Detailed information    

experimental Experimentally validated

Overview

Name   dsbA2   Type   Machinery gene
Locus tag   NMB_RS01545 Genome accession   NC_003112
Coordinates   303843..304538 (+) Length   231 a.a.
NCBI ID   WP_002215704.1    Uniprot ID   Q7DDR6
Organism   Neisseria meningitidis MC58     
Function   DNA binding and uptake; interact with PilQ   
DNA binding and uptake

Function

The involvement of DsbA1 and DsbA2 in natural competence was confirmed and attributed to events in the early stages of the transformation process. Strains lacking both DsbA1 and DsbA2 were reduced in competence as a result of decreased DNA binding and uptake. Both DsbA1 and DsbA2 were able to specifically bind PilQ in solid-phase overlay assays. Consistent with this, deletion of both dsbA1 and dsbA2 resulted in reduced levels of PilQ, confirming inefficient folding of PilQ, while pilus expression was apparently unaffected.


Genomic Context

Location: 298843..309538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS12095 - 298916..299370 (-) 455 Protein_289 deoxyribodipyrimidine photo-lyase -
  NMB_RS01525 (NMB0290) - 299360..300271 (-) 912 WP_002221945.1 LysR family transcriptional regulator -
  NMB_RS01530 (NMB0291) - 300379..300777 (+) 399 WP_002216361.1 DoxX family protein -
  NMB_RS01535 (NMB0292) - 300826..301431 (+) 606 WP_002230218.1 nitroreductase family protein -
  NMB_RS01540 (NMB0293) - 301617..303728 (+) 2112 WP_002256351.1 TonB-dependent receptor -
  NMB_RS01545 (NMB0294) dsbA2 303843..304538 (+) 696 WP_002215704.1 thiol:disulfide interchange protein DsbA/DsbL Machinery gene
  NMB_RS01550 (NMB0295) ffh 304869..306239 (-) 1371 WP_002215706.1 signal recognition particle protein -
  NMB_RS01555 (NMB0296) - 306459..307265 (+) 807 WP_002221947.1 cytochrome C assembly family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  dsbA2 pilQ positive effect
  dsbA1 pilQ positive effect

Sequence

Protein


Download         Length: 231 a.a.        Molecular weight: 25419.90 Da        Isoelectric Point: 5.1502

>NTDB_id=1099 NMB_RS01545 WP_002215704.1 303843..304538(+) (dsbA2) [Neisseria meningitidis MC58]
MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAELNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCA
HLEPVLSEHIKTFKDDTYMRREHVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQLVDKVREEQKKPQ

Nucleotide


Download         Length: 696 bp        

>NTDB_id=1099 NMB_RS01545 WP_002215704.1 303843..304538(+) (dsbA2) [Neisseria meningitidis MC58]
ATGAAACTGAAAACCTTAGCTTTGACTTCATTGACCCTGTTGGCATTGGCCGCTTGTAGCAAACAGGCTGAAACCAGTGT
TCCGGCAGACAGCGCCCAAAGCAGCTCATCTGCTCCGGCAGCCCCTGCTGAGTTGAACGAAGGTGTGAACTACACTGTAT
TGTCTACGCCTATTCCGCAACAGCAGGCCGGTAAAATCGAAGTATTGGAATTTTTCGGCTACTTCTGCCCGCATTGCGCC
CATCTTGAGCCGGTCTTGAGCGAGCACATCAAAACGTTTAAAGACGATACCTATATGCGCCGGGAGCATGTCGTGTGGGG
TGATGAAATGAAACCTTTGGCACGTTTGGCGGCCGCAGTGGAAATGGCCGGTGAATCAGATAAAGCCAACAGCCATATTT
TCGATGCGATGGTTAATCAAAAAATCAATCTGGCCGATACCGATACCCTGAAAAAATGGCTGTCCGAGCAAACAGCGTTT
GACGGCAAAAAAGTATTGGCTGCATTTGAGGCTCCTGAAAGCCAAGCGCGTGCGGCTCAAATGGAAGAGTTGACCAATAA
ATTCCAAATCAGCGGCACACCGACTGTGATTGTCGGCGGCAAATACCAAGTTGAATTTAAAGACTGGCAGTCCGGTATGA
CCACGATTGACCAGTTGGTGGATAAAGTACGCGAAGAGCAGAAAAAGCCGCAATAA

Domains

Predicted by InterproScan.

Thioredoxin (Thioredoxin_4)

(65-212)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7DDR6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dsbA1 Neisseria meningitidis MC58

71.245

100

0.719

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2008) Reduced DNA binding and uptake in the absence of DsbA1 and DsbA2 of Neisseria meningitidis due to inefficient folding of the outer-membrane secretin PilQ. Microbiology (Reading, England) 154(Pt 1):217-225. [PMID: 18174140]