Detailed information    

experimental Experimentally validated

Overview

Name   clpX   Type   Regulator
Locus tag   SMU_RS04375 Genome accession   NC_004350
Coordinates   898059..899291 (+) Length   410 a.a.
NCBI ID   WP_002262816.1    Uniprot ID   A0AAX1K4W9
Organism   Streptococcus mutans UA159     
Function   require for competence development   
Competence regulation

Function

If the transformation is carried out at the early growth phase, both ClpP protease and ClpX ATPase are needed for competence. In contrast, we found that both ClpC and ClpE negatively affect competence. We also found that if the transformation is carried out at the mid-logarithmic growth phase ClpX is still required for competence, but ClpP negatively affects competence.


Genomic Context

Location: 893059..904291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS04355 (SMU.943c) - 894131..895306 (-) 1176 WP_002266471.1 hydroxymethylglutaryl-CoA synthase -
  SMU_RS04360 (SMU.944) - 895514..896353 (+) 840 WP_002263663.1 thymidylate synthase -
  SMU_RS04365 (SMU.946) pnuC 896470..897249 (+) 780 WP_002262813.1 nicotinamide riboside transporter PnuC -
  SMU_RS04370 (SMU.947) - 897334..897846 (+) 513 WP_002262814.1 dihydrofolate reductase -
  SMU_RS09915 (SMU.948) - 897864..898034 (+) 171 WP_002262815.1 hypothetical protein -
  SMU_RS04375 (SMU.949) clpX 898059..899291 (+) 1233 WP_002262816.1 ATP-dependent Clp protease ATP-binding subunit ClpX Regulator
  SMU_RS04380 (SMU.950) yihA 899307..899900 (+) 594 WP_002262817.1 ribosome biogenesis GTP-binding protein YihA/YsxC -
  SMU_RS04385 (SMU.951) - 900229..901605 (+) 1377 WP_002262818.1 amino acid permease -
  SMU_RS04390 (SMU.952) mmuM 901615..902565 (+) 951 WP_002262819.1 homocysteine S-methyltransferase -
  SMU_RS04395 (SMU.953c) - 902699..903961 (-) 1263 WP_002263804.1 PLP-dependent aminotransferase family protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45181.65 Da        Isoelectric Point: 4.4495

>NTDB_id=379 SMU_RS04375 WP_002262816.1 898059..899291(+) (clpX) [Streptococcus mutans UA159]
MAGNRTNDVTVHCSFCGKNQDEVKKIIAGNGVFICNECVELSQEIIREELAEEVLADLSEVPKPKELLAILDSYVIGQDR
AKRALAVAVYNHYKRISFTESQDDQDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVENI
LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVASVPPQGGRKHPQQEMIQIDTK
NILFIVGGAFDGIEDIVKQRLGEKIIGFGQNNKKIDDQSSYMQEIISEDIQKFGLIPEFIGRLPVLAALEQLTVDDLVKI
LTEPKNALVKQYQTLLSYDGVELEFDQEALQAIAQKAIERKTGARGLRSIIEETMLDLMFEIPSQEDVTCVRITKKAVEG
TDKPILETAS

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=379 SMU_RS04375 WP_002262816.1 898059..899291(+) (clpX) [Streptococcus mutans UA159]
ATGGCTGGAAATAGAACCAATGATGTCACAGTGCATTGTTCATTCTGTGGCAAAAATCAAGATGAAGTTAAAAAAATTAT
TGCAGGTAATGGCGTTTTTATTTGCAATGAATGTGTTGAATTATCACAAGAAATTATCCGTGAAGAACTAGCAGAAGAGG
TATTGGCTGATCTTTCAGAAGTTCCAAAACCAAAAGAATTGTTGGCAATACTAGACAGTTATGTTATCGGTCAGGACCGT
GCCAAACGTGCTTTAGCAGTTGCTGTCTATAATCATTATAAGCGTATCAGTTTTACTGAGAGCCAAGACGACCAAGATGT
TGACTTACAAAAATCAAATATTCTTATGATTGGACCGACAGGTTCTGGAAAAACTTTCTTAGCACAGACTTTGGCTAAGA
GTCTCAATGTGCCCTTTGCTATTGCTGATGCAACAGCCTTGACTGAAGCGGGTTATGTAGGTGAAGATGTTGAAAACATT
TTGCTTAAATTGATTCAGGCAGCTGATTATAATGTTGAACGCGCGGAGCGTGGCATCATTTATGTTGATGAAATTGATAA
GATTGCTAAAAAAGGCGAAAATGTTTCCATCACACGTGATGTATCTGGTGAAGGTGTCCAACAAGCTCTGCTTAAGATTA
TTGAAGGAACAGTAGCTAGTGTGCCACCACAAGGTGGTCGGAAACATCCCCAACAGGAAATGATTCAAATTGATACGAAA
AACATCCTCTTCATAGTTGGTGGCGCTTTTGATGGTATTGAAGATATCGTTAAACAGCGTTTGGGTGAGAAAATTATTGG
CTTTGGTCAAAATAATAAGAAGATTGATGATCAATCTTCTTATATGCAAGAAATTATTTCAGAAGACATTCAAAAATTTG
GTCTTATTCCAGAGTTTATTGGTCGTTTGCCAGTTTTAGCTGCGCTTGAGCAATTAACGGTTGATGATTTGGTTAAGATT
TTGACAGAACCTAAAAATGCTTTAGTTAAACAATATCAGACGCTTTTATCTTATGATGGTGTTGAGCTTGAGTTTGATCA
AGAGGCGCTTCAAGCGATTGCTCAAAAGGCGATTGAACGTAAGACAGGCGCACGCGGTCTTCGCTCTATTATTGAAGAAA
CGATGCTGGATCTGATGTTTGAAATTCCGAGTCAAGAGGATGTCACTTGCGTTCGCATTACTAAGAAAGCGGTAGAAGGG
ACCGACAAACCTATTCTTGAGACAGCTTCCTAA

Domains

Predicted by InterproScan.

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(312-388)

AAA domain (Cdc48 subfamily) (AAA_2)

(110-305)

ClpX C4-type zinc finger (zf-C4_ClpX)

(12-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpX Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

59.102

97.805

0.578

Multiple sequence alignment    



References

[1] Satya D Pandey et al. (2022) Clp ATPases differentially affect natural competence development in Streptococcus mutans. MicrobiologyOpen 11(3):e1288. [PMID: 35765180]