Detailed information NTG0001100

Experimentally validated

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: NMB_RS09455
• Genome accession: NC_003112
• Coordinates: 1901643..1903952 (+)
• Length: 769 a.a.
• NCBI ID: WP_002225652.1
• Uniprot ID: Q70M91
• Organism: Neisseria meningitidis MC58
• Function: pseudopilus extrusion and retraction DNA binding and uptake

Function

PilQ structure exhibits a dynamic response to the binding of its transported substrate and suggest that the secretin could play an active role in type IV pilus assembly as well as secretion. In vitro experiments using recombinant fragments of PilP and PilQ showed that the N-terminal region of PilP interacted with the middle part of the PilQ polypeptide.

Genomic Context

Location: 1896643..1908952

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMB_RS09435 (NMB1808)	pilM	1898696..1899811 (+)	1116	WP_002214517.1	type IV pilus biogenesis protein PilM	-
NMB_RS09440 (NMB1809)	pilN	1899814..1900413 (+)	600	WP_002214520.1	PilN domain-containing protein	Machinery gene
NMB_RS09445 (NMB1810)	-	1900414..1901061 (+)	648	WP_002217908.1	type 4a pilus biogenesis protein PilO	-
NMB_RS09450 (NMB1811)	pilP	1901079..1901624 (+)	546	WP_002214522.1	pilus assembly protein PilP	Machinery gene
NMB_RS09455 (NMB1812)	pilQ	1901643..1903952 (+)	2310	WP_002225652.1	type IV pilus secretin PilQ	Machinery gene
NMB_RS09460 (NMB1813)	-	1904854..1905366 (+)	513	WP_002222978.1	shikimate kinase	-
NMB_RS09465 (NMB1814)	aroB	1905446..1906525 (+)	1080	WP_002221481.1	3-dehydroquinate synthase	-
NMB_RS09470 (NMB1815)	-	1907492..1908334 (+)	843	WP_010980995.1	class II glutamine amidotransferase	-
NMB_RS09475 (NMB1816)	nrdR	1908364..1908828 (+)	465	WP_002217912.1	transcriptional regulator NrdR	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
dsbA1	pilQ	positive effect
dsbA2	pilQ	positive effect

Sequence

Protein

• Length: 769 a.a.
• Molecular weight: 82473.09 Da
• Isoelectric Point: 10.0982

>NTDB_id=1100 NMB_RS09455 WP_002225652.1 1901643..1903952(+) (pilQ) [Neisseria meningitidis MC58]
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQQAAAPAKQTNIDFRKDGKNAGII
ELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYF
TFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
ARNLDMRQQGNIVNIAPRDELLAKDKALLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLISGRGS
VLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGF
GGDDKWGAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIAN
GGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNG
NTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY

