Detailed information    

experimental Experimentally validated

Overview

Name   dsbA1   Type   Machinery gene
Locus tag   NMB_RS01465 Genome accession   NC_003112
Coordinates   281477..282175 (+) Length   232 a.a.
NCBI ID   WP_002224844.1    Uniprot ID   Q9K189
Organism   Neisseria meningitidis MC58     
Function   DNA binding and uptake; interact with PilQ   
DNA binding and uptake

Function

The involvement of DsbA1 and DsbA2 in natural competence was confirmed and attributed to events in the early stages of the transformation process. Strains lacking both DsbA1 and DsbA2 were reduced in competence as a result of decreased DNA binding and uptake. Both DsbA1 and DsbA2 were able to specifically bind PilQ in solid-phase overlay assays. Consistent with this, deletion of both dsbA1 and dsbA2 resulted in reduced levels of PilQ, confirming inefficient folding of PilQ, while pilus expression was apparently unaffected.


Genomic Context

Location: 276477..287175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS01445 (NMB0275) trpC 277800..278582 (-) 783 WP_002221930.1 indole-3-glycerol phosphate synthase TrpC -
  NMB_RS01450 (NMB0276) - 278633..279589 (-) 957 WP_002224842.1 YheT family hydrolase -
  NMB_RS01455 (NMB0277) murJ 279721..281259 (-) 1539 WP_002224843.1 murein biosynthesis integral membrane protein MurJ -
  NMB_RS01465 (NMB0278) dsbA1 281477..282175 (+) 699 WP_002224844.1 thiol:disulfide interchange protein DsbA/DsbL Machinery gene
  NMB_RS01470 (NMB0279) amgK 282232..283236 (-) 1005 WP_002224845.1 N-acetylmuramate/N-acetylglucosamine kinase AmgK -
  NMB_RS01475 (NMB0280) - 283306..285714 (+) 2409 WP_002243965.1 LPS-assembly protein LptD -
  NMB_RS01480 (NMB0281) - 285819..286862 (+) 1044 WP_050887586.1 peptidylprolyl isomerase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  dsbA1 pilQ positive effect
  dsbA2 pilQ positive effect

Sequence

Protein


Download         Length: 232 a.a.        Molecular weight: 25249.83 Da        Isoelectric Point: 5.6277

>NTDB_id=1098 NMB_RS01465 WP_002224844.1 281477..282175(+) (dsbA1) [Neisseria meningitidis MC58]
MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGLVEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAH
LEPVLSKHAKSFKDDMYLRTEHVVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTA
FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTIDLLADKVREEQKAAQ

Nucleotide


Download         Length: 699 bp        

>NTDB_id=1098 NMB_RS01465 WP_002224844.1 281477..282175(+) (dsbA1) [Neisseria meningitidis MC58]
ATGAAATCCAGACACCTTGCCCTCGGCGTTGCCGCCCTGTTCGCCCTTGCCGCGTGCGACAGCAAAGTCCAAACCAGCGT
CCCCGCCGACAGCGCGCCTGCCGCTTCGGCAGCCGCCGCCCCGGCAGGGCTGGTCGAAGGGCAAAACTATACCGTCCTTG
CCAACCCGATTCCCCAACAGCAGGCAGGCAAAGTCGAAGTCCTTGAGTTTTTCGGCTATTTCTGTCCGCACTGCGCCCAC
CTCGAACCTGTTTTAAGCAAACACGCCAAGTCTTTTAAAGACGATATGTACCTGCGTACCGAACACGTCGTCTGGCAGAA
AGAAATGCTGACGCTGGCACGCCTCGCCGCCGCCGTCGATATGGCTGCCGCCGACAGCAAAGATGTGGCGAACAGCCATA
TTTTCGATGCGATGGTCAACCAAAAAATCAAGCTGCAAAATCCGGAAGTCCTCAAAAAATGGCTGGGCGAACAAACCGCC
TTTGACGGCAAAAAAGTCCTTGCCGCCTACGAGTCCCCCGAAAGCCAGGCGCGCGCCGACAAAATGCAGGAGCTGACCGA
AACCTTCCAAATCGACGGTACGCCCACGGTTATCGTCGGCGGTAAATATAAAGTTGAATTTGCCGACTGGGAGTCCGGTA
TGAACACCATCGACCTTTTGGCGGACAAAGTACGCGAAGAACAAAAAGCCGCGCAGTAA

Domains

Predicted by InterproScan.

Thioredoxin (Thioredoxin_4)

(63-212)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 3A3T
  PDB 3DVW
  PDB 3HZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dsbA2 Neisseria meningitidis MC58

71.245

100

0.719

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2008) Reduced DNA binding and uptake in the absence of DsbA1 and DsbA2 of Neisseria meningitidis due to inefficient folding of the outer-membrane secretin PilQ. Microbiology (Reading, England) 154(Pt 1):217-225. [PMID: 18174140]