Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SWP_RS16355 Genome accession   NC_011566
Coordinates   3885691..3886803 (-) Length   370 a.a.
NCBI ID   WP_020913703.1    Uniprot ID   B8CS68
Organism   Shewanella piezotolerans WP3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3880691..3891803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SWP_RS16335 (swp_3667) ppnN 3880868..3882220 (+) 1353 WP_020913699.1 nucleotide 5'-monophosphate nucleosidase PpnN -
  SWP_RS16340 (swp_3668) xni 3882217..3882984 (+) 768 WP_020913700.1 flap endonuclease Xni -
  SWP_RS16345 (swp_3669) - 3883091..3883480 (+) 390 WP_020913701.1 DUF3192 domain-containing protein -
  SWP_RS16350 (swp_3670) hemH 3884263..3885276 (-) 1014 WP_020913702.1 ferrochelatase -
  SWP_RS16355 (swp_3671) pilU 3885691..3886803 (-) 1113 WP_020913703.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SWP_RS16360 (swp_3672) pilT 3886817..3887854 (-) 1038 WP_044556009.1 type IV pilus twitching motility protein PilT Machinery gene
  SWP_RS16365 (swp_3673) - 3887890..3888627 (+) 738 WP_044556010.1 YggS family pyridoxal phosphate-dependent enzyme -
  SWP_RS16370 (swp_3674) proC 3888747..3889565 (+) 819 WP_020913706.1 pyrroline-5-carboxylate reductase -
  SWP_RS16375 (swp_3675) - 3889597..3890145 (+) 549 WP_020913707.1 YggT family protein -
  SWP_RS16380 (swp_3676) yggU 3890145..3890432 (+) 288 WP_143711207.1 DUF167 family protein YggU -
  SWP_RS16385 (swp_3677) - 3890516..3890950 (+) 435 WP_020913709.1 DUF4426 domain-containing protein -
  SWP_RS16390 (swp_3678) rdgB 3891173..3891772 (+) 600 WP_020913710.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40988.85 Da        Isoelectric Point: 5.8984

>NTDB_id=31809 SWP_RS16355 WP_020913703.1 3885691..3886803(-) (pilU) [Shewanella piezotolerans WP3]
MDVRPFLKVMIDRKASDLFITAGFPPSAKIDGELRPLSENAFNPAQALEFVESLMTDVQKVEFHESRECNFAFAAKDLGR
FRVSAFWQREAPGCVMRRIETQIPEVDDLMLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGTGRRAAIEVLINTPRVASLIAKNELHSLKETMSKSNEQGMQTFDQA
LLKLYSEGEISYADALHHADSPNDLRLMIKLQSSDTTSTSSMDGVTLNLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=31809 SWP_RS16355 WP_020913703.1 3885691..3886803(-) (pilU) [Shewanella piezotolerans WP3]
ATGGATGTTCGTCCTTTTTTAAAGGTCATGATAGATCGTAAAGCATCAGATCTGTTTATTACAGCGGGTTTCCCCCCGAG
TGCAAAGATAGACGGTGAACTACGCCCTCTTAGTGAGAATGCATTTAACCCTGCACAAGCATTGGAGTTTGTTGAGTCAC
TTATGACCGACGTGCAAAAAGTGGAGTTTCATGAATCTCGTGAGTGTAACTTCGCATTTGCGGCTAAAGATTTAGGCCGG
TTTCGTGTCAGTGCTTTTTGGCAAAGGGAGGCACCAGGTTGCGTAATGCGCCGTATTGAAACTCAAATCCCAGAAGTTGA
TGATTTAATGCTGCCGCCTATCTTGAAAGATTTAGTAATGAGTAAGCGTGGCTTAGTCATTATGGTGGGTGGCACAGGAA
CGGGTAAATCGACATCGCTGGCAGCACTGGTTGGCTACCGAAATGCACATGCGCGTGGCCATATTCTGACCATCGAAGAT
CCTGTCGAATTTGTTCATGATCACCGCCAAAGCATTATTACCCAACGGGAAGTGGGCATTGATACTGAATCTTTTGATGC
GGCGCTTAAAAGTTCACTGCGTCAAGCGCCGGATGTGATCCTCATTGGTGAGATCCGTAGCCAAGAGACAATGGAATTTG
CGCTGTCTTTTGCTGAAACGGGTCATCTTTGTATGGCGACACTACACGCCAACAACGCTAACCAAGCACTTGACCGTATT
ATGCACCTAGTACCAGAGAGTAAGCATCATCAGCTGTTATTTGATTTGTCGCTTAACTTACGTGGGATTGTGGCGCAGCA
ACTGGTACCTAAAAGTGATGGAACCGGGCGTAGAGCTGCAATTGAAGTACTCATTAATACGCCGCGAGTGGCGAGCTTGA
TTGCTAAAAACGAACTGCATTCGTTAAAAGAAACCATGTCTAAATCTAACGAGCAGGGGATGCAAACGTTCGATCAGGCA
TTGTTGAAGTTATACAGTGAAGGCGAGATTAGCTACGCTGATGCATTGCACCATGCTGATTCACCCAATGACTTAAGGCT
AATGATTAAGCTGCAAAGCTCAGATACCACGAGTACATCATCAATGGATGGCGTCACACTTAATCTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8CS68

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.838

100

0.6
  pilU Vibrio cholerae strain A1552

57.568

100

0.576
  pilU Acinetobacter baylyi ADP1

60.227

95.135

0.573
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

93.243

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Legionella pneumophila strain Lp02

40.785

89.459

0.365
  pilT Legionella pneumophila strain ERS1305867

40.785

89.459

0.365
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362

Multiple sequence alignment