Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DCD74_RS08550 Genome accession   NZ_CP029556
Coordinates   1773292..1774437 (+) Length   381 a.a.
NCBI ID   WP_112926946.1    Uniprot ID   -
Organism   Solilutibacter oculi strain 83-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1735331..1772927 1773292..1774437 flank 365


Gene organization within MGE regions


Location: 1735331..1774437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCD74_RS08385 (DCD74_08385) - 1736282..1737157 (-) 876 WP_112926914.1 D-hexose-6-phosphate mutarotase -
  DCD74_RS08390 (DCD74_08390) - 1737157..1737786 (-) 630 WP_112926915.1 CoA transferase subunit B -
  DCD74_RS08395 (DCD74_08395) - 1737788..1738498 (-) 711 WP_112926916.1 CoA transferase subunit A -
  DCD74_RS08400 (DCD74_08400) rfbD 1738643..1739545 (-) 903 WP_112926917.1 dTDP-4-dehydrorhamnose reductase -
  DCD74_RS08405 (DCD74_08405) rfbC 1739542..1740099 (-) 558 WP_112926918.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  DCD74_RS08410 (DCD74_08410) rfbA 1740096..1740980 (-) 885 WP_112926919.1 glucose-1-phosphate thymidylyltransferase RfbA -
  DCD74_RS08415 (DCD74_08415) rfbB 1740993..1742045 (-) 1053 WP_112926920.1 dTDP-glucose 4,6-dehydratase -
  DCD74_RS08420 (DCD74_08420) - 1742054..1743409 (-) 1356 WP_112926921.1 phosphomannomutase -
  DCD74_RS08425 (DCD74_08425) galE 1743425..1744399 (-) 975 WP_112926922.1 UDP-glucose 4-epimerase GalE -
  DCD74_RS08430 (DCD74_08430) - 1744407..1746074 (-) 1668 WP_162615951.1 FkbM family methyltransferase -
  DCD74_RS08435 (DCD74_08435) - 1746082..1747440 (-) 1359 WP_162615952.1 ABC transporter ATP-binding protein -
  DCD74_RS08440 (DCD74_08440) - 1747458..1748270 (-) 813 WP_162615953.1 ABC transporter permease -
  DCD74_RS08445 (DCD74_08445) - 1748270..1749403 (-) 1134 WP_112926926.1 glycosyltransferase family 4 protein -
  DCD74_RS08450 (DCD74_08450) - 1749400..1751628 (-) 2229 WP_112926927.1 glycosyltransferase -
  DCD74_RS08455 (DCD74_08455) - 1751621..1752874 (-) 1254 WP_112926928.1 class I SAM-dependent methyltransferase -
  DCD74_RS08460 (DCD74_08460) - 1752879..1754012 (-) 1134 WP_112926929.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  DCD74_RS08465 (DCD74_08465) - 1754009..1755349 (-) 1341 WP_112926930.1 hypothetical protein -
  DCD74_RS08470 (DCD74_08470) - 1755346..1755732 (-) 387 WP_217424247.1 hypothetical protein -
  DCD74_RS08475 (DCD74_08475) - 1755830..1757239 (-) 1410 WP_112926932.1 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase -
  DCD74_RS08480 (DCD74_08480) - 1757472..1758218 (+) 747 WP_112926933.1 electron transfer flavoprotein subunit beta/FixA family protein -
  DCD74_RS08485 (DCD74_08485) - 1758218..1759159 (+) 942 WP_112926934.1 electron transfer flavoprotein subunit alpha/FixB family protein -
  DCD74_RS12685 - 1759233..1759850 (+) 618 WP_162615956.1 hypothetical protein -
  DCD74_RS08490 (DCD74_08490) - 1759862..1761190 (-) 1329 WP_217424248.1 hypothetical protein -
  DCD74_RS08495 (DCD74_08495) - 1761196..1762836 (-) 1641 WP_112926936.1 DUF6311 domain-containing protein -
  DCD74_RS08500 (DCD74_08500) - 1762833..1763456 (-) 624 WP_112926937.1 hypothetical protein -
  DCD74_RS08505 (DCD74_08505) - 1763453..1763857 (-) 405 WP_112926938.1 GtrA family protein -
  DCD74_RS08510 (DCD74_08510) - 1763841..1764794 (-) 954 WP_112926939.1 glycosyltransferase -
  DCD74_RS08515 (DCD74_08515) - 1764798..1765745 (-) 948 WP_112926940.1 UDP-glucuronic acid decarboxylase family protein -
  DCD74_RS08520 (DCD74_08520) - 1765793..1766692 (-) 900 WP_112926941.1 class I SAM-dependent methyltransferase -
  DCD74_RS08525 (DCD74_08525) - 1766711..1769173 (-) 2463 WP_237049569.1 glycosyltransferase family 1 protein -
  DCD74_RS08530 (DCD74_08530) gmd 1769187..1770158 (-) 972 WP_112926942.1 GDP-mannose 4,6-dehydratase -
  DCD74_RS08535 (DCD74_08535) - 1770148..1771083 (-) 936 WP_112926943.1 GDP-mannose 4,6-dehydratase -
  DCD74_RS08540 (DCD74_08540) - 1771073..1771765 (-) 693 WP_112926944.1 hypothetical protein -
  DCD74_RS08545 (DCD74_08545) - 1771758..1773020 (-) 1263 WP_217424249.1 glycosyltransferase -
  DCD74_RS08550 (DCD74_08550) pilU 1773292..1774437 (+) 1146 WP_112926946.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42323.52 Da        Isoelectric Point: 6.1945

