Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EAG14_RS05105 Genome accession   NZ_CP033069
Coordinates   1127629..1128762 (-) Length   377 a.a.
NCBI ID   WP_099656360.1    Uniprot ID   -
Organism   Acidovorax sp. 1608163     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1122629..1133762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAG14_RS05080 (EAG14_05080) - 1123027..1123458 (+) 432 WP_099656364.1 Hsp20/alpha crystallin family protein -
  EAG14_RS05085 (EAG14_05085) - 1123651..1124346 (-) 696 WP_371414400.1 ABC transporter ATP-binding protein -
  EAG14_RS05090 (EAG14_05090) miaA 1124388..1125407 (-) 1020 WP_121730306.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  EAG14_RS05095 (EAG14_05095) - 1125397..1126650 (-) 1254 WP_121728253.1 multidrug effflux MFS transporter -
  EAG14_RS05100 (EAG14_05100) - 1126719..1127558 (-) 840 WP_121728254.1 alpha/beta hydrolase -
  EAG14_RS05105 (EAG14_05105) pilU 1127629..1128762 (-) 1134 WP_099656360.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EAG14_RS05110 (EAG14_05110) gdhA 1128934..1130277 (-) 1344 WP_121730307.1 NADP-specific glutamate dehydrogenase -
  EAG14_RS05115 (EAG14_05115) - 1130545..1131756 (+) 1212 WP_233194988.1 glycerophosphodiester phosphodiesterase -
  EAG14_RS05120 (EAG14_05120) - 1131854..1132843 (-) 990 WP_240456944.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41512.82 Da        Isoelectric Point: 6.0878

>NTDB_id=321142 EAG14_RS05105 WP_099656360.1 1127629..1128762(-) (pilU) [Acidovorax sp. 1608163]
MEIFNFLNLMVEKAGSDLFFSVGAPVNVKIEGITHPLKMPALLPGQVKQLAYSIMNERQISEFEAKQEMNLSISAENLGR
FRVNVFVQRGETGMVVRYIKNKIPPLAALGLPPVLEKLVMLKRGLVLVTGATGSGKSTTLASMLNFRNENTTGHILTIED
PLEFLHSHKLSVVDQREVGIDTQSYEEALKNALREAPDVIMIGEIRDRNTMKQAIAYAETGHLCLSTLHANNANQAMERV
INFFPEDAHHQLLMDLSLNLAGVVSQRLIPGLQEKLVPAVEVMLTSPYIADLIGKGEFSGIKEAMGHSTEIGMCTFDQAL
YKLYTEGRIALDEALHNADSRTDLALRVRLAAGVNTSDAGDLTIEPTSPQHRASQHF

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=321142 EAG14_RS05105 WP_099656360.1 1127629..1128762(-) (pilU) [Acidovorax sp. 1608163]
ATGGAAATCTTCAACTTCCTGAACCTCATGGTCGAGAAGGCGGGCTCGGACCTGTTCTTCAGCGTGGGCGCGCCCGTTAA
CGTGAAGATCGAGGGCATCACCCACCCGCTCAAGATGCCCGCGCTGTTGCCGGGCCAGGTCAAGCAGCTGGCGTACTCGA
TCATGAACGAGCGCCAGATCAGCGAGTTCGAGGCCAAGCAGGAGATGAACCTGTCCATCTCGGCCGAGAACCTGGGGCGC
TTTCGGGTGAATGTGTTTGTGCAGCGCGGCGAGACCGGCATGGTGGTGCGCTATATCAAGAACAAAATCCCGCCGCTGGC
CGCACTGGGCCTGCCCCCGGTGCTGGAGAAGCTGGTCATGCTCAAGCGCGGGCTGGTGCTGGTGACGGGCGCCACGGGCT
CGGGCAAGTCCACCACGCTGGCGTCCATGCTCAACTTCCGCAACGAGAACACCACGGGCCACATCCTCACCATCGAAGAC
CCGCTGGAGTTTCTGCACTCGCACAAGCTCTCGGTGGTCGACCAGCGCGAGGTCGGCATTGACACCCAGTCGTACGAAGA
GGCCCTCAAGAACGCTCTGCGCGAGGCGCCCGACGTGATCATGATTGGCGAGATCCGCGACCGCAACACCATGAAGCAGG
CCATTGCCTATGCCGAGACCGGGCACCTGTGTCTGTCGACCCTACACGCCAACAACGCCAACCAGGCCATGGAGCGGGTG
ATCAACTTCTTCCCGGAAGACGCACACCACCAACTGCTGATGGACCTGAGCCTGAACCTGGCGGGCGTGGTGTCGCAGCG
GCTCATCCCCGGCCTGCAAGAAAAGCTGGTGCCTGCGGTCGAGGTCATGCTCACCTCGCCCTACATTGCCGACCTGATCG
GCAAGGGCGAGTTCTCGGGCATCAAGGAGGCCATGGGCCACAGCACCGAAATTGGCATGTGCACCTTTGACCAGGCGCTG
TACAAGCTCTACACCGAAGGCCGCATTGCGCTGGATGAGGCCCTGCACAACGCCGACTCGCGCACCGACCTGGCGCTGCG
CGTGCGCCTGGCGGCAGGGGTAAATACCTCGGATGCCGGGGATTTGACCATTGAGCCCACATCGCCCCAGCACCGGGCGT
CACAGCATTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.703

98.143

0.517
  pilU Acinetobacter baylyi ADP1

49.86

94.695

0.472
  pilU Vibrio cholerae strain A1552

50

93.899

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.541

98.143

0.398
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Pseudomonas aeruginosa PAK

40.237

89.655

0.361
  pilT Pseudomonas stutzeri DSM 10701

40.237

89.655

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.329

0.361
  pilT Vibrio cholerae strain A1552

40.841

88.329

0.361
  pilT Acinetobacter baumannii D1279779

41.212

87.533

0.361
  pilT Acinetobacter nosocomialis M2

41.212

87.533

0.361
  pilT Acinetobacter baumannii strain A118

41.212

87.533

0.361

Multiple sequence alignment