Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EGV93_RS25960 Genome accession   NZ_CP033686
Coordinates   5393384..5394604 (+) Length   406 a.a.
NCBI ID   WP_009315898.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H25883     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5388384..5399604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGV93_RS25940 (EGV93_25860) nadC 5389949..5390797 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EGV93_RS25950 (EGV93_25870) pilA 5390996..5391448 (-) 453 WP_031636800.1 pilin Machinery gene
  EGV93_RS25955 (EGV93_25875) pilB 5391680..5393380 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EGV93_RS25960 (EGV93_25880) pilC 5393384..5394604 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  EGV93_RS25965 (EGV93_25885) pilD 5394608..5395480 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EGV93_RS25970 (EGV93_25890) coaE 5395477..5396088 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  EGV93_RS25975 (EGV93_25895) yacG 5396085..5396285 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  EGV93_RS25980 (EGV93_25900) - 5396322..5396531 (-) 210 WP_003094660.1 hypothetical protein -
  EGV93_RS25985 (EGV93_25905) - 5396637..5397326 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  EGV93_RS25990 (EGV93_25910) - 5397323..5397793 (-) 471 WP_003094664.1 hypothetical protein -
  EGV93_RS25995 (EGV93_25915) - 5397790..5398215 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  EGV93_RS26000 (EGV93_25920) - 5398348..5398977 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  EGV93_RS26005 (EGV93_25925) - 5398974..5399423 (+) 450 WP_023100233.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44288.04 Da        Isoelectric Point: 9.8784

>NTDB_id=324807 EGV93_RS25960 WP_009315898.1 5393384..5394604(+) (pilC) [Pseudomonas aeruginosa strain H25883]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGAIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=324807 EGV93_RS25960 WP_009315898.1 5393384..5394604(+) (pilC) [Pseudomonas aeruginosa strain H25883]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTGAAACCCATGGACATTGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGTTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCACAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGTGTGGGGGCGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCAGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGCCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baumannii D1279779

60.891

99.507

0.606
  pilC Acinetobacter baylyi ADP1

60.591

100

0.606
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.562

97.783

0.406
  pilC Thermus thermophilus HB27

37.157

98.768

0.367

Multiple sequence alignment