Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EGV93_RS25950 Genome accession   NZ_CP033686
Coordinates   5390996..5391448 (-) Length   150 a.a.
NCBI ID   WP_031636800.1    Uniprot ID   P17837
Organism   Pseudomonas aeruginosa strain H25883     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5385996..5396448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGV93_RS25930 (EGV93_25850) ampD 5386691..5387257 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EGV93_RS25935 (EGV93_25855) - 5387408..5389663 (-) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  EGV93_RS25940 (EGV93_25860) nadC 5389949..5390797 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EGV93_RS25950 (EGV93_25870) pilA 5390996..5391448 (-) 453 WP_031636800.1 pilin Machinery gene
  EGV93_RS25955 (EGV93_25875) pilB 5391680..5393380 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EGV93_RS25960 (EGV93_25880) pilC 5393384..5394604 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  EGV93_RS25965 (EGV93_25885) pilD 5394608..5395480 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EGV93_RS25970 (EGV93_25890) coaE 5395477..5396088 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  EGV93_RS25975 (EGV93_25895) yacG 5396085..5396285 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15743.11 Da        Isoelectric Point: 8.4742

>NTDB_id=324805 EGV93_RS25950 WP_031636800.1 5390996..5391448(-) (pilA) [Pseudomonas aeruginosa strain H25883]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVTIKDTGDGTVKFTFATGQSSPKNAGKEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=324805 EGV93_RS25950 WP_031636800.1 5390996..5391448(-) (pilA) [Pseudomonas aeruginosa strain H25883]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAAGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P17837

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

66.447

100

0.673
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45

100

0.48
  pilA Vibrio cholerae strain A1552

45

100

0.48
  pilA Vibrio cholerae C6706

45

100

0.48
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46

100

0.46
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

98.667

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.333

0.413
  comP Acinetobacter baylyi ADP1

41.333

100

0.413
  pilA/pilA1 Eikenella corrodens VA1

39.865

98.667

0.393
  pilA2 Legionella pneumophila str. Paris

38.255

99.333

0.38
  pilA2 Legionella pneumophila strain ERS1305867

38.255

99.333

0.38

Multiple sequence alignment