Detailed information NTG0330073

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EIJ81_RS15090
• Genome accession: NZ_CP034240
• Coordinates: 2761507..2762394 (+)
• Length: 295 a.a.
• NCBI ID: WP_012551087.1
• Uniprot ID: B6ELG3
• Organism: Aliivibrio salmonicida strain VS224
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2756507..2767394

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EIJ81_RS15070 (EIJ81_15070)	nadC	2756787..2757674 (+)	888	WP_044583329.1	carboxylating nicotinate-nucleotide diphosphorylase	-
EIJ81_RS15075 (EIJ81_15075)	pilA	2758023..2758439 (+)	417	WP_012551085.1	pilin	Machinery gene
EIJ81_RS15080 (EIJ81_15080)	pilB	2758442..2760133 (+)	1692	WP_012551086.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EIJ81_RS15085 (EIJ81_15085)	-	2760161..2761398 (+)	1238	Protein_2628	type II secretion system F family protein	-
EIJ81_RS15090 (EIJ81_15090)	pilD	2761507..2762394 (+)	888	WP_012551087.1	A24 family peptidase	Machinery gene
EIJ81_RS15095 (EIJ81_15095)	coaE	2762391..2763014 (+)	624	WP_012551088.1	dephospho-CoA kinase	-
EIJ81_RS15100 (EIJ81_15100)	zapD	2763023..2763769 (+)	747	WP_012551089.1	cell division protein ZapD	-
EIJ81_RS15105 (EIJ81_15105)	yacG	2763777..2763986 (+)	210	WP_012551090.1	DNA gyrase inhibitor YacG	-
EIJ81_RS15110 (EIJ81_15110)	mutT	2764054..2764455 (-)	402	WP_012551091.1	8-oxo-dGTP diphosphatase MutT	-
EIJ81_RS15115 (EIJ81_15115)	secA	2764555..2767278 (-)	2724	WP_012551092.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 295 a.a.
• Molecular weight: 33225.60 Da
• Isoelectric Point: 7.7758

>NTDB_id=330073 EIJ81_RS15090 WP_012551087.1 2761507..2762394(+) (pilD) [Aliivibrio salmonicida strain VS224]
MAAFEYYPWLFPLFATLFGLLVGSFLNVVIYRLPIMMEKEWKKECTECFPDLKSTPKTADIEEKFNLSVPRSRCPKCNTQ
IKFYDNIPVISWLLLRGKCRQCSNPISFRYPAIELLSGAMCFGVSYLLPFSYFAIAAVLFTLTLIALTFIDIDTMLLPDQ
ITLPLLWTGLYLSLVGWSSVSLMDSVIGAMAGYLILWSIYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPLIVLLSS
IVGAIIGIAMLRAQKNGYDKAIPFGPYLAIAGWICLLWGQEISQWYFTSILGFPV

Nucleotide

• Length: 888 bp

>NTDB_id=330073 EIJ81_RS15090 WP_012551087.1 2761507..2762394(+) (pilD) [Aliivibrio salmonicida strain VS224]
ATGGCTGCTTTTGAATACTACCCTTGGCTCTTTCCTCTCTTTGCTACTTTATTTGGCCTACTCGTCGGTAGCTTTTTAAA
TGTGGTCATCTACCGTTTACCCATAATGATGGAAAAAGAATGGAAAAAAGAATGCACTGAGTGCTTTCCTGATTTGAAAT
CCACACCAAAAACAGCGGATATAGAAGAAAAATTCAATCTAAGTGTTCCTCGTTCACGCTGCCCTAAATGCAATACTCAA
ATAAAATTTTACGATAACATTCCCGTTATTAGCTGGTTGTTGTTGCGTGGAAAATGCCGTCAATGCAGTAATCCTATTAG
CTTCCGTTATCCTGCCATTGAGCTTCTGTCAGGCGCAATGTGTTTTGGCGTCTCTTACTTATTACCATTTTCTTATTTTG
CTATTGCTGCCGTTCTTTTTACTCTAACGCTTATTGCATTAACCTTTATTGATATCGATACCATGTTACTCCCCGATCAA
ATAACGCTGCCATTACTGTGGACTGGCCTATACTTATCCTTAGTCGGTTGGAGTTCAGTCAGCTTAATGGATTCGGTCAT
TGGAGCCATGGCTGGTTATTTAATATTATGGTCCATTTATTGGGCATTTAAATTATTAACCGGTAAAGAAGGCATGGGTT
ATGGGGACTTTAAATTATTAGCCGCACTTGGTGCTTGGCTTGGTTGGCAACAATTGCCATTAATCGTTTTATTGTCTTCT
ATTGTTGGTGCCATCATTGGCATTGCAATGTTAAGGGCACAAAAAAATGGGTATGACAAAGCCATTCCATTTGGTCCTTA
TCTCGCGATTGCAGGTTGGATATGTTTATTGTGGGGCCAAGAAATTAGCCAATGGTACTTCACCTCAATTCTAGGGTTTC
CCGTATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (139-248)

3D structure

• AlphaFold DB: B6ELG3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	67.918	99.322	0.675
pilD	Vibrio cholerae strain A1552	64.746	100	0.647
pilD	Acinetobacter nosocomialis M2	50.365	92.881	0.468
pilD	Acinetobacter baumannii D1279779	50	92.881	0.464
pilD	Neisseria gonorrhoeae MS11	49.064	90.508	0.444