Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   DV130_RS09240 Genome accession   NZ_CP031248
Coordinates   1724333..1725274 (+) Length   313 a.a.
NCBI ID   WP_000249571.1    Uniprot ID   A0A0B7M3Q7
Organism   Streptococcus pneumoniae strain M26365     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1719333..1730274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV130_RS09220 - 1719399..1721216 (+) 1818 WP_001220844.1 acyltransferase family protein -
  DV130_RS09225 nagA 1721369..1722520 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  DV130_RS09230 - 1722683..1723741 (+) 1059 WP_000649470.1 zinc-dependent alcohol dehydrogenase family protein -
  DV130_RS09235 - 1723892..1724257 (+) 366 WP_000286415.1 DUF1033 family protein -
  DV130_RS09240 comGA/cglA/cilD 1724333..1725274 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  DV130_RS09245 comGB/cglB 1725222..1726238 (+) 1017 WP_078130847.1 competence type IV pilus assembly protein ComGB Machinery gene
  DV130_RS09250 comGC/cglC 1726240..1726563 (+) 324 WP_000738628.1 comG operon protein ComGC Machinery gene
  DV130_RS09255 comGD/cglD 1726526..1726960 (+) 435 WP_033606693.1 competence type IV pilus minor pilin ComGD Machinery gene
  DV130_RS09260 comGE/cglE 1726923..1727225 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  DV130_RS09265 comGF/cglF 1727188..1727649 (+) 462 WP_050072687.1 competence type IV pilus minor pilin ComGF Machinery gene
  DV130_RS09270 comGG/cglG 1727627..1728073 (+) 447 WP_050072685.1 competence type IV pilus minor pilin ComGG Machinery gene
  DV130_RS09275 - 1728176..1728770 (+) 595 Protein_1759 methyltransferase domain-containing protein -
  DV130_RS09280 comYH 1728831..1729784 (+) 954 WP_050072683.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35591.51 Da        Isoelectric Point: 6.0083

>NTDB_id=304864 DV130_RS09240 WP_000249571.1 1724333..1725274(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain M26365]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=304864 DV130_RS09240 WP_000249571.1 1724333..1725274(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain M26365]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCAGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M3Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361

Multiple sequence alignment