Detailed information NTG0001092

Experimentally validated

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: -
• Genome accession: AJ564200
• Coordinates: 1..2286 (+)
• Length: 761 a.a.
• NCBI ID: CAD91899.1
• Uniprot ID: -
• Organism: Neisseria meningitidis M1080
• Function: ssDNA binding DNA processing

Function

DNA associates only weakly with purified pili, but binds significantly to the PilQ complex isolated directly from meningococcal membranes. By assessing the DNA-binding activity of the native complex PilQ, as well as recombinant truncated PilQ monomers, it was shown that the N-terminal region of PilQ is involved in the interaction with DNA. It was evident that the binding of ssDNA to PilQ had a higher affinity than the binding of dsDNA. It is suggested that transforming DNA is introduced into the cell through the outer-membrane channel formed by the PilQ complex, and that DNA uptake occurs by non-specific introduction of DNA coupled to pilus retraction, followed by presentation to DNA-binding component(s), including PilQ.

Genomic Context

Location: 1..7286

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Locus_0	pilQ	1..2286 (+)	2286	CAD91899.1	secretin precursor	Machinery gene

Sequence

Protein

• Length: 761 a.a.
• Molecular weight: 81668.14 Da
• Isoelectric Point: 10.0171

>NTDB_id=1092 CAD91899.1 1..2286(+) (pilQ) [Neisseria meningitidis M1080]
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQTNIDFRKDGKNAGIIELAALGFA
GQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKK
QNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQ
QGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRSILALDNADTTGNRNTLVSGRGSVLIDPATN
TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGA
ETKINLPITAAANSISLVRAISSGALNLELSASESLSKPKTLANPRVLTQNRKEAKIESGYEIPFTVTSIANGGSSTNTE
LKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPL
LGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY*

Nucleotide

• Length: 2286 bp

>NTDB_id=1092 CAD91899.1 1..2286(+) (pilQ) [Neisseria meningitidis M1080]
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACAGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCTCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTCTGGTTCTGAATCTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCACGCCCCGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTCTACCAAGTCCGCAGTA
TCCGTATCCGAACCCTTTACCCCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTATCCGTATCCGCACC
GTTCAGCCCGGCAAAACAACAGGCGGCGGCATCAGCAAAACAACAGACGGCAGCACCAGCAAAACAACAGGCGGCAGCAC
CAGCAAAACAAACCAATATCGATTTCCGCAAAGACGGCAAAAATGCCGGCATTATCGAATTGGCTGCATTGGGCTTTGCC
GGGCAGCCCGACATCAGCCAACAGCACGACCACATCATCGTTACGCTGAAAAACCATACCCTGCCGACCACGCTCCAACG
CAGTTTGGATGTGGCAGACTTTAAAACACCGGTTCAAAAGGTTACGCTGAAACGCCTCAATAACGACACCCAGCTGATTA
TCACAACAGCCGGCAACTGGGAACTCGTCAACAAATCCGCCGCGCCCGGATACTTTACCTTCCAAGTCCTGCCGAAAAAA
CAAAACCTCGAGTCAGGCGGCGTGAACAATGCGCCCAAAACCTTCACAGGCCGGAAAATCTCCCTTGACTTCCAAGATGT
CGAAATCCGCACCATCCTGCAGATTTTGGCAAAAGAATCCGGGATGAACATTGTTGCCAGCGACTCCGTCAACGGCAAAA
TGACCCTCTCCCTCAAAGACGTACCTTGGGATCAGGCTTTGGATTTGGTTATGCAGGCGCGCAACCTCGATATGCGCCAG
CAAGGGAACATCGTCAACATCGCGCCCCGCGACGAGCTGCTTGCCAAAGACAAAGCCTTCTTACAGGCGGAAAAAGACAT
TGCCGATCTGGGCGCGCTGTATTCCCAAAACTTCCAATTGAAATACAAAAATGTGGAAGAATTCCGCAGCATCCTGGCTT
TGGACAATGCCGACACGACCGGAAACCGCAACACGCTTGTCAGCGGCAGGGGCAGCGTGCTGATCGATCCCGCCACCAAT
ACCCTGATTGTTACCGATACCCGCAGCGTCATCGAAAAATTCCGCAAACTGATTGACGAATTGGACGTACCCGCGCAACA
AGTGATGATTGAGGCGCGTATCGTCGAAGCGGCAGACGGCTTCTCGCGCGATTTGGGCGTTAAATTCGGCGCGACAGGCA
AGAAAAAGCTGAAAAATGATACAAGCGCATTCGGCTGGGGGGTAAACTCCGGCTTCGGCGGCGACGATAAATGGGGGGCC
GAAACCAAAATCAACCTGCCGATTACCGCTGCCGCAAACAGCATTTCGCTGGTGCGCGCGATTTCCTCCGGTGCCTTGAA
TTTGGAATTGTCCGCATCCGAATCGCTTTCAAAACCTAAAACGCTTGCCAATCCGCGCGTGCTGACCCAAAACCGCAAAG
AGGCCAAAATCGAATCCGGTTACGAAATTCCTTTCACCGTAACCTCAATCGCGAACGGCGGCAGCAGCACGAACACGGAA
CTCAAAAAAGCCGTCTTGGGGCTGACCGTTACGCCGAACATCACGCCCGACGGCCAAATCATTATGACCGTCAAAATCAA
CAAGGACTCGCCTGCGCAATGTGCCTCCGGTAATCAGACGATCCTGTGTATTTCGACCAAAAACCTGAATACGCAGGCTA
TGGTTGAAAACGGCGGCACATTGATTGTCGGCGGTATTTATGAAGAAGACAACGGCAATACGCTGACCAAAGTCCCCCTG
TTGGGCGACATCCCCGTTATCGGCAACCTCTTTAAAACACGCGGGAAAAAAACCGACCGCCGCGAACTGCTGATTTTCAT
TACCCCGAGGATTATGGGTACGGCCGGCAACAGCCTGCGCTATTGA

Domains

Predicted by InterproScan.

• Bacterial type II/III secretion system short domain (Secretin_N) (436-507)

• AMIN domain (AMIN) (43-116)

• Secretin and TonB N terminus short domain (STN) (363-408)

• Bacterial type II and III secretion system protein (Secretin) (594-752)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Neisseria meningitidis MC58	98.309	100	0.992
pilQ	Neisseria gonorrhoeae strain FA1090	90.802	100	0.956

References

• [1]: Stephan A Frye et al. (2015) The Inner Membrane Protein PilG Interacts with DNA and the Secretin PilQ in Transformation. PloS One 10(8):e0134954. [PMID: 26248334]
• [2]: Emma Lång et al. (2009) Identification of neisserial DNA binding components. Microbiology (Reading, England) 155(Pt 3):852-862. [PMID: 19246756]
• [3]: Reza Assalkhou et al. (2007) The outer membrane secretin PilQ from Neisseria meningitidis binds DNA. Microbiology (Reading, England) 153(Pt 5):1593-1603. [PMID: 17464074]
• [4]: Richard F Collins et al. (2004) Structure of the Neisseria meningitidis outer membrane PilQ secretin complex at 12 A resolution. The Journal of Biological Chemistry 279(38):39750-6. [PMID: 15254043]