Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   D5S12_RS04525 Genome accession   NZ_CP032459
Coordinates   988982..989407 (+) Length   141 a.a.
NCBI ID   WP_044323946.1    Uniprot ID   -
Organism   Pseudomonas syringae strain CAS02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 983982..994407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5S12_RS04500 (D5S12_04820) yacG 984142..984351 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  D5S12_RS04505 (D5S12_04825) coaE 984348..984971 (-) 624 WP_058397527.1 dephospho-CoA kinase -
  D5S12_RS04510 (D5S12_04830) pilD 984968..985840 (-) 873 WP_187600008.1 prepilin peptidase Machinery gene
  D5S12_RS04515 (D5S12_04835) pilC 985842..987059 (-) 1218 WP_058397525.1 type II secretion system F family protein Machinery gene
  D5S12_RS04520 (D5S12_04840) pilB 987062..988756 (-) 1695 WP_058397524.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D5S12_RS04525 (D5S12_04845) pilA2 988982..989407 (+) 426 WP_044323946.1 pilin Machinery gene
  D5S12_RS04535 (D5S12_04855) - 989767..990312 (-) 546 WP_003371273.1 DUF1523 family protein -
  D5S12_RS04540 (D5S12_04860) - 990432..991430 (+) 999 WP_187600010.1 ATP-grasp domain-containing protein -
  D5S12_RS04545 (D5S12_04865) - 991485..992624 (+) 1140 WP_060708665.1 trypsin-like peptidase domain-containing protein -
  D5S12_RS04550 (D5S12_04870) - 992691..993824 (+) 1134 WP_187600015.1 phosphoribosyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14450.49 Da        Isoelectric Point: 8.4743

>NTDB_id=316022 D5S12_RS04525 WP_044323946.1 988982..989407(+) (pilA2) [Pseudomonas syringae strain CAS02]
MRSQKGFTLIELMIVVAIVGILAAVALPAYQDYTVRAQVAELIGLTDGAKVAVTENYQSTGAFPASNQAAGYGGATGRYT
TSVVIGANGLITATADAVNSNPAIRGLTITLTPAVAAAGNNVVTWTCGGTIDQRFRPTSCR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=316022 D5S12_RS04525 WP_044323946.1 988982..989407(+) (pilA2) [Pseudomonas syringae strain CAS02]
ATGAGATCACAAAAAGGTTTTACCCTTATTGAATTGATGATTGTTGTTGCAATCGTCGGTATCTTGGCGGCGGTCGCACT
GCCTGCATATCAGGATTATACGGTTCGAGCCCAAGTGGCTGAGCTCATCGGTCTGACTGATGGTGCCAAGGTCGCAGTTA
CTGAAAATTATCAATCAACAGGTGCATTTCCTGCTTCTAACCAAGCTGCTGGCTATGGCGGAGCAACTGGTCGCTATACG
ACTTCTGTAGTGATTGGTGCTAATGGATTGATTACCGCTACAGCTGATGCTGTAAACTCGAACCCTGCAATTCGTGGTCT
GACCATTACGCTAACTCCGGCTGTGGCAGCCGCAGGTAATAATGTCGTTACATGGACATGCGGCGGTACTATTGACCAGC
GTTTCCGTCCGACGAGCTGCCGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

55.245

100

0.56
  pilA2 Legionella pneumophila strain ERS1305867

55.245

100

0.56
  pilE Neisseria gonorrhoeae MS11

44.654

100

0.504
  pilE Neisseria gonorrhoeae strain FA1090

44.304

100

0.496
  pilA/pilA1 Eikenella corrodens VA1

43.59

100

0.482
  comP Acinetobacter baylyi ADP1

41.611

100

0.44
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.559

100

0.411
  pilA Acinetobacter baumannii strain A118

38

100

0.404
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.437

100

0.397
  pilA Pseudomonas aeruginosa PAK

35

100

0.397
  pilA Haemophilus influenzae 86-028NP

36.986

100

0.383

Multiple sequence alignment