Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   D5S12_RS04515 Genome accession   NZ_CP032459
Coordinates   985842..987059 (-) Length   405 a.a.
NCBI ID   WP_058397525.1    Uniprot ID   -
Organism   Pseudomonas syringae strain CAS02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 980842..992059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5S12_RS26395 - 982147..982383 (-) 237 WP_316933231.1 DUF3094 family protein -
  D5S12_RS04485 (D5S12_04805) - 982268..982801 (-) 534 WP_003430737.1 MOSC domain-containing protein -
  D5S12_RS04490 (D5S12_04810) - 982798..983427 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  D5S12_RS04495 (D5S12_04815) - 983555..984016 (+) 462 WP_058397529.1 hypothetical protein -
  D5S12_RS04500 (D5S12_04820) yacG 984142..984351 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  D5S12_RS04505 (D5S12_04825) coaE 984348..984971 (-) 624 WP_058397527.1 dephospho-CoA kinase -
  D5S12_RS04510 (D5S12_04830) pilD 984968..985840 (-) 873 WP_187600008.1 prepilin peptidase Machinery gene
  D5S12_RS04515 (D5S12_04835) pilC 985842..987059 (-) 1218 WP_058397525.1 type II secretion system F family protein Machinery gene
  D5S12_RS04520 (D5S12_04840) pilB 987062..988756 (-) 1695 WP_058397524.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D5S12_RS04525 (D5S12_04845) pilA2 988982..989407 (+) 426 WP_044323946.1 pilin Machinery gene
  D5S12_RS04535 (D5S12_04855) - 989767..990312 (-) 546 WP_003371273.1 DUF1523 family protein -
  D5S12_RS04540 (D5S12_04860) - 990432..991430 (+) 999 WP_187600010.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44091.04 Da        Isoelectric Point: 10.1744

>NTDB_id=316020 D5S12_RS04515 WP_058397525.1 985842..987059(-) (pilC) [Pseudomonas syringae strain CAS02]
MASKVVKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVNSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAVIVSGILLIKVVPQFQSVFAGFGAELPTFTLMVIGLSVFIQEWWLAIIGIFFASIFMFK
KSYKKSQKFRDSLDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVIFKNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMAILGVVVGGLVIAMYLPIFK
LGNAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=316020 D5S12_RS04515 WP_058397525.1 985842..987059(-) (pilC) [Pseudomonas syringae strain CAS02]
ATGGCCAGCAAGGTAGTCAAAGTTATCGTTTACACGTGGGAAGGTGTTGACAAGAAAGGCACTAAAACCAGTGGCGAACT
GAGCGGCCATAACCTGGCGCTCGTAAAGGCCCAGTTACGCAAGCAAGGCATCAATCCTACCAAGGTGCGTAAGAAGTCTG
CGTCGATATTCGGTAAGGGCAAAAAAATCAAGCCATTGGACATCGCGTTTTTTTCTCGGCAGATGGCAACGATGATGAAG
GCTGGCGTACCATTGCTGCAGTCGTTCGACATAATCAGCGAGGGTGCAGAGAACCCGAACATGCGAACATTGGTAAACTC
TCTGAAGCAGGAAGTTTCAGCAGGTAACAGCTTTGCGACGGCTTTGCGGCAAAAGCCCGAATATTTCGACGACCTCTTTT
GCAATCTTGTGGATGCTGGTGAGCAAGCCGGCGCACTTGAGAGTTTGCTGGACCGAGTTGCGAGCTACAAAGAGAAGACC
GAAAAGCTCAAGGCAAAAATCAAAAAGGCTATGACCTATCCCGCTGCAGTATTAATAGTAGCAGTTATAGTTTCAGGAAT
CCTGCTTATCAAAGTTGTACCACAATTTCAATCAGTATTCGCTGGATTCGGAGCAGAGCTGCCGACCTTTACTTTGATGG
TTATTGGACTATCCGTTTTCATACAGGAGTGGTGGCTAGCGATCATCGGGATTTTTTTTGCTTCAATATTTATGTTCAAA
AAATCATATAAAAAGTCTCAAAAATTTCGCGACAGTCTGGATCGTTTTTTATTAAAAGTGCCGATTATAGGACCGCTAAT
TTTTAAATCTTCAGTAGCCCGCTACGCACGCACACTAGCGACGACGTTTGCAGCTGGCGTCCCGTTGGTAGAGGCGCTGG
ATTCAGTCGCAGGAGCGACCGGCAATGTCATATTCAAGAACGCAGTCAACAAGGTCAAGCAGGACGTCTCGACCGGTATG
CAGCTTAACTTCTCAATGCGCTCCACCGGGGTTTTTCCTAGCTTAGCAATTCAGATGACTGCTATAGGTGAGGAGTCCGG
CGCACTTGACAGTATGCTCGATAAAGTTGCGACCTATTACGAGGATGAGGTAGATAACATGGTAGATAGCCTGACCAGTC
TCATGGAGCCTATGATAATGGCAATCCTAGGCGTGGTTGTCGGAGGTTTAGTAATTGCTATGTACTTACCGATTTTTAAA
TTAGGAAACGCAGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.086

100

0.731
  pilC Acinetobacter baylyi ADP1

59.559

100

0.6
  pilC Acinetobacter baumannii D1279779

60.957

98.025

0.598
  pilC Legionella pneumophila strain ERS1305867

52.381

98.519

0.516
  pilG Neisseria gonorrhoeae MS11

44.472

98.272

0.437
  pilG Neisseria meningitidis 44/76-A

44.221

98.272

0.435
  pilC Vibrio campbellii strain DS40M4

43.257

97.037

0.42
  pilC Vibrio cholerae strain A1552

42.145

99.012

0.417
  pilC Thermus thermophilus HB27

37.656

99.012

0.373

Multiple sequence alignment