Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   B8B76_RS26390 Genome accession   NZ_CP023316
Coordinates   5540167..5540616 (-) Length   149 a.a.
NCBI ID   WP_021264133.1    Uniprot ID   A0A509JKL4
Organism   Pseudomonas aeruginosa strain PPF-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5535167..5545616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B8B76_RS26370 (B8B76_26320) ampD 5535859..5536425 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  B8B76_RS26375 (B8B76_26325) - 5536576..5538831 (-) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  B8B76_RS26380 (B8B76_26330) nadC 5539117..5539965 (+) 849 WP_021264134.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B8B76_RS26390 (B8B76_26340) pilA 5540167..5540616 (-) 450 WP_021264133.1 pilin Machinery gene
  B8B76_RS26395 (B8B76_26345) pilB 5540848..5542548 (+) 1701 WP_021264132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B8B76_RS26400 (B8B76_26350) pilC 5542552..5543772 (+) 1221 WP_023101565.1 type 4a pilus biogenesis protein PilC Machinery gene
  B8B76_RS26405 (B8B76_26355) pilD 5543776..5544648 (+) 873 WP_021264129.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  B8B76_RS26410 (B8B76_26360) coaE 5544645..5545256 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  B8B76_RS26415 (B8B76_26365) yacG 5545253..5545453 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15537.82 Da        Isoelectric Point: 7.7818

>NTDB_id=245931 B8B76_RS26390 WP_021264133.1 5540167..5540616(-) (pilA) [Pseudomonas aeruginosa strain PPF-1]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGTNIKIGTDPSTATETYV
GVAANANKLGLIDVKIADTGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=245931 B8B76_RS26390 WP_021264133.1 5540167..5540616(-) (pilA) [Pseudomonas aeruginosa strain PPF-1]
ATGAAAGCTCAAAAAGGCTTTACTTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAATCCGCTGAAGACCACTGTTG
AAGAGTCTCTGTCGCGTGGAATTGCTGGTACCAATATTAAAATTGGTACTGATCCATCTACTGCCACCGAAACATATGTT
GGGGTTGCTGCGAATGCCAACAAGCTGGGCTTGATTGATGTAAAGATTGCTGATACTGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACTTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAATCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A509JKL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

68.667

100

0.691
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.358

100

0.47
  pilA Acinetobacter baumannii strain A118

47.297

99.329

0.47
  comP Acinetobacter baylyi ADP1

43.59

100

0.456
  pilA Vibrio cholerae C6706

42.949

100

0.45
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.949

100

0.45
  pilA Vibrio cholerae strain A1552

42.949

100

0.45
  pilA Vibrio parahaemolyticus RIMD 2210633

45.578

98.658

0.45
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.455

95.973

0.436
  pilA Acinetobacter nosocomialis M2

41.429

93.96

0.389
  pilA2 Legionella pneumophila str. Paris

39.189

99.329

0.389
  pilA2 Legionella pneumophila strain ERS1305867

38.514

99.329

0.383

Multiple sequence alignment