Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CK911_RS09720 Genome accession   NZ_CP023817
Coordinates   2230300..2231439 (+) Length   379 a.a.
NCBI ID   WP_042018838.1    Uniprot ID   A0A291TIL7
Organism   Aeromonas sp. CU5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2225300..2236439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK911_RS09690 (CK911_09690) - 2225600..2226664 (+) 1065 WP_098969776.1 3-deoxy-7-phosphoheptulonate synthase -
  CK911_RS09695 (CK911_09695) aac(6') 2226665..2227129 (+) 465 WP_253860407.1 aminoglycoside 6'-N-acetyltransferase -
  CK911_RS09700 (CK911_09700) - 2227152..2227946 (-) 795 WP_172954097.1 ATP-binding cassette domain-containing protein -
  CK911_RS09705 (CK911_09705) btuC 2227933..2228940 (-) 1008 WP_098969777.1 vitamin B12 ABC transporter permease BtuC -
  CK911_RS09710 (CK911_09710) - 2229040..2229771 (-) 732 WP_098969778.1 ABC transporter substrate-binding protein -
  CK911_RS09715 (CK911_09715) - 2229885..2230241 (+) 357 WP_005353120.1 DMT family protein -
  CK911_RS09720 (CK911_09720) pilU 2230300..2231439 (+) 1140 WP_042018838.1 type IVa pilus ATPase TapW Machinery gene
  CK911_RS09725 (CK911_09725) - 2231532..2231981 (-) 450 WP_098969779.1 isoprenylcysteine carboxylmethyltransferase family protein -
  CK911_RS09730 (CK911_09730) alr 2232155..2233381 (+) 1227 WP_098969780.1 alanine racemase -
  CK911_RS21650 (CK911_09735) - 2233514..2233783 (+) 270 WP_367576401.1 transposase DNA-binding-containing protein -
  CK911_RS09740 (CK911_09740) - 2233944..2234159 (+) 216 WP_068977520.1 YdcH family protein -
  CK911_RS09745 (CK911_09745) - 2234322..2235509 (+) 1188 WP_098969782.1 extracellular solute-binding protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42313.37 Da        Isoelectric Point: 6.7330

>NTDB_id=250298 CK911_RS09720 WP_042018838.1 2230300..2231439(+) (pilU) [Aeromonas sp. CU5]
MELRDMLQILAQQDGSDLYLSTGAPPCAKFHGGMRPLNDTPLAPGDVARIAAEIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGSLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKNDHF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=250298 CK911_RS09720 WP_042018838.1 2230300..2231439(+) (pilU) [Aeromonas sp. CU5]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCCAGCAAGATGGCTCGGACCTTTATCTCTCAACCGGAGCCCCTCCCTG
CGCCAAATTCCACGGCGGCATGCGGCCTCTGAACGACACGCCACTGGCGCCCGGCGACGTGGCCAGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAACAGCAGTTCGAGAAGGAGCTGGAGATGAACCTTGCCATCTCCCTCCCCCAGATCGGCCGT
TTCCGGATCAACATCTTCAAGCAACGTAACGAAGTCTCCCTGGTGGCCCGTAATATCAAGACCGAGATCCCCCGCTTTGA
AGACCTGAAACTGCCGCCCGTGCTGCTCGACACCATCATGGAGAAGCGGGGTCTGGTACTGTTTGTCGGCGGTACCGGCT
CGGGCAAGTCCACCTCGCTGGCCGCCCTTATCGATCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTTCATCGCCACCGCAAGAGCATAATCAATCAGCGGGAAGTTGGCGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATCCTTATCGGCGAGATCCGCGATCGCGAGACCATGGAACATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCGCAACTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAAACCACCGATGGCGGCCGCCGGGCAGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGCAGCCTCAAAGAGGTGATGGAAAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTTGAAGGGGTGATTGAGGAGGAAGAAGCAGTTAAGAACGCTGATTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAAGAGAAGGGGCAGATTGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAAGACG
AGAAAAACGATCACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A291TIL7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.684

100

0.538
  pilU Acinetobacter baylyi ADP1

52.394

93.668

0.491
  pilU Vibrio cholerae strain A1552

49.714

92.348

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Legionella pneumophila strain ERS1305867

41.617

88.127

0.367
  pilT Legionella pneumophila strain Lp02

41.617

88.127

0.367
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361

Multiple sequence alignment