Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LLG50_RS10740 Genome accession   NZ_CP025500
Coordinates   2173105..2174043 (-) Length   312 a.a.
NCBI ID   WP_033900707.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain G50     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2168105..2179043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLG50_RS10700 (LLG50_10700) - 2168993..2169835 (-) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  LLG50_RS10705 (LLG50_10705) - 2169832..2170269 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  LLG50_RS10710 (LLG50_10710) comGG 2170350..2170634 (-) 285 WP_017865155.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLG50_RS10715 (LLG50_10715) comGF 2170673..2171119 (-) 447 WP_058203710.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLG50_RS10720 (LLG50_10720) comGE 2171082..2171378 (-) 297 WP_033900709.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLG50_RS10725 (LLG50_10725) comGD 2171350..2171781 (-) 432 WP_103055168.1 competence type IV pilus minor pilin ComGD Machinery gene
  LLG50_RS10730 (LLG50_10730) comGC 2171741..2172124 (-) 384 WP_012898622.1 competence type IV pilus major pilin ComGC Machinery gene
  LLG50_RS10735 (LLG50_10735) comGB 2172138..2173211 (-) 1074 WP_080735950.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLG50_RS10740 (LLG50_10740) comGA 2173105..2174043 (-) 939 WP_033900707.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35691.92 Da        Isoelectric Point: 6.1811

>NTDB_id=262118 LLG50_RS10740 WP_033900707.1 2173105..2174043(-) (comGA) [Lactococcus lactis subsp. lactis strain G50]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=262118 LLG50_RS10740 WP_033900707.1 2173105..2174043(-) (comGA) [Lactococcus lactis subsp. lactis strain G50]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCTGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

77.244

100

0.772
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.679

0.548
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.412

99.679

0.522
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.757

100

0.519

Multiple sequence alignment