Detailed information NTG0316019

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: D5S12_RS04510
• Genome accession: NZ_CP032459
• Coordinates: 984968..985840 (-)
• Length: 290 a.a.
• NCBI ID: WP_187600008.1
• Uniprot ID: -
• Organism: Pseudomonas syringae strain CAS02
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 979968..990840

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D5S12_RS04475 (D5S12_04795)	-	980753..982051 (-)	1299	WP_003392712.1	NAD(P)/FAD-dependent oxidoreductase	-
D5S12_RS26395	-	982147..982383 (-)	237	WP_316933231.1	DUF3094 family protein	-
D5S12_RS04485 (D5S12_04805)	-	982268..982801 (-)	534	WP_003430737.1	MOSC domain-containing protein	-
D5S12_RS04490 (D5S12_04810)	-	982798..983427 (-)	630	WP_003345130.1	DUF1780 domain-containing protein	-
D5S12_RS04495 (D5S12_04815)	-	983555..984016 (+)	462	WP_058397529.1	hypothetical protein	-
D5S12_RS04500 (D5S12_04820)	yacG	984142..984351 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
D5S12_RS04505 (D5S12_04825)	coaE	984348..984971 (-)	624	WP_058397527.1	dephospho-CoA kinase	-
D5S12_RS04510 (D5S12_04830)	pilD	984968..985840 (-)	873	WP_187600008.1	prepilin peptidase	Machinery gene
D5S12_RS04515 (D5S12_04835)	pilC	985842..987059 (-)	1218	WP_058397525.1	type II secretion system F family protein	Machinery gene
D5S12_RS04520 (D5S12_04840)	pilB	987062..988756 (-)	1695	WP_058397524.1	type IV-A pilus assembly ATPase PilB	Machinery gene
D5S12_RS04525 (D5S12_04845)	pilA2	988982..989407 (+)	426	WP_044323946.1	pilin	Machinery gene
D5S12_RS04535 (D5S12_04855)	-	989767..990312 (-)	546	WP_003371273.1	DUF1523 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32035.21 Da
• Isoelectric Point: 8.1512

>NTDB_id=316019 D5S12_RS04510 WP_187600008.1 984968..985840(-) (pilD) [Pseudomonas syringae strain CAS02]
MPLLDLLASSLLAFVITCCILGLIIGSFLNVVVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISQRYPLVELTCAVLSAYVAWHFGFGWQAAAILVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNSFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=316019 D5S12_RS04510 WP_187600008.1 984968..985840(-) (pilD) [Pseudomonas syringae strain CAS02]
ATGCCCCTCCTCGACCTCTTGGCCAGCTCACTGCTGGCCTTCGTCATTACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCCTCCCGATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAACTGCTCGGCC
TGCCCGCAGAACCCGATCAACCCGTCTTCAATCTGAACCGCCCCCGCTCCAGCTGCCCGCATTGCGCACACAAAATCCGC
CCATGGGAAAACCTGCCGGTGATCAGCTATCTGCTGCTGCGCGGTAAATGTTCGCAGTGCAAAGCGCCGATCAGCCAACG
TTACCCGCTGGTCGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTGGCCTGGCATTTCGGCTTCGGCTGGCAGGCAGCGG
CGATACTGGTGTTGAGCTGGGGCCTGCTGGCGATGAGTCTGATCGACGCCGACCACCAGCTGTTACCTGACTCTCTGGTG
CTGCCACTTCTGTGGCTGGGCCTCATCGTCAACTCTTTCGGGCTGTTCACCTCGCTGAACGATGCGCTTTGGGGCGCTGT
GGCCGGTTATCTGGCGCTGTGGTCGGTGTTTTGGCTGTTCAAGCTCGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCGATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCGTTAACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTTGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCTATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.38	94.483	0.514
pilD	Vibrio campbellii strain DS40M4	53.846	94.138	0.507
pilD	Acinetobacter baumannii D1279779	52.518	95.862	0.503
pilD	Acinetobacter nosocomialis M2	51.799	95.862	0.497
pilD	Neisseria gonorrhoeae MS11	52.174	87.241	0.455