Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA/pilAI   Type   Machinery gene
Locus tag   TMZ1T_RS18890 Genome accession   NC_011662
Coordinates   4291911..4292312 (+) Length   133 a.a.
NCBI ID   WP_012586208.1    Uniprot ID   C4KD70
Organism   Thauera aminoaromatica     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4287620..4304144 4291911..4292312 within 0


Gene organization within MGE regions


Location: 4287620..4304144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TMZ1T_RS21690 - 4287620..4288120 (-) 501 WP_261789087.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  TMZ1T_RS18860 - 4288144..4288524 (+) 381 WP_261789088.1 putative toxin-antitoxin system toxin component, PIN family -
  TMZ1T_RS18865 (Tmz1t_3892) - 4288511..4290196 (-) 1686 WP_012586206.1 IS1634 family transposase -
  TMZ1T_RS18870 - 4290305..4290508 (+) 204 WP_187283014.1 hypothetical protein -
  TMZ1T_RS18875 (Tmz1t_3893) - 4290579..4290833 (-) 255 WP_012586207.1 Txe/YoeB family addiction module toxin -
  TMZ1T_RS18880 (Tmz1t_3894) - 4290823..4291089 (-) 267 WP_004361138.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
  TMZ1T_RS18885 - 4291210..4291365 (+) 156 WP_144312187.1 hypothetical protein -
  TMZ1T_RS20745 (Tmz1t_3895) - 4291382..4291690 (+) 309 WP_261789205.1 type II toxin-antitoxin system RelE/ParE family toxin -
  TMZ1T_RS18890 (Tmz1t_3896) pilA/pilAI 4291911..4292312 (+) 402 WP_012586208.1 pilin Machinery gene
  TMZ1T_RS18895 (Tmz1t_3897) - 4292407..4294353 (+) 1947 WP_012586209.1 lipopolysaccharide assembly protein LapB -
  TMZ1T_RS18900 (Tmz1t_3898) - 4294350..4295207 (+) 858 WP_012586210.1 glycosyltransferase family 2 protein -
  TMZ1T_RS18905 (Tmz1t_3899) - 4295379..4296482 (+) 1104 WP_222702378.1 glycosyltransferase -
  TMZ1T_RS18910 (Tmz1t_3900) - 4296508..4298214 (+) 1707 WP_012586212.1 ABC transporter ATP-binding protein -
  TMZ1T_RS20750 (Tmz1t_3901) - 4298292..4299311 (+) 1020 WP_083768713.1 glycosyltransferase -
  TMZ1T_RS21210 (Tmz1t_3902) - 4299330..4301372 (+) 2043 WP_144312188.1 HAD family hydrolase -
  TMZ1T_RS18915 (Tmz1t_3903) glf 4301380..4302489 (+) 1110 WP_012586215.1 UDP-galactopyranose mutase -
  TMZ1T_RS18920 (Tmz1t_3904) - 4302505..4303629 (+) 1125 WP_012586216.1 glycosyltransferase family 4 protein -
  TMZ1T_RS18925 (Tmz1t_3905) - 4303764..4304144 (-) 381 WP_012586217.1 type II toxin-antitoxin system HigA family antitoxin -

Sequence


Protein


Download         Length: 133 a.a.        Molecular weight: 13593.77 Da        Isoelectric Point: 8.8611

>NTDB_id=32024 TMZ1T_RS18890 WP_012586208.1 4291911..4292312(+) (pilA/pilAI) [Thauera aminoaromatica]
MKKMQQGFTLIELMIVVAIIGILAAVALPAYSDYQAKAKLTAGLAEISAGKTAFEELRNNNVAVTAAADIGLQATTGNCA
ITATATTIVCTLANAPSQVNGKTITWTRTASTGAWSCATSAPAKYAPKTCPGV

Nucleotide


Download         Length: 402 bp        

>NTDB_id=32024 TMZ1T_RS18890 WP_012586208.1 4291911..4292312(+) (pilA/pilAI) [Thauera aminoaromatica]
ATGAAAAAGATGCAACAAGGCTTCACCCTGATCGAACTGATGATCGTCGTGGCGATCATCGGCATCCTGGCTGCGGTGGC
GCTGCCGGCGTATTCGGATTACCAGGCCAAGGCGAAACTTACCGCAGGCCTTGCGGAGATCTCGGCAGGTAAGACTGCAT
TTGAGGAACTCCGTAACAACAACGTTGCGGTTACCGCCGCGGCCGATATTGGCCTCCAAGCCACGACCGGGAACTGCGCC
ATTACTGCGACCGCCACAACCATCGTTTGCACTCTGGCGAATGCGCCTTCGCAGGTCAATGGCAAGACGATCACCTGGAC
TCGAACCGCAAGCACTGGCGCGTGGAGTTGCGCAACCAGCGCCCCGGCCAAGTACGCGCCGAAGACCTGCCCGGGCGTCT
AA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB C4KD70

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.529

100

0.496

  pilA2 Legionella pneumophila str. Paris

42.759

100

0.466

  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.203

100

0.459

  pilA2 Legionella pneumophila strain ERS1305867

42.254

100

0.451

  pilA Acinetobacter baumannii strain A118

43.704

100

0.444

  comP Acinetobacter baylyi ADP1

39.726

100

0.436

  pilA Ralstonia pseudosolanacearum GMI1000

34.969

100

0.429

  pilA Vibrio parahaemolyticus RIMD 2210633

43.411

96.992

0.421

  pilA Pseudomonas aeruginosa PAK

38.095

100

0.421

  pilA Vibrio cholerae C6706

37.162

100

0.414

  pilA Vibrio cholerae strain A1552

37.162

100

0.414

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.162

100

0.414

  pilA/pilA1 Eikenella corrodens VA1

39.13

100

0.406


Multiple sequence alignment