>NTDB_id=293491 DCD74_RS08550 WP_112926946.1 1773292..1774437(+) (pilU) [Solilutibacter oculi strain 83-4]
MDIGHFLKLMTEKHGSDMFLSTGAPIHIKVEGKLYPLGTTSLPPGLVERIAYSLMDREQIEAFERDMELNMAMAIHDAGR
FRVNVFKQRGEVGMVIRAIRTDIPSIEDLQLPQVLKDVITSPRGLVLVVGSTGSGKSTTLASMIDHRNNNMTGHILTIED
PIEFLHRHKRSLVNQREVGIDTHSFHEALRNAMREAPDVILIGEILDAATMEAAITFSETGHLCLATLHSNNADQALERI
LNFFPEAAHKNVLMNLSLNLNAVISQRLVKGVDGKRLPAVEVLLNTPHIRDLMRRGEVHKIKPAMEESLSEQMQTFDQAL
FDMQKQGKIGMEEALRAADSREGLALKFRLSEGSTGEHDAYADVFTSKAPGISQGFDDTNF

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=293491 DCD74_RS08550 WP_112926946.1 1773292..1774437(+) (pilU) [Solilutibacter oculi strain 83-4]
ATGGACATCGGACACTTCCTTAAACTCATGACTGAGAAGCACGGGTCGGACATGTTCCTGTCCACCGGTGCGCCCATCCA
CATCAAGGTGGAAGGCAAATTGTACCCGCTCGGCACCACCAGCCTGCCGCCCGGGCTGGTCGAGCGCATCGCCTACTCGC
TCATGGACCGCGAGCAGATCGAGGCGTTCGAGCGCGACATGGAGCTGAACATGGCCATGGCGATCCACGACGCCGGCCGT
TTCCGCGTGAACGTGTTCAAGCAGCGTGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCACCGATATCCCGAGCATCGA
GGACCTGCAGCTGCCGCAGGTGCTGAAGGACGTCATCACCTCGCCGCGTGGCCTGGTGCTGGTGGTGGGTTCGACCGGCT
CGGGCAAATCGACCACGCTGGCGTCGATGATCGACCACCGCAATAACAACATGACCGGCCACATCCTCACCATCGAGGAC
CCGATAGAGTTCCTGCACCGCCACAAGCGTTCGCTGGTCAACCAGCGCGAGGTCGGCATCGACACCCACAGCTTCCACGA
GGCGCTGAGGAATGCCATGCGCGAGGCGCCCGACGTCATCCTCATCGGCGAGATCCTCGATGCCGCGACGATGGAAGCCG
CGATCACCTTCTCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACTCCAACAACGCCGACCAGGCGCTGGAGCGCATC
CTCAACTTCTTCCCCGAGGCGGCGCACAAGAACGTGCTGATGAACCTCTCGCTCAACCTCAACGCGGTGATCTCGCAGCG
TCTGGTGAAGGGGGTGGACGGCAAGCGCCTGCCGGCGGTGGAGGTGCTGCTCAATACCCCGCACATCCGCGACCTGATGC
GCCGCGGCGAAGTGCACAAGATCAAGCCGGCGATGGAGGAGTCGCTGTCCGAGCAGATGCAGACCTTCGACCAGGCCCTG
TTCGACATGCAGAAGCAGGGCAAGATCGGGATGGAGGAAGCGCTGCGCGCCGCGGATTCACGCGAAGGCCTTGCCCTCAA
GTTCCGTTTGTCCGAAGGCAGCACCGGCGAGCACGATGCCTACGCGGATGTCTTCACCAGCAAGGCACCGGGCATCTCGC
AAGGCTTCGACGACACCAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(35-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

94.226

0.491
  pilU Vibrio cholerae strain A1552

50

92.913

0.465
  pilU Acinetobacter baylyi ADP1

49.153

92.913

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.64

90.289

0.412
  pilT Pseudomonas aeruginosa PAK

42.604

88.714

0.378
  pilT Acinetobacter baumannii strain A118

41.86

90.289

0.378
  pilT Acinetobacter nosocomialis M2

41.86

90.289

0.378
  pilT Acinetobacter baumannii D1279779

41.86

90.289

0.378
  pilT Pseudomonas stutzeri DSM 10701

41.57

90.289

0.375
  pilT Legionella pneumophila strain Lp02

41.716

88.714

0.37
  pilT Legionella pneumophila strain ERS1305867

41.716

88.714

0.37
  pilT Acinetobacter baylyi ADP1

40.988

90.289

0.37

Multiple sequence alignment