Detailed information NTG0262113

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLG50_RS10715
• Genome accession: NZ_CP025500
• Coordinates: 2170673..2171119 (-)
• Length: 148 a.a.
• NCBI ID: WP_058203710.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain G50
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2165673..2176119

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLG50_RS10680 (LLG50_10680)	-	2165791..2166168 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
LLG50_RS10685 (LLG50_10685)	-	2166373..2167239 (+)	867	WP_103055167.1	RluA family pseudouridine synthase	-
LLG50_RS10690 (LLG50_10690)	-	2167277..2168086 (-)	810	WP_014570791.1	metal ABC transporter permease	-
LLG50_RS10695 (LLG50_10695)	-	2168079..2168816 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
LLG50_RS10700 (LLG50_10700)	-	2168993..2169835 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
LLG50_RS10705 (LLG50_10705)	-	2169832..2170269 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
LLG50_RS10710 (LLG50_10710)	comGG	2170350..2170634 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
LLG50_RS10715 (LLG50_10715)	comGF	2170673..2171119 (-)	447	WP_058203710.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLG50_RS10720 (LLG50_10720)	comGE	2171082..2171378 (-)	297	WP_033900709.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLG50_RS10725 (LLG50_10725)	comGD	2171350..2171781 (-)	432	WP_103055168.1	competence type IV pilus minor pilin ComGD	Machinery gene
LLG50_RS10730 (LLG50_10730)	comGC	2171741..2172124 (-)	384	WP_012898622.1	competence type IV pilus major pilin ComGC	Machinery gene
LLG50_RS10735 (LLG50_10735)	comGB	2172138..2173211 (-)	1074	WP_080735950.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLG50_RS10740 (LLG50_10740)	comGA	2173105..2174043 (-)	939	WP_033900707.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17181.02 Da
• Isoelectric Point: 9.0871

>NTDB_id=262113 LLG50_RS10715 WP_058203710.1 2170673..2171119(-) (comGF) [Lactococcus lactis subsp. lactis strain G50]
MERKLCNLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFL
YVTKDKKLRFGFMRDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=262113 LLG50_RS10715 WP_058203710.1 2170673..2171119(-) (comGF) [Lactococcus lactis subsp. lactis strain G50]
ATGGAAAGGAAATTATGCAACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTG
TATGTAACTAAAGACAAAAAACTAAGGTTTGGTTTTATGAGAGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAGAGAACATTTATTTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	41.606	92.568	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385
comGF/cglF	Streptococcus pneumoniae Rx1	39.007	95.27	0.372
comGF/cglF	Streptococcus pneumoniae D39	39.007	95.27	0.372
comGF/cglF	Streptococcus pneumoniae R6	39.007	95.27	0.372
comGF/cglF	Streptococcus pneumoniae TIGR4	39.007	95.27	0.372