Nucleotide

• Length: 2310 bp

>NTDB_id=1100 NMB_RS09455 WP_002225652.1 1901643..1903952(+) (pilQ) [Neisseria meningitidis MC58]
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACAGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCTCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTCTGGTTCTGAATCTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCACGCCCCGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTCTACCAAGTCCGCAGTA
TCCGTATCCGAACCCTTTACCCCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTATCCGTATCCGCACC
GTTCAGCCCGGCAAAACAACAGGCGGCGGCATCAGCAAAACAACAGGCGGCAGCACCAGCAAAACAACAGGCGGCAGCAC
CAGCAAAACAACAGGCGGCAGCACCAGCAAAACAAACCAATATCGATTTCCGCAAAGACGGCAAAAATGCCGGCATTATC
GAATTGGCTGCATTGGGCTTTGCCGGGCAGCCCGACATCAGCCAACAGCACGACCACATCATCGTTACGCTGAAAAACCA
TACCCTGCCGACCACGCTCCAACGCAGTTTGGATGTGGCAGACTTTAAAACACCGGTTCAAAAGGTTACGCTGAAACGCC
TCAATAACGACACCCAGCTGATTATCACAACAGCCGGCAACTGGGAACTCGTCAACAAATCCGCCGCGCCCGGATACTTT
ACCTTCCAAGTCCTGCCGAAAAAACAAAACCTCGAGTCAGGCGGCGTGAACAATGCGCCCAAAACCTTCACAGGCCGGAA
AATCTCCCTTGACTTCCAAGATGTCGAAATCCGCACCATCCTGCAGATTTTGGCAAAAGAATCCGGAATGAACATTGTTG
CCAGCGACTCCGTCAACGGCAAAATGACCCTCTCCCTCAAGGATGTGCCTTGGGATCAGGCTTTGGATTTGGTTATGCAG
GCGCGCAACCTCGATATGCGCCAGCAAGGGAATATCGTCAACATCGCGCCCCGCGACGAGCTGCTTGCCAAAGACAAAGC
CCTCTTACAGGCAGAAAAAGACATTGCCGATTTGGGTGCGCTGTATTCCCAAAACTTCCAGTTGAAATACAAAAATGTGG
AAGAATTCCGCAGCATCCTGCGTTTGGACAATGCCGACACGACCGGAAACCGCAACACGCTTATCAGCGGCAGGGGCAGC
GTGCTGATCGATCCCGCCACCAACACCCTGATTGTTACCGACACCCGCAGCGTCATCGAAAAATTCCGCAAACTGATTGA
CGAATTGGACGTACCCGCGCAACAAGTGATGATTGAGGCGCGTATCGTCGAAGCGGCAGACGGCTTCTCGCGCGATTTGG
GCGTTAAATTCGGCGCGACAGGCAAGAAAAAGCTGAAAAATGATACAAGCGCATTCGGCTGGGGGGTAAACTCCGGCTTC
GGCGGCGACGATAAATGGGGGGCCGAAACCAAAATCAACCTGCCGATTACCGCTGCCGCAAACAGCATTTCGCTGGTGCG
CGCGATTTCCTCCGGTGCCTTGAATTTGGAATTGTCCGCATCCGAATCGCTTTCAAAAACCAAAACGCTTGCCAATCCGC
GCGTGCTGACCCAAAACCGCAAAGAGGCCAAAATCGAATCCGGTTACGAAATTCCTTTCACCGTAACCTCAATCGCGAAC
GGCGGCAGCAGCACGAACACGGAACTCAAAAAAGCCGTCTTGGGGCTGACCGTTACGCCGAACATCACGCCCGACGGCCA
AATCATTATGACCGTCAAAATCAACAAGGACTCGCCTGCGCAATGTGCCTCCGGTAATCAGACGATCCTGTGTATTTCGA
CCAAAAACCTGAATACGCAGGCTATGGTTGAAAACGGCGGCACATTGATTGTCGGCGGTATTTATGAAGAAGACAACGGC
AATACGCTGACCAAAGTCCCCCTGTTGGGCGACATCCCCGTTATCGGCAACCTCTTTAAAACACGCGGGAAAAAAACCGA
CCGCCGCGAACTGCTGATTTTCATTACCCCGAGGATTATGGGTACGGCCGGCAACAGCCTGCGCTATTGA

Domains

Predicted by InterproScan.

• Bacterial type II/III secretion system short domain (Secretin_N) (444-515)

• Bacterial type II and III secretion system protein (Secretin) (601-760)

• Secretin and TonB N terminus short domain (STN) (371-416)

• AMIN domain (AMIN) (43-116)

3D structure

• PDB: 4AQZ
• PDB: 4AV2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Neisseria meningitidis M1080	98.309	100	0.992
pilQ	Neisseria gonorrhoeae strain FA1090	89.987	100	0.957

References

• [1]: Stephan A Frye et al. (2015) The Inner Membrane Protein PilG Interacts with DNA and the Secretin PilQ in Transformation. PloS One 10(8):e0134954. [PMID: 26248334]
• [2]: Sunita Sinha et al. (2008) Reduced DNA binding and uptake in the absence of DsbA1 and DsbA2 of Neisseria meningitidis due to inefficient folding of the outer-membrane secretin PilQ. Microbiology (Reading, England) 154(Pt 1):217-225. [PMID: 18174140]
• [3]: Seetha V Balasingham et al. (2007) Interactions between the lipoprotein PilP and the secretin PilQ in Neisseria meningitidis. Journal of Bacteriology 189(15):5716-27. [PMID: 17526700]
• [4]: Richard F Collins et al. (2005) Interaction with type IV pili induces structural changes in the bacterial outer membrane secretin PilQ. The Journal of Biological Chemistry 280(19):18923-30. [PMID: 15753075]