Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   CO686_RS10185 Genome accession   NZ_CP023507
Coordinates   2046693..2047634 (+) Length   313 a.a.
NCBI ID   WP_096753791.1    Uniprot ID   -
Organism   Streptococcus oralis strain FDAARGOS_367     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2041693..2052634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CO686_RS10165 (CO686_10165) tgt 2041725..2042867 (+) 1143 WP_001285238.1 tRNA guanosine(34) transglycosylase Tgt -
  CO686_RS10170 (CO686_10170) - 2043001..2044818 (+) 1818 WP_001220892.1 acyltransferase family protein -
  CO686_RS10175 (CO686_10175) nagA 2044959..2046110 (+) 1152 WP_001134442.1 N-acetylglucosamine-6-phosphate deacetylase -
  CO686_RS10180 (CO686_10180) - 2046263..2046628 (+) 366 WP_000286399.1 DUF1033 family protein -
  CO686_RS10185 (CO686_10185) comGA/cglA/cilD 2046693..2047634 (+) 942 WP_096753791.1 competence type IV pilus ATPase ComGA Machinery gene
  CO686_RS10190 (CO686_10190) comGB/cglB 2047582..2048598 (+) 1017 WP_096753792.1 competence type IV pilus assembly protein ComGB Machinery gene
  CO686_RS10195 (CO686_10195) comGC/cglC 2048600..2048923 (+) 324 WP_000735800.1 competence type IV pilus major pilin ComGC Machinery gene
  CO686_RS10200 (CO686_10200) comGD/cglD 2048886..2049320 (+) 435 WP_172844179.1 competence type IV pilus minor pilin ComGD Machinery gene
  CO686_RS10205 (CO686_10205) comGE/cglE 2049283..2049585 (+) 303 WP_000413383.1 competence type IV pilus minor pilin ComGE Machinery gene
  CO686_RS10210 (CO686_10210) comGF/cglF 2049548..2050009 (+) 462 WP_000250700.1 competence type IV pilus minor pilin ComGF Machinery gene
  CO686_RS10215 (CO686_10215) comGG/cglG 2049987..2050400 (+) 414 WP_000265645.1 competence type IV pilus minor pilin ComGG Machinery gene
  CO686_RS10220 (CO686_10220) - 2050433..2051023 (+) 591 WP_000679770.1 class I SAM-dependent methyltransferase -
  CO686_RS10225 (CO686_10225) comYH 2051082..2052035 (+) 954 WP_000345140.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35505.37 Da        Isoelectric Point: 5.4218

>NTDB_id=247897 CO686_RS10185 WP_096753791.1 2046693..2047634(+) (comGA/cglA/cilD) [Streptococcus oralis strain FDAARGOS_367]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKVSSLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=247897 CO686_RS10185 WP_096753791.1 2046693..2047634(+) (comGA/cglA/cilD) [Streptococcus oralis strain FDAARGOS_367]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGAAAAGAAGGCCCAGGATATCTATTTTATCCCCAAGGA
AAAGTCCTACGAGCTTCACATGCGGGTTGGAGACGAACGGTGTCTAGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTAGCGGGTATGAACGTAGGAGAGAAGAGACGTAGTCAGCTGGGCTCTTGCGACTATCAG
CATGGGGAGAAGGTGTCTTCTCTGCGTTTGTCTACCGTGGGAGATTATCGGGGGCATGAGAGTTTGGTTATTCGTTTGTT
GCACGATGAGGAACAGGACCTGCATTTCTGGTTTCAGGATATGAACGAACTGGGTGAGCAGTACAGGCAACGGGGGCTCT
ATCTATTTGCAGGTCCAGTAGGAAGTGGCAAGACGACCTTGATGCACGAATTAGCCAAGTCTCTGTTTAAAGGACAGCAG
GTCATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTCCAGTTGCAGTTGAATGAGGCGATTGGATTGAC
CTATGAAAATCTGATCAAACTGTCTCTTCGGCATCGTCCGGACCTCTTGATTATTGGTGAAATTCGGGATAGCGAGACAG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGGGCGACAGTTTTTTCAACCATTCATGCCAAGAGTATCCGAGGTGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAACTAGCAGTTGTCCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAGCAGATTGATCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

90.096

100

0.901
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

89.137

100

0.891
  comGA/cglA/cilD Streptococcus pneumoniae D39

89.137

100

0.891
  comGA/cglA/cilD Streptococcus pneumoniae R6

89.137

100

0.891
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

89.137

100

0.891
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.881

99.361

0.665
  comYA Streptococcus mutans UA140

66.881

99.361

0.665
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.968

99.042

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

43.726

84.026

0.367

Multiple sequence alignment