Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CUN60_RS00670 Genome accession   NZ_CP024847
Coordinates   142820..143224 (-) Length   134 a.a.
NCBI ID   WP_102950173.1    Uniprot ID   A0A2I7N345
Organism   Aquella oligotrophica strain DSM 100970     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 137820..148224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUN60_RS00650 (CUN60_00650) - 138375..139286 (-) 912 WP_102950169.1 LysR family transcriptional regulator -
  CUN60_RS00655 (CUN60_00655) rsmA 139430..140185 (+) 756 WP_102950170.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
  CUN60_RS00660 (CUN60_00660) - 140271..142223 (-) 1953 WP_102950171.1 acetoacetate--CoA ligase -
  CUN60_RS00665 (CUN60_00665) - 142315..142830 (-) 516 WP_102950172.1 shikimate kinase -
  CUN60_RS00670 (CUN60_00670) pilQ 142820..143224 (-) 405 WP_102950173.1 hypothetical protein Machinery gene
  CUN60_RS00675 (CUN60_00675) - 143113..145032 (-) 1920 WP_102950174.1 AMIN domain-containing protein -
  CUN60_RS00680 (CUN60_00680) - 145029..145517 (-) 489 WP_158649222.1 hypothetical protein -
  CUN60_RS00685 (CUN60_00685) pilO 145514..146113 (-) 600 WP_102950176.1 type 4a pilus biogenesis protein PilO -
  CUN60_RS00690 (CUN60_00690) - 146085..146663 (-) 579 WP_102950177.1 PilN domain-containing protein -
  CUN60_RS00695 (CUN60_00695) pilM 146650..147738 (-) 1089 WP_158649223.1 pilus assembly protein PilM -
  CUN60_RS00700 (CUN60_00700) - 147804..148034 (-) 231 WP_102950179.1 hypothetical protein -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 14664.56 Da        Isoelectric Point: 4.5932

>NTDB_id=255841 CUN60_RS00670 WP_102950173.1 142820..143224(-) (pilQ) [Aquella oligotrophica strain DSM 100970]
MPYQQASSAGNTNVAFVNATLSLQVTPQITDDGFILLNINIQKNTPSPTLQVQGTPAINTNSVTTQVRVKDGSTILVGGI
YVDDQQKVQEQIPYLGDIPYLGWLFKAQQTTNSKRELLIFITPRVIANSLENDS

Nucleotide


Download         Length: 405 bp        

>NTDB_id=255841 CUN60_RS00670 WP_102950173.1 142820..143224(-) (pilQ) [Aquella oligotrophica strain DSM 100970]
ATTCCATATCAACAAGCTTCAAGTGCGGGAAATACCAACGTTGCTTTTGTCAATGCAACTTTATCGTTGCAGGTTACTCC
GCAAATTACCGATGATGGTTTTATTTTGCTAAATATCAATATACAAAAAAATACACCTAGTCCAACTTTGCAGGTGCAAG
GAACTCCAGCGATTAATACCAATAGTGTTACTACTCAAGTACGAGTTAAAGATGGTTCAACCATTTTGGTTGGTGGTATT
TATGTTGATGATCAACAGAAGGTTCAAGAACAAATCCCATATTTGGGAGACATACCATACTTGGGATGGTTGTTTAAAGC
TCAGCAAACGACTAACTCTAAACGTGAATTATTGATTTTTATCACTCCACGTGTAATTGCGAATAGTTTAGAAAATGACA
GCTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(5-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I7N345

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

52.308

97.015

0.507
  pilQ Legionella pneumophila strain ERS1305867

52.308

97.015

0.507
  pilQ Neisseria gonorrhoeae strain FA1090

44.275

97.761

0.433
  pilQ Pseudomonas aeruginosa PAK

45.312

95.522

0.433
  comQ Acinetobacter baylyi ADP1

39.85

99.254

0.396
  pilQ Vibrio campbellii strain DS40M4

41.085

96.269

0.396
  pilQ Vibrio cholerae strain A1552

39.844

95.522

0.381
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.844

95.522

0.381
  pilQ Acinetobacter baumannii strain A118

38.346

99.254

0.381
  pilQ Acinetobacter baumannii D1279779

38.346

99.254

0.381
  pilQ Neisseria meningitidis MC58

40.8

93.284

0.381
  pilQ Neisseria meningitidis M1080

40.8

93.284

0.381
  comE Glaesserella parasuis strain SC1401

38.281

95.522

0.366

Multiple sequence alignment