Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DGM85_RS05725 Genome accession   NZ_CP029280
Coordinates   1338536..1338976 (+) Length   146 a.a.
NCBI ID   WP_039568292.1    Uniprot ID   A0AB34QK76
Organism   Xanthomonas phaseoli pv. phaseoli strain Px100     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1334181..1348761 1338536..1338976 within 0


Gene organization within MGE regions


Location: 1334181..1348761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DGM85_RS05705 - 1334181..1335251 (-) 1071 WP_157926032.1 RHS repeat domain-containing protein -
  DGM85_RS05710 (DGM85_05995) coaE 1335433..1336044 (-) 612 WP_041019488.1 dephospho-CoA kinase -
  DGM85_RS05715 (DGM85_06000) - 1336055..1336918 (-) 864 WP_039573976.1 A24 family peptidase -
  DGM85_RS05720 (DGM85_06005) pilC 1336925..1338181 (-) 1257 WP_039573972.1 type II secretion system F family protein Machinery gene
  DGM85_RS05725 (DGM85_06010) pilA 1338536..1338976 (+) 441 WP_039568292.1 pilin Machinery gene
  DGM85_RS05730 (DGM85_06015) - 1339086..1339541 (+) 456 WP_039568290.1 pilin -
  DGM85_RS05735 (DGM85_06020) pilB 1339633..1341342 (+) 1710 WP_180959844.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DGM85_RS05740 (DGM85_06030) - 1341587..1341706 (+) 120 WP_039568289.1 hypothetical protein -
  DGM85_RS05745 (DGM85_06035) - 1341768..1341914 (+) 147 WP_080948799.1 serine/threonine protein kinase -
  DGM85_RS05750 (DGM85_06040) pilR 1342111..1343565 (-) 1455 WP_039568287.1 sigma-54 dependent transcriptional regulator Regulator
  DGM85_RS05755 (DGM85_06045) - 1343836..1345449 (-) 1614 WP_039568284.1 HAMP domain-containing sensor histidine kinase -
  DGM85_RS05760 (DGM85_06050) sucC 1345681..1346850 (+) 1170 WP_039568282.1 ADP-forming succinate--CoA ligase subunit beta -
  DGM85_RS05765 (DGM85_06055) sucD 1346875..1347750 (+) 876 WP_005921370.1 succinate--CoA ligase subunit alpha -
  DGM85_RS05770 (DGM85_06060) - 1348212..1348496 (+) 285 Protein_1142 hypothetical protein -
  DGM85_RS05775 (DGM85_06065) - 1348561..1348677 (+) 117 Protein_1143 DUF559 domain-containing protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15218.48 Da        Isoelectric Point: 8.4936

>NTDB_id=291586 DGM85_RS05725 WP_039568292.1 1338536..1338976(+) (pilA) [Xanthomonas phaseoli pv. phaseoli strain Px100]
MKKQHGFTLIELMIVIAIIAILAAIALPQYQNYVAKSQVTAGLAELSPGKTQYETLLNEGKGSTLTTADAVGLQASSTTN
RCSTITVLAPDATTGAQTNALSCTLAGSPRIVGKKITWARDTNGVWTCSTDIATGDKAKFAPATCQ

Nucleotide


Download         Length: 441 bp        

>NTDB_id=291586 DGM85_RS05725 WP_039568292.1 1338536..1338976(+) (pilA) [Xanthomonas phaseoli pv. phaseoli strain Px100]
ATGAAGAAGCAACACGGTTTCACTCTGATCGAACTCATGATCGTCATCGCGATCATTGCAATCTTGGCCGCCATTGCACT
GCCGCAGTACCAGAACTATGTCGCAAAGTCGCAGGTCACTGCTGGTCTGGCCGAGCTGAGCCCGGGCAAGACGCAGTACG
AAACTCTTTTGAACGAAGGCAAGGGCTCGACGCTGACGACTGCTGATGCAGTTGGTCTGCAGGCCTCGTCGACCACCAAT
CGCTGTTCGACGATTACGGTGTTGGCTCCGGATGCTACAACCGGTGCGCAGACTAACGCTTTGAGCTGCACGCTGGCCGG
TAGCCCGCGTATTGTTGGTAAGAAGATCACTTGGGCTCGTGATACCAACGGTGTTTGGACCTGCTCGACTGATATCGCGA
CTGGTGATAAGGCAAAGTTCGCTCCAGCTACCTGTCAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.966

99.315

0.486
  pilA Pseudomonas aeruginosa PAK

43.137

100

0.452
  pilA Vibrio cholerae strain A1552

41.781

100

0.418
  pilA Vibrio cholerae C6706

41.781

100

0.418
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.781

100

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.667

98.63

0.411
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.259

97.945

0.404
  comP Acinetobacter baylyi ADP1

36.709

100

0.397
  pilE Neisseria gonorrhoeae strain FA1090

34.783

100

0.384
  pilA Ralstonia pseudosolanacearum GMI1000

33.133

100

0.377
  pilE Neisseria gonorrhoeae MS11

34.161

100

0.377

Multiple sequence alignment