Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   BRN32_RS08315 Genome accession   NZ_CP031461
Coordinates   1612645..1613070 (+) Length   141 a.a.
NCBI ID   WP_011408068.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzae strain OS198     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1596625..1626015 1612645..1613070 within 0


Gene organization within MGE regions


Location: 1596625..1626015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRN32_RS24655 - 1600407..1600895 (+) 489 WP_128415435.1 DUF6714 family protein -
  BRN32_RS08250 (BRN32_08250) - 1600947..1601915 (+) 969 WP_151420406.1 IS5-like element ISXo1 family transposase -
  BRN32_RS24660 - 1602064..1602363 (+) 300 WP_125168747.1 hypothetical protein -
  BRN32_RS24665 - 1602360..1602689 (+) 330 WP_125168748.1 hypothetical protein -
  BRN32_RS08255 (BRN32_08255) - 1602691..1604199 (+) 1509 WP_075240223.1 RHS repeat-associated core domain-containing protein -
  BRN32_RS08260 (BRN32_08260) - 1604160..1604573 (+) 414 WP_258533483.1 DUF6869 domain-containing protein -
  BRN32_RS25160 (BRN32_08265) - 1604647..1604808 (+) 162 Protein_1495 IS701 family transposase -
  BRN32_RS08270 (BRN32_08270) - 1604895..1605743 (-) 849 Protein_1496 IS5-like element ISXo1 family transposase -
  BRN32_RS24670 - 1605833..1606030 (+) 198 WP_158645226.1 hypothetical protein -
  BRN32_RS08280 (BRN32_08280) - 1606055..1606276 (+) 222 Protein_1498 IS4 family transposase -
  BRN32_RS08285 (BRN32_08285) - 1606367..1607325 (+) 959 Protein_1499 IS30 family transposase -
  BRN32_RS08290 (BRN32_08290) - 1607309..1607611 (+) 303 WP_158645227.1 hypothetical protein -
  BRN32_RS24675 - 1607608..1608138 (+) 531 WP_264484154.1 DUF6714 family protein -
  BRN32_RS08295 (BRN32_08295) - 1608140..1609468 (-) 1329 Protein_1502 IS701 family transposase -
  BRN32_RS08300 (BRN32_08300) coaE 1609539..1610147 (-) 609 WP_011258357.1 dephospho-CoA kinase -
  BRN32_RS08305 (BRN32_08305) - 1610161..1611024 (-) 864 WP_011258358.1 A24 family peptidase -
  BRN32_RS08310 (BRN32_08310) pilC 1611031..1612290 (-) 1260 WP_011408067.1 type II secretion system F family protein Machinery gene
  BRN32_RS08315 (BRN32_08315) pilA2 1612645..1613070 (+) 426 WP_011408068.1 pilin Machinery gene
  BRN32_RS08320 (BRN32_08320) - 1613205..1614794 (+) 1590 WP_075243392.1 phosphoethanolamine transferase -
  BRN32_RS08325 (BRN32_08325) - 1614805..1615434 (+) 630 WP_011408070.1 hypothetical protein -
  BRN32_RS08330 (BRN32_08330) - 1615404..1616930 (+) 1527 WP_075243393.1 hypothetical protein -
  BRN32_RS08335 (BRN32_08335) pilB 1616965..1618698 (+) 1734 WP_075243394.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BRN32_RS08340 (BRN32_08340) - 1618707..1618967 (-) 261 WP_343182274.1 Fic family protein -
  BRN32_RS24680 - 1619055..1619216 (-) 162 WP_153296778.1 hypothetical protein -
  BRN32_RS08345 (BRN32_08345) - 1619278..1619484 (-) 207 WP_027703281.1 YhfG family protein -
  BRN32_RS08350 (BRN32_08350) pilR 1619557..1621008 (-) 1452 WP_178383148.1 sigma-54 dependent transcriptional regulator Regulator
  BRN32_RS08355 (BRN32_08355) - 1621279..1622892 (-) 1614 WP_011258366.1 HAMP domain-containing sensor histidine kinase -
  BRN32_RS08360 (BRN32_08360) sucC 1623126..1624295 (+) 1170 WP_011258367.1 ADP-forming succinate--CoA ligase subunit beta -
  BRN32_RS08365 (BRN32_08365) sucD 1624320..1625195 (+) 876 WP_011258368.1 succinate--CoA ligase subunit alpha -
  BRN32_RS08375 (BRN32_08375) - 1625440..1625736 (+) 297 WP_011408075.1 hypothetical protein -
  BRN32_RS08380 (BRN32_08380) - 1625794..1626015 (+) 222 WP_264484155.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14442.76 Da        Isoelectric Point: 9.0090

>NTDB_id=306939 BRN32_RS08315 WP_011408068.1 1612645..1613070(+) (pilA2) [Xanthomonas oryzae pv. oryzae strain OS198]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVTETASSLGGVANVTLANSGYSFPGATK
YVSNVTIADGTGLVTVTSTVPNAAGTILLTPKDVGGGQLKWTCSSAILTKYLPAECRSSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=306939 BRN32_RS08315 WP_011408068.1 1612645..1613070(+) (pilA2) [Xanthomonas oryzae pv. oryzae strain OS198]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCTGCCATCGCGCT
GCCGGCTTATCAGGACTACACCACTCGTGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCGCCTGCCAAACTTGCGGTAA
CGGAGACTGCATCGTCGCTCGGCGGTGTTGCTAATGTTACTCTCGCGAATTCGGGCTACTCATTCCCGGGTGCCACGAAG
TATGTCAGCAACGTCACCATCGCCGATGGTACGGGCCTAGTCACTGTAACTTCTACCGTGCCGAACGCAGCCGGTACCAT
CCTGCTGACCCCGAAGGATGTCGGTGGCGGCCAGCTGAAGTGGACCTGCTCTTCCGCCATCCTCACCAAGTACCTGCCGG
CGGAGTGCCGTAGCTCGGGCACCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.471

96.454

0.496
  pilA2 Legionella pneumophila strain ERS1305867

51.471

96.454

0.496
  pilA Ralstonia pseudosolanacearum GMI1000

41.818

100

0.489
  comP Acinetobacter baylyi ADP1

43.537

100

0.454
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.054

100

0.447
  pilE Neisseria gonorrhoeae strain FA1090

37.278

100

0.447
  pilE Neisseria gonorrhoeae MS11

37.5

100

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.446

98.582

0.418
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.222

95.745

0.404
  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.397
  pilA Acinetobacter baumannii strain A118

38.298

100

0.383
  pilA Pseudomonas aeruginosa PAK

36

100

0.383
  pilA Vibrio parahaemolyticus RIMD 2210633

40.157

90.071

0.362

Multiple sequence alignment