Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EIJ81_RS15080 Genome accession   NZ_CP034240
Coordinates   2758442..2760133 (+) Length   563 a.a.
NCBI ID   WP_012551086.1    Uniprot ID   B6ELG1
Organism   Aliivibrio salmonicida strain VS224     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2753442..2765133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EIJ81_RS15060 (EIJ81_15060) pdhR 2755065..2755835 (-) 771 WP_012551082.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  EIJ81_RS15065 (EIJ81_15065) ampD 2756122..2756673 (-) 552 WP_012551083.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EIJ81_RS15070 (EIJ81_15070) nadC 2756787..2757674 (+) 888 WP_044583329.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EIJ81_RS15075 (EIJ81_15075) pilA 2758023..2758439 (+) 417 WP_012551085.1 pilin Machinery gene
  EIJ81_RS15080 (EIJ81_15080) pilB 2758442..2760133 (+) 1692 WP_012551086.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EIJ81_RS15085 (EIJ81_15085) - 2760161..2761398 (+) 1238 Protein_2628 type II secretion system F family protein -
  EIJ81_RS15090 (EIJ81_15090) pilD 2761507..2762394 (+) 888 WP_012551087.1 A24 family peptidase Machinery gene
  EIJ81_RS15095 (EIJ81_15095) coaE 2762391..2763014 (+) 624 WP_012551088.1 dephospho-CoA kinase -
  EIJ81_RS15100 (EIJ81_15100) zapD 2763023..2763769 (+) 747 WP_012551089.1 cell division protein ZapD -
  EIJ81_RS15105 (EIJ81_15105) yacG 2763777..2763986 (+) 210 WP_012551090.1 DNA gyrase inhibitor YacG -
  EIJ81_RS15110 (EIJ81_15110) mutT 2764054..2764455 (-) 402 WP_012551091.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62437.35 Da        Isoelectric Point: 5.3629

>NTDB_id=330072 EIJ81_RS15080 WP_012551086.1 2758442..2760133(+) (pilB) [Aliivibrio salmonicida strain VS224]
MHTNLASILRQAELISLTQENTIIEHVQSGAHTVPSALLAAHIFTPDELISHLSQIFGLDVINVAQYDYATCCQKLGLRE
LITQHQALPIHYDHGLIRLAVSDPSIAQIEDDFRFTTNKQIELVLADEGQLQAAIRRLYGKGLSDSLPSQNKEITQDELA
SLVDISNDEMVFTEDLSQDDAPVTRFINQILLEAVRKGVSDIHFEPYEETYRVRFRCDGILVENSSPSSHLSRHLSARLK
IMAKLDIAERRLPQDGRIKLKLNSEVSIDMRVSSLPTLWGEKIVLRLLDSSAASLDIDKLGYSDAQKELYLTALKRPQGM
ILMTGPTGSGKTVSLYSGLNILNTTEKNISTAEDPVEINLNGINQVQVNPKIGFTFAYALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETVVRLGNMGIENFNLASSLTLIIAQRLARRLCPHCKAIDPLTPEIREEYGIDLL
GTVFQAHTEGCNECTHGYSGRIGIYEVMPFTRELSIAILKGATVRQIEQLAIKQGMDNLATSGIKRLEDGTTSLQELTRV
LYF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=330072 EIJ81_RS15080 WP_012551086.1 2758442..2760133(+) (pilB) [Aliivibrio salmonicida strain VS224]
ATGCACACCAACCTAGCTTCAATCTTGCGTCAAGCTGAACTCATCAGCTTGACGCAAGAAAATACCATTATCGAACACGT
TCAATCAGGGGCACACACTGTGCCTTCTGCGCTTTTAGCTGCTCATATTTTTACACCTGATGAATTAATCAGTCATCTTT
CTCAAATTTTTGGTTTGGATGTCATTAATGTCGCTCAATATGATTACGCCACGTGCTGTCAAAAATTAGGACTAAGAGAG
CTCATAACCCAACATCAAGCATTGCCGATTCATTATGATCACGGCTTGATTCGACTCGCAGTGAGTGATCCAAGTATTGC
TCAAATTGAGGATGATTTTCGTTTCACTACCAACAAACAAATTGAATTAGTGTTAGCTGACGAAGGACAGCTTCAAGCGG
CCATTCGGCGTCTTTATGGGAAAGGGTTATCGGATTCACTGCCATCACAAAATAAAGAAATCACCCAAGATGAGCTCGCA
TCACTGGTTGATATATCAAATGATGAAATGGTATTCACAGAAGATCTCAGCCAAGACGACGCCCCGGTTACCCGTTTTAT
AAACCAAATATTACTAGAGGCCGTTCGAAAAGGCGTTTCTGATATTCACTTTGAACCTTATGAAGAAACTTATCGTGTGC
GTTTTCGTTGTGATGGGATTTTAGTAGAGAACAGTAGTCCTTCTTCTCATTTAAGTCGTCATCTTTCAGCCCGCTTAAAA
ATCATGGCAAAACTTGATATCGCAGAGCGACGATTACCACAAGACGGACGCATCAAACTTAAACTGAACAGCGAAGTCTC
CATTGATATGCGCGTGTCCTCTCTTCCTACGTTATGGGGAGAGAAGATTGTTCTTCGATTATTAGACAGCAGCGCGGCCA
GTTTGGATATCGACAAACTCGGCTACAGTGACGCCCAAAAAGAGTTATACCTAACGGCATTAAAACGCCCTCAAGGCATG
ATCTTAATGACGGGCCCGACCGGCAGTGGCAAAACCGTATCTCTTTACTCTGGCCTAAACATTTTAAATACGACAGAAAA
AAACATCTCCACCGCAGAAGATCCTGTCGAAATAAATCTGAATGGCATTAACCAAGTTCAAGTAAACCCTAAAATTGGAT
TTACCTTTGCTTACGCCTTGCGATCATTTCTTCGTCAAGATCCCGATATTGTAATGGTCGGAGAGATCCGAGATCTAGAA
ACAGCCGAAATCGCAGTAAAAGCCGCACAAACTGGGCACTTAGTTCTTTCAACACTTCATACCAACTCAGCGGCAGAAAC
AGTGGTACGTTTAGGAAATATGGGGATCGAGAATTTTAACCTTGCCTCCTCTCTTACTCTTATCATTGCTCAACGATTAG
CAAGAAGGCTTTGCCCTCATTGCAAAGCTATCGATCCATTAACCCCAGAGATCAGAGAAGAATATGGTATTGATTTATTA
GGCACTGTTTTTCAAGCGCACACTGAAGGCTGTAATGAATGTACGCATGGCTATTCAGGGCGAATTGGTATTTACGAAGT
GATGCCCTTTACCCGAGAGCTTTCCATTGCGATTTTAAAAGGCGCAACGGTAAGACAGATTGAACAACTTGCTATCAAGC
AAGGCATGGATAATTTAGCCACATCAGGAATTAAACGCCTCGAAGATGGTACAACCAGCTTACAAGAATTAACTCGTGTT
CTGTATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B6ELG1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

67.851

100

0.679
  pilB Vibrio cholerae strain A1552

67.73

100

0.679
  pilB Vibrio campbellii strain DS40M4

66.844

100

0.67
  pilB Legionella pneumophila strain ERS1305867

49.436

94.494

0.467
  pilB Acinetobacter baumannii D1279779

49.333

93.25

0.46
  pilB Acinetobacter baylyi ADP1

49.606

90.231

0.448
  pilF Neisseria gonorrhoeae MS11

43.363

100

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.299

99.822

0.362

Multiple sequence alignment