Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NGK_RS02185 Genome accession   NC_011035
Coordinates   413370..414500 (+) Length   376 a.a.
NCBI ID   WP_003704392.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae NCCP11945     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408370..419500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGK_RS15295 (NGK_0498) - 408816..408965 (+) 150 WP_003687747.1 hypothetical protein -
  NGK_RS14910 - 409189..409296 (+) 108 Protein_406 IS5/IS1182 family transposase -
  NGK_RS02170 (NGK_0500) lepB 409401..410420 (-) 1020 WP_003706641.1 signal peptidase I -
  NGK_RS02175 (NGK_0501) lepA 410566..412359 (-) 1794 WP_003687751.1 translation elongation factor 4 -
  NGK_RS02180 (NGK_0502) - 412501..413202 (-) 702 WP_003687752.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  NGK_RS02185 (NGK_0503) pilU 413370..414500 (+) 1131 WP_003704392.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NGK_RS02190 (NGK_0504) - 414534..415511 (+) 978 WP_003687755.1 DNA polymerase III subunit delta' -
  NGK_RS02195 (NGK_0505) - 415515..415865 (+) 351 WP_003687757.1 PilZ domain-containing protein -
  NGK_RS02200 (NGK_0506) - 415870..416649 (+) 780 WP_003694907.1 TatD family hydrolase -
  NGK_RS02205 - 416675..416890 (+) 216 WP_003690799.1 DUF333 domain-containing protein -
  NGK_RS02210 (NGK_0508) grxD 416991..417302 (+) 312 WP_003687762.1 Grx4 family monothiol glutaredoxin -
  NGK_RS02220 (NGK_0510) upp 417408..418034 (+) 627 WP_003690802.1 uracil phosphoribosyltransferase -
  NGK_RS02225 (NGK_0511) - 418057..418377 (+) 321 WP_003690805.1 DUF2322 family protein -
  NGK_RS02230 (NGK_0512) - 418546..419187 (+) 642 Protein_418 IS1595 family transposase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.32 Da        Isoelectric Point: 6.2891

>NTDB_id=31215 NGK_RS02185 WP_003704392.1 413370..414500(+) (pilU) [Neisseria gonorrhoeae NCCP11945]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=31215 NGK_RS02185 WP_003704392.1 413370..414500(+) (pilU) [Neisseria gonorrhoeae NCCP11945]
ATGTTTACCGATGAAAATATGACTGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCTATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGTGCGACGGCGTTGGTATTCCG
CGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGCCTTGAAGGATGTTGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTCGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGATCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATTACCCAGCGCGA
GGTCGGCGTGGACACGGAAAACTGGATGGCGGCGTTGAAAAATACGCTGCGTCAGGCGCCGGATGTGATCCTTATCGGCG
AAATCCGCGACCGTGAAACAATGGACTACGCCATCGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTAC
TACTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGAGATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCCGCACATGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCAGGGCACAAAGTTCCGACCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.659

93.617

0.559
  pilU Acinetobacter baylyi ADP1

57.726

91.223

0.527
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Acinetobacter baylyi ADP1

42.727

87.766

0.375
  pilT Pseudomonas aeruginosa PAK

42.378

87.234

0.37
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment