Detailed information    

experimental Experimentally validated

Overview

Name   pilP   Type   Machinery gene
Locus tag   NMB_RS09450 Genome accession   NC_003112
Coordinates   1901079..1901624 (+) Length   181 a.a.
NCBI ID   WP_002214522.1    Uniprot ID   Q7DD77
Organism   Neisseria meningitidis MC58     
Function   pseudopilus extrusion and retraction   
DNA binding and uptake

Function

PilP was an inner membrane protein required for pilus expression and transformation, since pilP mutants were nonpiliated and noncompetent. In vitro experiments using recombinant fragments of PilP and PilQ showed that the N-terminal region of PilP interacted with the middle part of the PilQ polypeptide.


Genomic Context

Location: 1896079..1906624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS09430 (NMB1807) - 1896148..1898544 (-) 2397 WP_002219786.1 penicillin-binding protein 1A -
  NMB_RS09435 (NMB1808) pilM 1898696..1899811 (+) 1116 WP_002214517.1 type IV pilus biogenesis protein PilM -
  NMB_RS09440 (NMB1809) pilN 1899814..1900413 (+) 600 WP_002214520.1 PilN domain-containing protein Machinery gene
  NMB_RS09445 (NMB1810) - 1900414..1901061 (+) 648 WP_002217908.1 type 4a pilus biogenesis protein PilO -
  NMB_RS09450 (NMB1811) pilP 1901079..1901624 (+) 546 WP_002214522.1 pilus assembly protein PilP Machinery gene
  NMB_RS09455 (NMB1812) pilQ 1901643..1903952 (+) 2310 WP_002225652.1 type IV pilus secretin PilQ Machinery gene
  NMB_RS09460 (NMB1813) - 1904854..1905366 (+) 513 WP_002222978.1 shikimate kinase -
  NMB_RS09465 (NMB1814) aroB 1905446..1906525 (+) 1080 WP_002221481.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20067.55 Da        Isoelectric Point: 4.6512

>NTDB_id=1101 NMB_RS09450 WP_002214522.1 1901079..1901624(+) (pilP) [Neisseria meningitidis MC58]
MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAPTLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDT
KRIKETLEKFSLENMRYVGILKSGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
LLLNSSDKNTEQAAAPAAEQN

Nucleotide


Download         Length: 546 bp        

>NTDB_id=1101 NMB_RS09450 WP_002214522.1 1901079..1901624(+) (pilP) [Neisseria meningitidis MC58]
ATGAAACACTATGCCTTACTCATCAGCTTTCTGGCTCTCTCCGCGTGTTCCCAAGGTTCTGAGGACCTAAACGAATGGAT
GGCACAAACGCGACGCGAAGCCAAAGCAGAAATCATACCTTTCCAAGCACCTACCCTGCCGGTTGCGCCGGTATACAGCC
CGCCGCAGCTTACAGGGCCGAACGCATTCGACTTCCGCCGCATGGAAACCGACAAAAAAGGGGAAAATGCCCCCGACACC
AAGCGTATTAAAGAAACGCTGGAAAAATTCAGTTTGGAAAATATGCGTTATGTCGGCATTTTGAAGTCCGGACAGAAAGT
CTCCGGCTTCATCGAGGCTGAAGGTTATGTCTACACTGTCGGTGTCGGCAACTATTTGGGACAAAACTACGGTAGAATCG
AAAGCATTACCGACGACAGCATCGTCCTGAACGAGCTAATAGAAGACAGCACGGGCAACTGGGTTTCCCGTAAAGCAGAA
CTGCTGTTGAATTCTTCCGACAAAAACACCGAACAAGCGGCAGCACCTGCCGCAGAACAAAATTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilP (PilP)

(22-163)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 2IVW
  PDB 4AV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Seetha V Balasingham et al. (2007) Interactions between the lipoprotein PilP and the secretin PilQ in Neisseria meningitidis. Journal of Bacteriology 189(15):5716-27. [PMID: 17526700]