Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   BEN71_RS02215 Genome accession   NZ_CP031716
Coordinates   328226..328657 (+) Length   143 a.a.
NCBI ID   WP_068973835.1    Uniprot ID   -
Organism   Acinetobacter wuhouensis strain WCHA60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323226..333657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN71_RS02190 (BEN71_02190) - 324228..324632 (+) 405 WP_068973838.1 ribosome-binding factor A -
  BEN71_RS02195 (BEN71_02195) - 324718..325539 (+) 822 WP_068973837.1 M48 family metallopeptidase -
  BEN71_RS02200 (BEN71_02200) - 325637..326227 (+) 591 WP_068973923.1 LemA family protein -
  BEN71_RS02205 (BEN71_02205) - 326321..327451 (+) 1131 WP_068973922.1 YgcG family protein -
  BEN71_RS02210 (BEN71_02210) - 327445..328005 (+) 561 WP_068973836.1 TPM domain-containing protein -
  BEN71_RS02215 (BEN71_02215) pilE 328226..328657 (+) 432 WP_068973835.1 pilin Machinery gene
  BEN71_RS02220 (BEN71_02220) - 328718..330628 (+) 1911 WP_068973834.1 hypothetical protein -
  BEN71_RS02225 (BEN71_02225) - 330639..331487 (+) 849 WP_068973833.1 glycosyltransferase family 2 protein -
  BEN71_RS02230 (BEN71_02230) - 331580..333214 (+) 1635 WP_406565257.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14782.95 Da        Isoelectric Point: 9.2979

>NTDB_id=310674 BEN71_RS02215 WP_068973835.1 328226..328657(+) (pilE) [Acinetobacter wuhouensis strain WCHA60]
MKSVQKGFTLIELMIVVAIIGILAAIAIPAYQDYIARSQMTEAMNLAGGQKGAVTEYYSNKGTFPTNNTDAGVAAAASIK
GKYVDNVAISGGKITATLKSSDVSEGIKSATLVLSPTTSAGSVEWKCTGSTASTAKYYPTACR

Nucleotide


Download         Length: 432 bp        

>NTDB_id=310674 BEN71_RS02215 WP_068973835.1 328226..328657(+) (pilE) [Acinetobacter wuhouensis strain WCHA60]
ATGAAATCAGTTCAAAAGGGTTTCACCCTTATCGAATTAATGATCGTTGTTGCGATTATCGGTATTCTTGCTGCAATTGC
GATTCCTGCTTATCAAGATTATATTGCGCGTTCACAAATGACTGAAGCAATGAATCTTGCTGGTGGTCAAAAAGGTGCTG
TTACTGAATATTATTCGAATAAAGGCACTTTCCCAACCAATAACACGGATGCAGGTGTTGCAGCAGCAGCAAGTATTAAA
GGTAAATATGTAGATAATGTTGCAATTAGCGGTGGTAAAATCACAGCAACATTAAAGAGTTCAGATGTCTCTGAAGGTAT
TAAAAGTGCAACTCTCGTGCTTAGTCCAACTACTTCAGCTGGTTCAGTTGAGTGGAAATGTACAGGTTCAACAGCTTCAA
CAGCAAAATATTATCCAACTGCTTGTCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

51.572

100

0.573
  pilE Neisseria gonorrhoeae strain FA1090

50.633

100

0.559
  pilA2 Legionella pneumophila str. Paris

47.518

98.601

0.469
  pilA2 Legionella pneumophila strain ERS1305867

46.809

98.601

0.462
  pilA Ralstonia pseudosolanacearum GMI1000

38.596

100

0.462
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.043

100

0.448
  pilA Acinetobacter baumannii strain A118

43.151

100

0.441
  comP Acinetobacter baylyi ADP1

42.282

100

0.441
  pilA/pilA1 Eikenella corrodens VA1

38.365

100

0.427
  pilA Acinetobacter nosocomialis M2

51.724

81.119

0.42
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.549

99.301

0.413
  pilA Pseudomonas aeruginosa PAK

39.456

100

0.406
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.806

100

0.371

Multiple sequence alignment