Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PCRYO_RS01525 Genome accession   NC_007969
Coordinates   349971..351092 (-) Length   373 a.a.
NCBI ID   WP_011512664.1    Uniprot ID   Q1QE27
Organism   Psychrobacter cryohalolentis K5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 344971..356092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCRYO_RS01495 (Pcryo_0286) - 345521..345856 (+) 336 WP_011512658.1 c-type cytochrome -
  PCRYO_RS01500 (Pcryo_0287) - 346191..346859 (+) 669 WP_011512659.1 c-type cytochrome -
  PCRYO_RS01505 (Pcryo_0288) - 347124..348266 (+) 1143 WP_011512660.1 hypothetical protein -
  PCRYO_RS01510 (Pcryo_0289) - 348254..348658 (+) 405 WP_011512661.1 RnfH family protein -
  PCRYO_RS01515 (Pcryo_0290) - 348684..349100 (-) 417 WP_011512662.1 outer membrane protein assembly factor BamE -
  PCRYO_RS01520 (Pcryo_0291) fur 349425..349859 (+) 435 WP_011512663.1 ferric iron uptake transcriptional regulator -
  PCRYO_RS01525 (Pcryo_0292) pilU 349971..351092 (-) 1122 WP_011512664.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PCRYO_RS01530 (Pcryo_0293) pilT 351251..352303 (-) 1053 WP_011512665.1 type IV pilus twitching motility protein PilT Machinery gene
  PCRYO_RS01535 (Pcryo_0294) - 352623..353381 (+) 759 WP_041752932.1 YggS family pyridoxal phosphate-dependent enzyme -
  PCRYO_RS01540 (Pcryo_0295) - 353600..354874 (-) 1275 WP_011512667.1 hemolysin family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41686.96 Da        Isoelectric Point: 6.9359

>NTDB_id=25762 PCRYO_RS01525 WP_011512664.1 349971..351092(-) (pilU) [Psychrobacter cryohalolentis K5]
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTVNQQKEFDETNECQFAITDQAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNGVANLDEGADSLSLDIT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=25762 PCRYO_RS01525 WP_011512664.1 349971..351092(-) (pilU) [Psychrobacter cryohalolentis K5]
ATGGACATTGATAAACTTTTACAATTGATGATTAACAAAAACGGCTCTGACCTTTTTATCACTGCTGATGTCGCCCCTTC
CATGAAAATCAATGGCAAAATACTGCCGGTTGGTAAAACGCCTTTAACTGCTGAGCAGACTATGAGGCTGGTTAAAGGTG
TCATGACAGTCAATCAGCAAAAAGAGTTTGATGAGACCAACGAGTGCCAGTTTGCGATTACTGATCAGGCGCAACAAGCA
CGTTTTAGGGTCAGTGCCTTTATACAACGTGATATGGCAGGGATGATTTTACGGAAAATCGAAAACAAAATCCCAACGGT
TGAAGAGCTGCGCCTGCCACCAGCATTAAAAGAGCTGGCAATGAAGAAGCGCGGTATTATTTTGCTGGTTGGTGCAACTG
GTACGGGTAAATCAACCACCCTTGCAGCAATGATAGGACATCGCAATCAAAACTCTCACGGTCATATTATTACCATAGAA
GATCCTATCGAGTTTGTCCATAAGCATCGTGGCTGTATTATTACCCAGCGCGAAGTCGGTATCGATACCCACTCTTTTGA
AGCCGGTCTAAAAAACACCTTGCGCCAAGCGCCAGATGTGATTTTGATTGGTGAGATTCGCAATCGTGAAGTCATGAGTT
ACGCGATTCAATATGCTGAAACGGGGCATTTGGTATTTGCAACGTTGCACGCCAATAATGCCAACCAAGCAATCGACCGC
ATTATTCACTTTTTTGAGACCAGTCGCCATAATCAATTGTTTATGGATTTATCGCTTAACTTACAAGCCATTATCGCCCA
GCAGTTGATTCCAACGCCTGACGGCAAGGGACGCCGGGCTGCTATCGAAATCTTGCTAAACTCACAGCTGATCAGCGACT
ATATCCGCAAAGGTGAGGTGCACGAAATAAAAGATGTCATGACACGCTCACGTGATAGCGGTATGCAGACATTTGACCAA
GCACTGTTTGATCTATATGAAAACGGTGAGATTACTTATAAAGAAGCGATTCTTCATGCAGACTCGCCTAATGATCTGCG
CTTAAAAATCAAACTCAGCAGCAAAAATGGCGTGGCAAACTTAGATGAAGGCGCGGATAGTTTATCGTTAGACATTACTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1QE27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.952

100

0.66
  pilU Acinetobacter baylyi ADP1

67.042

95.174

0.638
  pilU Vibrio cholerae strain A1552

52.174

98.66

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.803

98.123

0.41
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.194

89.812

0.37
  pilT Vibrio cholerae strain A1552

41.194

89.812

0.37
  pilT Acinetobacter nosocomialis M2

39.942

91.957

0.367
  pilT Acinetobacter baumannii D1279779

39.942

91.957

0.367
  pilT Acinetobacter baumannii strain A118

39.942

91.957

0.367
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362
  pilT Legionella pneumophila strain Lp02

41.538

87.131

0.362
  pilT Legionella pneumophila strain ERS1305867

41.538

87.131

0.362

Multiple sequence alignment