Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DACI_RS05410 Genome accession   NC_010002
Coordinates   1176348..1177484 (-) Length   378 a.a.
NCBI ID   WP_012202997.1    Uniprot ID   A0A210VX48
Organism   Delftia acidovorans SPH-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1171348..1182484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DACI_RS05385 (Daci_1061) rsmI 1172344..1173285 (-) 942 WP_012202992.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  DACI_RS05390 (Daci_1062) - 1173285..1173701 (+) 417 WP_012202993.1 YraN family protein -
  DACI_RS05395 (Daci_1063) - 1173775..1174383 (+) 609 WP_012202994.1 SIS domain-containing protein -
  DACI_RS05400 (Daci_1064) - 1174380..1175111 (+) 732 WP_012202995.1 BON domain-containing protein -
  DACI_RS05405 (Daci_1065) - 1175311..1176222 (-) 912 WP_012202996.1 NAD(P)-dependent oxidoreductase -
  DACI_RS05410 (Daci_1066) pilU 1176348..1177484 (-) 1137 WP_012202997.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DACI_RS05415 (Daci_1067) - 1177606..1178238 (-) 633 WP_012202998.1 cyclic nucleotide-binding domain-containing protein -
  DACI_RS05420 (Daci_1068) pilT 1178270..1179313 (-) 1044 WP_012202999.1 type IV pilus twitching motility protein PilT Machinery gene
  DACI_RS05425 (Daci_1069) - 1179351..1180076 (+) 726 WP_012203000.1 YggS family pyridoxal phosphate-dependent enzyme -
  DACI_RS05430 (Daci_1070) - 1180115..1181398 (-) 1284 WP_012203001.1 branched-chain amino acid ABC transporter substrate-binding protein -
  DACI_RS05435 (Daci_1071) - 1181425..1182147 (-) 723 WP_012203002.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41978.48 Da        Isoelectric Point: 6.9076

>NTDB_id=29490 DACI_RS05410 WP_012202997.1 1176348..1177484(-) (pilU) [Delftia acidovorans SPH-1]
MERDQATKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGRITKVSPQPLSPVHTLTLARSVMSDKQIADFERTKECNFAI
SPPGIGRFRVNAFIQQGRVGMVMRTIPLALPTIDSLGVPQVLKEVAMTKRGLCILVGATGSGKSTTLAAMVDWRNESSYG
HIVTIEDPIEFVHPHKNCVVTQREVGLDTDSWDAALKNSLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEDRRPQLLMDLSLNLRAMVSQRLLPKQDSKGRVAAIEVMLNSPLISDLIFKGEVTEIKEIMKKSRNLG
MQTFDQALFDLFESNLVTYEDALRNADSVNDLRLQIKLGSQRAKSLDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=29490 DACI_RS05410 WP_012202997.1 1176348..1177484(-) (pilU) [Delftia acidovorans SPH-1]
ATGGAAAGAGATCAGGCCACCAAGTTCATCAACGACCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACTTGTTCAT
CACGGCGGAATTCCCGCCGGCGATCAAGATCGACGGGCGCATCACCAAGGTGTCGCCGCAGCCGCTGTCGCCCGTGCACA
CGCTGACGCTGGCGCGCTCGGTCATGAGCGACAAGCAGATCGCCGATTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCGCCGCCGGGCATAGGGCGCTTTCGCGTCAACGCCTTCATCCAGCAGGGCCGCGTAGGCATGGTGATGCGGACGATTCC
GCTGGCCCTGCCCACCATCGACAGCCTGGGCGTGCCCCAGGTGCTCAAGGAGGTGGCCATGACCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCCACGGGCTCGGGCAAATCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAGCTCCTACGGC
CACATCGTGACCATCGAGGACCCCATCGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAAGTGGGCCT
GGACACGGACAGCTGGGACGCGGCGCTCAAGAACTCCCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCATCGCCTTCTCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAGGACCGCCGCCCGCAGCTGTTGATGGACCTGTCGCTGAACCT
GCGCGCCATGGTCTCCCAGCGCCTGCTGCCCAAGCAGGACAGCAAGGGCCGCGTGGCCGCCATCGAGGTGATGCTCAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCACCGAGATCAAGGAGATCATGAAGAAAAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGTCCAACCTGGTCACCTACGAGGATGCACTGCGCAATGCCGA
CTCGGTCAACGACCTGCGGCTGCAGATCAAGCTGGGCAGCCAGCGCGCCAAGTCGCTGGACCTGGCGGCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A210VX48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.324

92.328

0.585
  pilU Acinetobacter baylyi ADP1

56.825

94.974

0.54
  pilU Vibrio cholerae strain A1552

53.161

92.063

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

43.62

89.153

0.389
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Acinetobacter nosocomialis M2

42.042

88.095

0.37
  pilT Acinetobacter baumannii D1279779

42.042

88.095

0.37
  pilT Acinetobacter baumannii strain A118

42.042

88.095

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.887

84.392

0.37
  pilT Vibrio cholerae strain A1552

43.887

84.392

0.37

Multiple sequence alignment