Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   EBA23_RS08265 Genome accession   NZ_CP033190
Coordinates   1616264..1616689 (+) Length   141 a.a.
NCBI ID   WP_011408068.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzae strain PXO404     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1605477..1619053 1616264..1616689 within 0


Gene organization within MGE regions


Location: 1605477..1619053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBA23_RS08190 - 1605626..1605925 (+) 300 WP_125168747.1 hypothetical protein -
  EBA23_RS08195 - 1605922..1606251 (+) 330 WP_125168748.1 hypothetical protein -
  EBA23_RS08200 (EBA23_08155) - 1606328..1607761 (+) 1434 Protein_1471 RHS repeat-associated core domain-containing protein -
  EBA23_RS08205 (EBA23_08160) - 1607767..1608135 (+) 369 WP_133260527.1 DUF6869 domain-containing protein -
  EBA23_RS08210 (EBA23_08165) - 1608305..1609273 (-) 969 WP_011409545.1 IS5-like element ISXo1 family transposase -
  EBA23_RS08215 (EBA23_08170) - 1609369..1609536 (+) 168 Protein_1474 IS701 family transposase -
  EBA23_RS08225 (EBA23_08180) - 1609654..1609875 (+) 222 Protein_1475 IS4 family transposase -
  EBA23_RS08230 (EBA23_08185) - 1609965..1610921 (+) 957 WP_041182041.1 IS30-like element IS1112a family transposase -
  EBA23_RS08235 (EBA23_08190) - 1610905..1611207 (+) 303 WP_158645227.1 hypothetical protein -
  EBA23_RS08245 (EBA23_08200) - 1611768..1613087 (-) 1320 WP_133260528.1 IS701-like element ISXo15 family transposase -
  EBA23_RS08250 (EBA23_08205) coaE 1613158..1613766 (-) 609 WP_011258357.1 dephospho-CoA kinase -
  EBA23_RS08255 (EBA23_08210) - 1613780..1614643 (-) 864 WP_011258358.1 A24 family peptidase -
  EBA23_RS08260 (EBA23_08215) pilC 1614650..1615909 (-) 1260 WP_011408067.1 type II secretion system F family protein Machinery gene
  EBA23_RS08265 (EBA23_08220) pilA2 1616264..1616689 (+) 426 WP_011408068.1 pilin Machinery gene
  EBA23_RS08270 (EBA23_08225) - 1616824..1618413 (+) 1590 WP_011408069.1 phosphoethanolamine transferase -
  EBA23_RS08275 (EBA23_08230) - 1618424..1619053 (+) 630 WP_011408070.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14442.76 Da        Isoelectric Point: 9.0090

>NTDB_id=322291 EBA23_RS08265 WP_011408068.1 1616264..1616689(+) (pilA2) [Xanthomonas oryzae pv. oryzae strain PXO404]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVTETASSLGGVANVTLANSGYSFPGATK
YVSNVTIADGTGLVTVTSTVPNAAGTILLTPKDVGGGQLKWTCSSAILTKYLPAECRSSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=322291 EBA23_RS08265 WP_011408068.1 1616264..1616689(+) (pilA2) [Xanthomonas oryzae pv. oryzae strain PXO404]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCTGCCATCGCGCT
GCCGGCTTATCAGGACTACACCACTCGTGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCGCCTGCCAAACTTGCGGTAA
CGGAGACTGCATCGTCGCTCGGCGGTGTTGCTAATGTTACTCTCGCGAATTCGGGCTACTCATTCCCGGGTGCCACGAAG
TATGTCAGCAACGTCACCATCGCCGATGGTACGGGCCTAGTCACTGTAACTTCTACCGTGCCGAACGCAGCCGGTACCAT
CCTGCTGACCCCGAAGGATGTCGGTGGCGGCCAGCTGAAGTGGACCTGCTCTTCCGCCATCCTCACCAAGTACCTGCCGG
CGGAGTGCCGTAGCTCGGGCACCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.471

96.454

0.496
  pilA2 Legionella pneumophila strain ERS1305867

51.471

96.454

0.496
  pilA Ralstonia pseudosolanacearum GMI1000

41.818

100

0.489
  comP Acinetobacter baylyi ADP1

43.537

100

0.454
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.054

100

0.447
  pilE Neisseria gonorrhoeae strain FA1090

37.278

100

0.447
  pilE Neisseria gonorrhoeae MS11

37.5

100

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.446

98.582

0.418
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.222

95.745

0.404
  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.397
  pilA Acinetobacter baumannii strain A118

38.298

100

0.383
  pilA Pseudomonas aeruginosa PAK

36

100

0.383
  pilA Vibrio parahaemolyticus RIMD 2210633

40.157

90.071

0.362

Multiple sequence alignment