Detailed information NTG0291587

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DGM85_RS05735
• Genome accession: NZ_CP029280
• Coordinates: 1339633..1341342 (+)
• Length: 569 a.a.
• NCBI ID: WP_180959844.1
• Uniprot ID: -
• Organism: Xanthomonas phaseoli pv. phaseoli strain Px100
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1334181..1348761	1339633..1341342	within	0

Gene organization within MGE regions

Location: 1334181..1348761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DGM85_RS05705	-	1334181..1335251 (-)	1071	WP_157926032.1	RHS repeat domain-containing protein	-
DGM85_RS05710 (DGM85_05995)	coaE	1335433..1336044 (-)	612	WP_041019488.1	dephospho-CoA kinase	-
DGM85_RS05715 (DGM85_06000)	-	1336055..1336918 (-)	864	WP_039573976.1	A24 family peptidase	-
DGM85_RS05720 (DGM85_06005)	pilC	1336925..1338181 (-)	1257	WP_039573972.1	type II secretion system F family protein	Machinery gene
DGM85_RS05725 (DGM85_06010)	pilA	1338536..1338976 (+)	441	WP_039568292.1	pilin	Machinery gene
DGM85_RS05730 (DGM85_06015)	-	1339086..1339541 (+)	456	WP_039568290.1	pilin	-
DGM85_RS05735 (DGM85_06020)	pilB	1339633..1341342 (+)	1710	WP_180959844.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DGM85_RS05740 (DGM85_06030)	-	1341587..1341706 (+)	120	WP_039568289.1	hypothetical protein	-
DGM85_RS05745 (DGM85_06035)	-	1341768..1341914 (+)	147	WP_080948799.1	serine/threonine protein kinase	-
DGM85_RS05750 (DGM85_06040)	pilR	1342111..1343565 (-)	1455	WP_039568287.1	sigma-54 dependent transcriptional regulator	Regulator
DGM85_RS05755 (DGM85_06045)	-	1343836..1345449 (-)	1614	WP_039568284.1	HAMP domain-containing sensor histidine kinase	-
DGM85_RS05760 (DGM85_06050)	sucC	1345681..1346850 (+)	1170	WP_039568282.1	ADP-forming succinate--CoA ligase subunit beta	-
DGM85_RS05765 (DGM85_06055)	sucD	1346875..1347750 (+)	876	WP_005921370.1	succinate--CoA ligase subunit alpha	-
DGM85_RS05770 (DGM85_06060)	-	1348212..1348496 (+)	285	Protein_1142	hypothetical protein	-
DGM85_RS05775 (DGM85_06065)	-	1348561..1348677 (+)	117	Protein_1143	DUF559 domain-containing protein	-

Sequence

Protein

• Length: 569 a.a.
• Molecular weight: 61623.59 Da
• Isoelectric Point: 5.4526

>NTDB_id=291587 DGM85_RS05735 WP_180959844.1 1339633..1341342(+) (pilB) [Xanthomonas phaseoli pv. phaseoli strain Px100]
MGITGIARRLVQDGAVEEAVARSAMDQASAAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLLDVSAFDASQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSALGDDEEGMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQKLFLEAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPDNALLAEGF
TPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEISAIVLEGGNAMQIAEAAQAIGIRDLRQSALMKAANGVTSL
AEINRVTKD

Nucleotide

• Length: 1710 bp

>NTDB_id=291587 DGM85_RS05735 WP_180959844.1 1339633..1341342(+) (pilB) [Xanthomonas phaseoli pv. phaseoli strain Px100]
GTGGGAATTACGGGCATCGCGCGCCGTCTGGTACAGGACGGCGCTGTCGAAGAGGCAGTTGCGCGATCTGCGATGGATCA
GGCATCTGCGGCAAAAGTTCCTCTGCCGCAATGGTTTGCTGAGAAGAAGTTGGTCACTGCTTCCCAGCTCGCCGCTGCTA
ATGCGGTCGAGTTCGGTATGCCGCTGCTGGATGTGTCGGCGTTCGACGCCAGCCAAAACGCGGTCAAGCTGGTGAGTGAG
GAACTACTCCAGAAGCACCAAGTCCTGCCGCTGTTCAAGCGCGGCAACCGGCTGTTCGTGGGGGTGAGCAATCCGACCCA
GACCCGGGCGCTGGATGACATCAAGTTCCACACGAACTTGGTAGTTGAGCCCATCCTTGTGGATGAAGATCAGATCCGTC
GCACCCTGGAGCAATGGCAGGCCAGCAATGCTGCACTGGGCTCCGCGCTCGGTGACGACGAGGAGGGCATGGGGGATCTC
GACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACATCCATTTCGAGCCGTATGAAGACGATT
ACCGGGTGCGCTTGCGCATCGACGGCTTGCTCAAGAACGTGGCCAAGGCGCCGGTGAAGCTGAATCAGCGCATCGCAGCG
CGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGATGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCCTGTTCGGTGAGAAGGTGGTGCTACGTATCCTGGACGGCA
GCGCGGCCAAGCTGGGTATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAGTTGTTCCTGGAAGCGATCCACAAGCCG
TACGGGATGGTACTGGTGACCGGGCCGACTGGCTCGGGCAAGACGGTCTCGCTGTACACGGCGCTGGGTATCCTCAATGA
CGAGACGCGCAATATCTCCACTGCGGAAGACCCGGTCGAAATCCGCTTGCCTGGTGTCAACCAGGTGCAGCAGAACAACA
AGCGTGGCATGACCTTCGCCGCAGCCTTGCGCTCGTTCCTGCGACAGGATCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCCCAGACCGGTCACATGGTGCTGTCGACGTTGCACACCAACGATGC
GCCACAGACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCCTGGTCATCGCGC
AGCGGCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCTGATAACGCATTGCTGGCCGAAGGATTC
ACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTTGGCTGCGACGAGTGCACCGAAGGCTACAAGGGGCG
GACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCAGTGCGATCGTGCTGGAAGGCGGCAATGCAATGCAGATCG
CCGAGGCCGCGCAGGCGATCGGTATCCGAGATTTGCGTCAATCGGCGCTGATGAAGGCTGCGAATGGGGTCACCAGCCTG
GCAGAGATCAACCGGGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (40-144)

• Type II/IV secretion system protein (T2SSE) (192-459)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.69	99.824	0.566
pilB	Acinetobacter baylyi ADP1	55.81	99.824	0.557
pilB	Legionella pneumophila strain ERS1305867	51.67	100	0.517
pilB	Vibrio cholerae strain A1552	50.181	97.364	0.489
pilF	Neisseria gonorrhoeae MS11	48.944	99.824	0.489
pilB	Vibrio parahaemolyticus RIMD 2210633	47.143	98.418	0.464
pilB	Vibrio campbellii strain DS40M4	45.694	100	0.457
pilF	Thermus thermophilus HB27	39.615	91.388	0.362
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.048	92.267	0.36