Detailed information NTG0001115

Experimentally validated

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: OK783_RS00485
• Genome accession: NZ_CP115654
• Coordinates: 103186..105357 (-)
• Length: 723 a.a.
• NCBI ID: WP_003698035.1
• Uniprot ID: Q5FAD2
• Organism: Neisseria gonorrhoeae strain FA1090
• Function: assembly of type IV pilus DNA binding and uptake

Function

Mutagenesis was performed on the middle one-third of pilQ, and mutants with colony morphologies consistent with the colony morphology of nonpiliated or underpiliated bacteria were selected. Nineteen mutants, each with a single amino acid substitution, were isolated and displayed diverse phenotypes in terms of PilQ multimer stability, pilus expression, transformation efficiency, and host cell adherence.

Genomic Context

Location: 98186..110357

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OK783_RS00470 (OK783_00470)	-	98666..99508 (-)	843	WP_003687345.1	class II glutamine amidotransferase	-
OK783_RS00475 (OK783_00475)	aroB	100247..101326 (-)	1080	WP_003690567.1	3-dehydroquinate synthase	-
OK783_RS00480 (OK783_00480)	-	101406..101918 (-)	513	WP_003687349.1	shikimate kinase	-
OK783_RS00485 (OK783_00485)	pilQ	103186..105357 (-)	2172	WP_003698035.1	type IV pilus secretin PilQ	Machinery gene
OK783_RS00490 (OK783_00490)	pilP	105376..105921 (-)	546	WP_003692554.1	pilus assembly protein PilP	Machinery gene
OK783_RS00495 (OK783_00495)	-	105939..106586 (-)	648	WP_010356664.1	type 4a pilus biogenesis protein PilO	-
OK783_RS00500 (OK783_00500)	pilN	106587..107186 (-)	600	WP_010356666.1	PilN domain-containing protein	Machinery gene
OK783_RS00505 (OK783_00505)	pilM	107189..108304 (-)	1116	WP_003696683.1	type IV pilus biogenesis protein PilM	-

Sequence

Protein

• Length: 723 a.a.
• Molecular weight: 77950.93 Da
• Isoelectric Point: 10.0021

>NTDB_id=1115 OK783_RS00485 WP_003698035.1 103186..105357(-) (pilQ) [Neisseria gonorrhoeae strain FA1090]
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
SVSAPFSPAKQQAAASAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTALQRSLDVADFKTPV
QKVTLKRLNNDTQLIITTTGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAK
ESGMNIVASDSVSGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNF
QLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAA
DGFSRDLGVKFGATGRKKLKNETSAFGWGVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESLSK
TKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGN
NTILCISTKSLNTQAMVENGGTLIVGGIYEENNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNS
LRY

Nucleotide

• Length: 2172 bp

>NTDB_id=1115 OK783_RS00485 WP_003698035.1 103186..105357(-) (pilQ) [Neisseria gonorrhoeae strain FA1090]
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACGGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCTCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTCTGGTTCTGAATTTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCCCGCCCAGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTA
TCCGTATCCGCACCGTTCAGCCCGGCAAAACAACAGGCAGCGGCATCGGCAAAACAAACCAATATCGATTTCCGCAAAGA
CGGCAAAAATGCCGGCATTATCGAATTGGCGGCATTGGGCTTTGCCGGGCAGCCCGACATCAGCCAACAGCACGACCACA
TCATCGTTACGCTGAAAAACCATACCCTGCCGACCGCGCTCCAACGCAGTTTGGATGTGGCAGACTTCAAAACACCGGTT
CAAAAGGTTACGCTGAAACGCCTCAATAACGACACCCAGCTGATTATCACAACAACCGGCAACTGGGAACTCGTCAACAA
ATCCGCCGCGCCCGGATACTTTACCTTCCAAGTCCTGCCGAAAAAACAAAACCTCGAGTCAGGCGGCGTGAACAATGCGC
CCAAAACCTTCACAGGCCGGAAAATCTCCCTTGACTTCCAAGATGTCGAAATCCGCACCATCCTGCAGATTTTGGCAAAA
GAATCCGGGATGAACATTGTTGCCAGCGACTCCGTCAGCGGCAAAATGACCCTCTCCCTCAAAGACGTACCTTGGGATCA
GGCTTTGGATTTGGTTATGCAGGCGCGCAACCTCGATATGCGCCAGCAAGGGAACATCGTCAACATCGCGCCCCGCGACG
AGCTGCTTGCCAAAGACAAAGCCTTCTTACAAGCGGAAAAAGACATTGCCGATCTGGGCGCGCTGTATTCCCAAAACTTC
CAATTGAAATACAAAAATGTGGAAGAATTCCGCAGCATCCTGCGTTTGGACAATGCCGACACGACCGGAAACCGCAACAC
GCTTGTCAGCGGCAGGGGCAGCGTGCTGATCGATCCCGCCACCAACACCCTGATTGTTACCGATACCCGCAGCGTCATCG
AAAAATTCCGCAAACTGATTGACGAATTGGACGTACCCGCGCAACAAGTGATGATTGAGGCGCGTATCGTCGAAGCGGCA
GACGGCTTCTCGCGCGATTTGGGCGTTAAGTTCGGCGCGACAGGCAGGAAAAAACTGAAAAATGAGACGAGCGCATTCGG
CTGGGGCGTGAACTCCGGCTTCGGGGGCGGCGATAAATGGGAGGCCCAAACCAAAATCAACCTGCCGGTTGCCGCTGCCG
CAAACAGCATTTCGCTGGTGCGCGCGATTTCCTCCGGCGCGTTGAATTTGGAATTGTCCGCATCCGAGTCGCTTTCAAAA
ACCAAAACGCTTGCCAATCCGCGCGTGCTGACCCAAAACCGCAAAGAGGCCAAAATCGAATCCGGTTACGAAATTCCTTT
TACCGTAACTACAGCCTCGGGCGGCGGCAACTCTACCAACACGGAACTCAAAAAAGCCGTCTTGGGGCTGACCGTTACGC
CGAACATCACGCCCGACGGACAAATCATCATGACCGTCAAAATCAACAAAGACTCGCCTGCACAATGTGCTTCAGGCAAC
AACACAATCCTATGTATTTCGACCAAAAGCCTGAATACGCAGGCTATGGTTGAAAACGGCGGCACTTTGATTGTCGGCGG
TATTTATGAAGAAAACAACGGCAATACGCTGACCAAAGTCCCCCTGTTGGGCGACATCCCCGTTATCGGCAACCTCTTTA
AAACACGCGGGAAAAAAACCGACCGCCGCGAACTGCTGATTTTCATTACCCCGAGGATTATAGATACGGCCGGCAACAGC
CTGCGCTATTGA

Domains

Predicted by InterproScan.

• Secretin and TonB N terminus short domain (STN) (325-370)

• AMIN domain (AMIN) (43-116)

• Bacterial type II/III secretion system short domain (Secretin_N) (398-469)

• Bacterial type II and III secretion system protein (Secretin) (555-715)

3D structure

• AlphaFold DB: Q5FAD2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Neisseria meningitidis MC58	89.987	100	0.957
pilQ	Neisseria meningitidis M1080	90.802	100	0.956

References

• [1]: R Allen Helm et al. (2007) pilQ Missense mutations have diverse effects on PilQ multimer formation, piliation, and pilus function in Neisseria gonorrhoeae. Journal of Bacteriology 189(8):3198-207. [PMID: 17277065]