Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   DV130_RS09245 Genome accession   NZ_CP031248
Coordinates   1725222..1726238 (+) Length   338 a.a.
NCBI ID   WP_078130847.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain M26365     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1720222..1731238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV130_RS09225 nagA 1721369..1722520 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  DV130_RS09230 - 1722683..1723741 (+) 1059 WP_000649470.1 zinc-dependent alcohol dehydrogenase family protein -
  DV130_RS09235 - 1723892..1724257 (+) 366 WP_000286415.1 DUF1033 family protein -
  DV130_RS09240 comGA/cglA/cilD 1724333..1725274 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  DV130_RS09245 comGB/cglB 1725222..1726238 (+) 1017 WP_078130847.1 competence type IV pilus assembly protein ComGB Machinery gene
  DV130_RS09250 comGC/cglC 1726240..1726563 (+) 324 WP_000738628.1 comG operon protein ComGC Machinery gene
  DV130_RS09255 comGD/cglD 1726526..1726960 (+) 435 WP_033606693.1 competence type IV pilus minor pilin ComGD Machinery gene
  DV130_RS09260 comGE/cglE 1726923..1727225 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  DV130_RS09265 comGF/cglF 1727188..1727649 (+) 462 WP_050072687.1 competence type IV pilus minor pilin ComGF Machinery gene
  DV130_RS09270 comGG/cglG 1727627..1728073 (+) 447 WP_050072685.1 competence type IV pilus minor pilin ComGG Machinery gene
  DV130_RS09275 - 1728176..1728770 (+) 595 Protein_1759 methyltransferase domain-containing protein -
  DV130_RS09280 comYH 1728831..1729784 (+) 954 WP_050072683.1 class I SAM-dependent methyltransferase Machinery gene
  DV130_RS09285 - 1729835..1731025 (+) 1191 WP_000167769.1 acetate/propionate family kinase -
  DV130_RS12790 - 1731026..1731157 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38541.63 Da        Isoelectric Point: 9.8240

>NTDB_id=304865 DV130_RS09245 WP_078130847.1 1725222..1726238(+) (comGB/cglB) [Streptococcus pneumoniae strain M26365]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMKSLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=304865 DV130_RS09245 WP_078130847.1 1725222..1726238(+) (comGB/cglB) [Streptococcus pneumoniae strain M26365]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGTTTGTCTCAGGGGAAATCATTCTCAGAAATGATGAAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTCCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATTTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.675

100

0.947
  comGB/cglB Streptococcus mitis NCTC 12261

93.787

100

0.938
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.599

98.817

0.5

Multiple sequence alignment