Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SUDEN_RS06555 Genome accession   NC_007575
Coordinates   1318846..1319946 (+) Length   366 a.a.
NCBI ID   WP_238374792.1    Uniprot ID   -
Organism   Sulfurimonas denitrificans DSM 1251     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1313846..1324946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SUDEN_RS06525 (Suden_1245) - 1314464..1315177 (-) 714 WP_041672266.1 sulfite exporter TauE/SafE family protein -
  SUDEN_RS06530 (Suden_1246) - 1315180..1315689 (-) 510 WP_011372876.1 hypothetical protein -
  SUDEN_RS06535 (Suden_1247) - 1315959..1316795 (-) 837 WP_011372877.1 tyrosine-type recombinase/integrase -
  SUDEN_RS06540 (Suden_1248) - 1316798..1317793 (-) 996 WP_041672267.1 hypothetical protein -
  SUDEN_RS06545 (Suden_1249) - 1317798..1318385 (-) 588 WP_011372879.1 hypothetical protein -
  SUDEN_RS06550 (Suden_1250) gatC 1318513..1318803 (+) 291 WP_011372880.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  SUDEN_RS06555 (Suden_1251) pilT 1318846..1319946 (+) 1101 WP_238374792.1 type IV pilus twitching motility protein PilT Machinery gene
  SUDEN_RS06560 (Suden_1252) - 1319975..1320964 (-) 990 WP_041672519.1 SAM-dependent methyltransferase -
  SUDEN_RS06565 (Suden_1253) - 1320954..1322177 (-) 1224 WP_011372883.1 TolC family protein -
  SUDEN_RS06570 (Suden_1254) - 1322134..1323318 (-) 1185 WP_011372884.1 aspartate aminotransferase family protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41104.16 Da        Isoelectric Point: 6.8933

>NTDB_id=25018 SUDEN_RS06555 WP_238374792.1 1318846..1319946(+) (pilT) [Sulfurimonas denitrificans DSM 1251]
MYRSVIMTQETNQQIDIKELLKSVLHFESSDLHLVAGSKPQIRIDKALKPLNLHVLSAKEIEDMAYALIEDKQKKIFEDK
NELDFSFELENIGRFRANYYRTIRGIACAFRTIPVDVPPLEKFNNNPIFKELIKRERGLILVTGPTGSGKSTTLASMLHE
INLTENKHIITIEDPVEFVHTNINSIFSQREIGSNTSSFATALKFALRQDPDVILVGEMRDKETISAALTAAETGHIVFG
TLHTNSAPSTINRIIDVFDAKEQAQVRAQLASSLISVISQSLIPRVSGGIIATQEILIANTAIQNLIREDKVHQIYSQMQ
LNQNETHMTTQTDQLTTLLRKGIISKESAIKASNRPEELIRIIHNL

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=25018 SUDEN_RS06555 WP_238374792.1 1318846..1319946(+) (pilT) [Sulfurimonas denitrificans DSM 1251]
ATGTATAGGTCTGTAATAATGACGCAAGAGACAAATCAGCAAATTGATATAAAAGAATTGCTTAAGAGTGTTTTACATTT
TGAATCTTCAGATTTGCACCTTGTTGCTGGCAGTAAGCCTCAAATTAGGATAGATAAGGCTCTAAAACCACTTAATCTAC
ACGTTTTGAGCGCAAAAGAGATAGAAGATATGGCTTATGCGCTTATAGAAGATAAGCAAAAAAAGATTTTTGAAGATAAA
AATGAGCTTGATTTCTCATTTGAACTTGAGAATATTGGTCGTTTTCGTGCCAACTACTATCGTACTATTAGAGGGATTGC
ATGTGCATTTCGCACTATTCCAGTAGATGTACCGCCTCTTGAAAAATTTAACAATAACCCAATATTTAAAGAACTTATAA
AGAGAGAAAGAGGACTTATTTTAGTCACAGGTCCTACTGGCAGTGGTAAATCAACTACACTTGCTTCAATGCTTCATGAA
ATTAATCTCACTGAAAATAAACATATTATTACAATAGAAGATCCAGTTGAGTTTGTTCATACAAATATTAACTCCATTTT
TTCGCAAAGAGAGATTGGCTCAAACACTAGCTCATTTGCAACAGCTCTTAAATTTGCTCTACGACAAGATCCAGATGTAA
TACTAGTTGGTGAGATGAGAGATAAAGAGACAATTTCTGCAGCCCTTACTGCAGCTGAGACAGGTCACATCGTTTTTGGG
ACACTCCATACAAACTCTGCACCTTCAACAATAAACCGTATTATTGATGTTTTTGATGCCAAAGAACAAGCACAAGTCAG
AGCACAACTTGCATCTTCGCTTATATCTGTAATTTCTCAATCTCTTATACCAAGAGTTAGCGGAGGAATTATAGCTACCC
AAGAAATTTTAATAGCAAATACAGCCATTCAAAACTTAATCAGAGAGGATAAGGTTCATCAAATCTACTCTCAAATGCAG
CTAAATCAAAATGAGACACATATGACTACTCAGACAGATCAGCTAACAACTCTATTGCGTAAAGGAATTATCTCAAAAGA
GAGTGCAATAAAAGCTTCAAACAGACCAGAAGAGCTTATTAGAATCATTCATAACCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(22-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.312

93.716

0.481
  pilT Acinetobacter baylyi ADP1

50.867

94.536

0.481
  pilT Acinetobacter baumannii strain A118

50.289

94.536

0.475
  pilT Neisseria gonorrhoeae MS11

50.289

94.536

0.475
  pilT Acinetobacter nosocomialis M2

50.289

94.536

0.475
  pilT Acinetobacter baumannii D1279779

50.289

94.536

0.475
  pilT Legionella pneumophila strain Lp02

51.327

92.623

0.475
  pilT Legionella pneumophila strain ERS1305867

51.327

92.623

0.475
  pilT Neisseria meningitidis 8013

50

94.536

0.473
  pilT Pseudomonas stutzeri DSM 10701

50.146

93.716

0.47
  pilT Vibrio cholerae strain A1552

50.15

90.984

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.15

90.984

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.591

96.175

0.448

Multiple sequence alignment