Detailed information    

experimental Experimentally validated

Overview

Name   clpX   Type   Unclear
Locus tag   Cj0275 Genome accession   NC_002163
Coordinates   251784..253037 (+) Length   417 a.a.
NCBI ID   YP_002343717.1    Uniprot ID   Q9PIM0
Organism   Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819     
Function   require for natural transformation   
Unclear

Function

This showed the ΔclpP mutant of C. jejuni to be practically untransformable, while mutants lacking either the ClpX or the ClpA ATPases showed approximately 93 and 80% reduced transformation, respectively, when compared to the wild-type (wt) strain


Genomic Context

Location: 246784..258037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cj0269c ilvE 247656..248570 (-) 915 YP_002343711.1 branched-chain amino acid aminotransferase -
  Cj0270 - 248747..248953 (+) 207 YP_002343712.1 tautomerase family protein -
  Cj0271 - 248950..249405 (+) 456 YP_002343713.1 bacterioferritin comigratory protein -
  Cj0272 - 249405..250496 (+) 1092 YP_002343714.1 hypothetical protein -
  Cj0273 fabZ 250590..251030 (+) 441 YP_002343715.1 3-hydroxyacyl-ACP dehydratase -
  Cj0274 lpxA 251030..251821 (+) 792 YP_002343716.1 acyl-[acyl-carrier-protein]--UDP-N- acetylglucosamine O-acyltransferase -
  Cj0275 clpX 251784..253037 (+) 1254 YP_002343717.1 ATP-dependent protease ATP-binding subunit ClpX Unclear
  Cj0276 mreB 253049..254089 (+) 1041 YP_002343718.1 rod shape-determining protein MreB -
  Cj0277 mreC 254079..254828 (+) 750 YP_002343719.1 rod shape-determining protein MreC -

Sequence

Protein


Download         Length: 417 a.a.        Molecular weight: 47049.19 Da        Isoelectric Point: 4.9071

>NTDB_id=1475 Cj0275 YP_002343717.1 251784..253037(+) (clpX) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
MEFLSIGVKIMPRKCSFCNEVENPQRRILANENDDAFICEYCVEGAYSIIYGEEKEFKEPKQSHNTEFKDITPKELKAYL
DRYVIGQDRAKKVFSVGVYNHYKRLFKAELQDDDTELFKSNILLVGPTGSGKTLLAQTLAKFLDVPIAICDATSLTEAGY
VGEDVENILTRLLQAADGDVQRAQKGIVFIDEIDKIARMSENRSITRDVSGEGVQQALLKIIEGSLVNIPPRGGRKHPNQ
EFIQIDTSNILFVCGGAFDGLETILKRKLGDKVVGFFDDAKEENKALLEKIEPDDLVHFGLIPELIGRLHVIASLNELNE
EDMVRILTEPKNAIIKQYQKLFAIDGVNLKFEEDALRAIAQLALERKTGARGLRSIIEEMMVDLMFELPEYKDYDIVITK
EVVKDNAKALLIKRKIS

Nucleotide


Download         Length: 1254 bp        

>NTDB_id=1475 Cj0275 YP_002343717.1 251784..253037(+) (clpX) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
GTGGAATTCCTGTCTATAGGGGTAAAAATAATGCCTAGAAAATGTAGTTTTTGCAATGAAGTGGAGAATCCACAAAGAAG
AATTTTAGCAAATGAAAATGATGATGCTTTTATTTGTGAATATTGCGTTGAAGGTGCTTATAGTATTATTTATGGAGAAG
AAAAAGAATTTAAAGAACCTAAACAAAGTCATAATACAGAATTTAAAGATATCACTCCAAAAGAATTAAAAGCTTATTTA
GATCGTTATGTCATAGGACAAGATAGAGCTAAAAAAGTTTTTAGTGTTGGAGTATACAATCATTATAAAAGACTTTTTAA
AGCTGAACTTCAAGATGATGATACAGAGCTTTTTAAGTCAAATATTTTACTTGTTGGGCCAACAGGAAGTGGAAAAACTT
TATTGGCGCAAACTTTAGCTAAATTTTTAGATGTACCTATAGCAATTTGTGATGCAACTTCTTTAACTGAAGCAGGATAT
GTAGGTGAGGATGTGGAAAATATTTTAACACGTCTTTTACAAGCTGCAGATGGAGATGTGCAAAGAGCTCAAAAAGGTAT
AGTTTTTATTGATGAGATTGATAAAATTGCCAGAATGAGTGAAAATCGTTCTATTACTCGTGATGTAAGTGGAGAGGGTG
TGCAACAAGCTTTACTTAAGATTATCGAAGGAAGTTTGGTAAATATTCCACCAAGGGGGGGGAGAAAACATCCAAATCAA
GAGTTTATACAAATTGACACTTCAAATATTTTATTTGTTTGTGGTGGAGCTTTTGATGGTTTGGAAACTATCTTAAAAAG
AAAACTTGGAGATAAAGTTGTAGGTTTTTTTGATGATGCAAAAGAAGAAAATAAAGCTTTACTTGAGAAAATTGAACCTG
ATGATTTGGTTCATTTTGGTTTGATTCCTGAGCTTATAGGTCGTTTGCATGTGATTGCATCTTTAAATGAGCTTAATGAA
GAAGATATGGTAAGAATTTTAACTGAACCTAAAAATGCCATTATTAAGCAATATCAAAAGCTTTTTGCTATTGATGGGGT
AAATTTAAAATTTGAAGAAGATGCTCTAAGAGCTATCGCACAACTTGCATTGGAGAGAAAAACAGGGGCTAGGGGGCTTA
GAAGTATTATAGAAGAAATGATGGTAGATTTAATGTTTGAGTTACCAGAGTATAAAGACTATGATATTGTAATAACCAAA
GAAGTTGTTAAAGACAATGCAAAGGCATTATTAATAAAAAGAAAAATAAGTTAA

Domains

Predicted by InterproScan.

AAA domain (Cdc48 subfamily) (AAA_2)

(119-312)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(318-389)

ClpX C4-type zinc finger (zf-C4_ClpX)

(14-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9PIM0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpX Streptococcus mutans UA159

59.102

97.805

0.578

Multiple sequence alignment    



References

[1] Christina S Vegge et al. (2012) Natural transformation of Campylobacter jejuni occurs beyond limits of growth. PloS One 7(9):e45467. [PMID: 23049803]