp        

>NTDB_id=330071 EIJ81_RS15075 WP_012551085.1 2758023..2758439(+) (pilA) [Aliivibrio salmonicida strain VS224]
ATGAACAAGCGTCAAGGACAGAAAGGCTTCACCTTAATTGAATTAATGATCGTAGTTGCCATCATTGGTGTGTTATCAGC
AATAGCTATTCCTGCTTATAAAGATTATGTTTCAAAAAGTGAGGCTGCGTCTGGGTTAGCAACAATTAAAGCACTGCAAA
CACCGGCAGAATTATTCTTTCAAGAAAGCGGTACTACTCAACCAACATTAGCTCAACTAGGAACAACTGCTAAAGCAAAT
ACCTTAGGGGTAATAACAGATCCTCTTGTTGATAGCGCTATTGTATTTACTTTCGGTTCAGACAGTGCTTTAAATGGCAA
AACATTAACACTAGCAAGAAATTCTGACACTGGTTGGTCTTGTACTCCATCTACAGGTGTTCCTGTTATGGATGGTTGCA
AAATAGTAACACCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-127)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

55.556

97.826

0.543
  pilA Pseudomonas aeruginosa PAK

45.185

97.826

0.442
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.736

93.478

0.428
  pilA Vibrio cholerae C6706

42.857

96.377

0.413
  pilA Vibrio cholerae strain A1552

42.857

96.377

0.413
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.857

96.377

0.413
  pilA2 Legionella pneumophila strain ERS1305867

42.969

92.754

0.399
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.548

89.855

0.391
  pilA Acinetobacter baumannii strain A118

37.063

100

0.384
  pilA2 Legionella pneumophila str. Paris

40.945

92.029

0.377
  pilA/pilA1 Eikenella corrodens VA1

37.778

97.826

0.37

Multiple sequence alignment